Cohesin gene defects may impair sister chromatid alignment and genome stability in Arabidopsis thaliana.

Abstract:

:In contrast to yeast, plant interphase nuclei often display incomplete alignment (cohesion) along sister chromatid arms. Sister chromatid cohesion mediated by the multi-subunit cohesin complex is essential for correct chromosome segregation during nuclear divisions and for DNA recombination repair. The cohesin complex consists of the conserved proteins SMC1, SMC3, SCC3, and an alpha-kleisin subunit. Viable homozygous mutants could be selected for the Arabidopsis thaliana alpha-kleisins SYN1, SYN2, and SYN4, which can partially compensate each other. For the kleisin SYN3 and for the single-copy genes SMC1, SMC3, and SCC3, only heterozygous mutants were obtained that displayed between 77% and 97% of the wild-type transcript level. Compared to wild-type nuclei, sister chromatid alignment was significantly decreased along arms in 4C nuclei of the homozygous syn1 and syn4 and even of the heterozygous smc1, smc3, scc3, and syn3 mutants. Knocking out SYN1 and SYN4 additionally impaired sister centromere cohesion. Homozygous mutants of SWITCH1 (required for meiotic sister chromatid alignment) displayed sterility and decreased sister arm alignment. For the cohesin loading complex subunit SCC2, only heterozygous mutants affecting sister centromere alignment were obtained. Defects of the alpha-kleisin SYN4, which impair sister chromatid alignment in 4C differentiated nuclei, do apparently not disturb alignment during prometaphase nor cause aneuploidy in meristematic cells. The syn2, 3, 4 scc3 and swi1 mutants display a high frequency of anaphases with bridges (~10% to >20% compared to 2.6% in wild type). Our results suggest that (a) already a slight reduction of the average transcript level in heterozygous cohesin mutants may cause perturbation of cohesion, at least in some leaf cells at distinct loci; (b) the decreased sister chromatid alignment in cohesin mutants can obviously not fully be compensated by other cohesion mechanisms such as DNA concatenation; (c) some cohesin genes, in addition to cohesion, might have further essential functions (e.g., for genome stability, apparently by facilitating correct recombination repair of double-strand breaks).

journal_name

Chromosoma

journal_title

Chromosoma

authors

Schubert V,Weissleder A,Ali H,Fuchs J,Lermontova I,Meister A,Schubert I

doi

10.1007/s00412-009-0220-x

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

591-605

issue

5

eissn

0009-5915

issn

1432-0886

journal_volume

118

pub_type

杂志文章
  • An elegant miRror: microRNAs in stem cells, developmental timing and cancer.

    abstract::MicroRNAs (miRNAs) were first discovered in genetic screens for regulators of developmental timing in the stem-cell-like seam cell lineage in Caenorhabditis elegans. As members of the heterochronic pathway, the lin-4 and let-7 miRNAs are required in the seam cells for the correct progression of stage-specific events a...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-009-0210-z

    authors: Nimmo RA,Slack FJ

    更新日期:2009-08-01 00:00:00

  • Fluorescent in situ hybridization (FISH) analysis of the relationship between chromosome location and nuclear morphology in human neutrophils.

    abstract::Human neutrophil nuclei typically consist of three of four large heterochromatic lobes joined by thin, DNA-containing filaments. In addition, some lobes exhibit appendages of various sizes and shapes. Classical genetic and cytological studies suggest that some appendages contain specific chromosomes. The studies repor...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050236

    authors: Aquiles Sanchez J,Karni RJ,Wangh LJ

    更新日期:1997-08-01 00:00:00

  • No multistrandedness in mitotic chromosomes of Drosophila melanogaster.

    abstract::Feulgen cytophotometric measurements of neuroblasts in the first and third instar larvae of Drosophila melanogaster reveal the same DNA content for metaphases with chromosomes of different size. The total absorbance of all measured metaphases gives the four-fold value of that of the spermatids. Accordingly there seem ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327078

    authors: van de Flierdt K

    更新日期:1975-01-01 00:00:00

  • A novel translocation event leads to a recombinant stable chromosome with interrupted centromeric domains in rice.

    abstract::Rice (Oryza sativa L.) centromeres are composed of 155-bp satellite repeats (CentO), centromere-specific retrotransposon (CRR), and a variety of other repeats. Previous studies have shown that CentO and CRR elements are both parts of the functional centromere/kinetochore complex. In this study, a naturally occurring k...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-013-0413-1

    authors: Wang G,Li H,Cheng Z,Jin W

    更新日期:2013-08-01 00:00:00

  • Variation in transcriptome size: are we getting the message?

    abstract::The number of RNA molecules per cell (transcriptome size) is highly variable, differing among and within cell types depending on cell size, stage of the cell cycle, ploidy level, age, disease state, and growth condition. Such variation has been observed at the level of total RNA, ribosomal RNA, messenger RNA (mRNA), a...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-014-0496-3

    authors: Coate JE,Doyle JJ

    更新日期:2015-03-01 00:00:00

  • Structure of kinetochore fibers: microtubule continuity and inter-microtubule bridges.

    abstract::To understand how microtubules interact in forming the mitotic apparatus and orienting and moving chromosomes, the precise arrangement of microtubules in kinetochore fibers in Chinese hamster ovary cells was examined. Individual microtubules were traced, using high voltage electron microscopy of serial 0.25 micron sec...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328277

    authors: Witt PL,Ris H,Borisy GG

    更新日期:1981-01-01 00:00:00

  • Simultaneous inhibition of contractile ring and central spindle formation in mammalian cells treated with cytochalasin B.

    abstract::In this work we have used the inhibitor of F-actin polymerisation cytochalasin B (Cyt B) to test the hypothesis that the contractile ring and the central spindle are mutually interdependent structures in mammalian mitotic cells. Double fluorescence staining of alpha-tubulin and F-actin was employed to analyse anaphase...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050332

    authors: Cimini D,Fioravanti D,Tanzarella C,Degrassi F

    更新日期:1998-12-01 00:00:00

  • Chromosome segregation and spindle structure in crane fly spermatocytes following Colcemid treatment.

    abstract::Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00291004

    authors: LaFountain JR Jr

    更新日期:1985-01-01 00:00:00

  • Understanding the birth of rupture-prone and irreparable micronuclei.

    abstract::Micronuclei are extra-nuclear bodies mainly derived from ana-telophase lagging chromosomes/chromatins (LCs) that are not incorporated into primary nuclei at mitotic exit. Unlike primary nuclei, most micronuclei are enclosed by nuclear envelope (NE) that is highly susceptible to spontaneous and irreparable rupture. Rup...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-020-00741-w

    authors: Guo X,Dai X,Wu X,Zhou T,Ni J,Xue J,Wang X

    更新日期:2020-12-01 00:00:00

  • Mechanisms for sister chromatid exchanges and their relation to the production of chromosomal aberrations.

    abstract::By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292776

    authors: Kato H

    更新日期:1977-02-03 00:00:00

  • RNA polymerase II transcription and the functional organization of the mammalian cell nucleus.

    abstract::The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/BF00355315

    authors: Hendzel MJ,Bazett-Jones DP

    更新日期:1995-02-01 00:00:00

  • Isolation and molecular analysis of inv dup(15) and construction of a physical map of a common breakpoint in order to elucidate their mechanism of formation.

    abstract::An inverted duplication of chromosome 15 [inv dup(15)] is the most common supernumerary marker chromosome, comprising approximately 50% of all chromosomes in this class. Structurally, the inv dup(15) is a mirror image with the central axis defining a distal break within either the heterochromatic alpha-satellite array...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120000103

    authors: Wandstrat AE,Schwartz S

    更新日期:2000-11-01 00:00:00

  • Epigenetic profiling of heterochromatic satellite DNA.

    abstract::Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-011-0325-x

    authors: Zakrzewski F,Weisshaar B,Fuchs J,Bannack E,Minoche AE,Dohm JC,Himmelbauer H,Schmidt T

    更新日期:2011-08-01 00:00:00

  • Linking replication stress with heterochromatin formation.

    abstract::The eukaryotic genome can be roughly divided into euchromatin and heterochromatin domains that are structurally and functionally distinct. Heterochromatin is characterized by its high compaction that impedes DNA transactions such as gene transcription, replication, or recombination. Beyond its role in regulating DNA a...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-015-0545-6

    authors: Nikolov I,Taddei A

    更新日期:2016-06-01 00:00:00

  • Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). I. Inter- and intraspecific karyotype diversity.

    abstract::The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293452

    authors: Shaw DD

    更新日期:1976-02-23 00:00:00

  • Haspin: a newly discovered regulator of mitotic chromosome behavior.

    abstract::The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-009-0250-4

    authors: Higgins JM

    更新日期:2010-04-01 00:00:00

  • Anomalous development and differential DNA replication in the X-chromosome of a Drosophila hybrid.

    abstract::Male hybrids of the cross D. azteca X D. athabasca are larger (hybrid giant males) than their parents, whereas hybrid females are of the same size as the parental species. Microspectrophotometric measurements have shown that the larval polytene salivary gland chromosomes of hybrid giant males undergo one more endorepl...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00295210

    authors: Meer B

    更新日期:1976-09-10 00:00:00

  • Incomplete sister chromatid separation of long chromosome arms.

    abstract::Chromosome segregation ensures the equal partitioning of chromosomes at mitosis. However, long chromosome arms may pose a problem for complete sister chromatid separation. In this paper we report on the analysis of cell division in primary cells from field vole Microtus agrestis, a species with 52 chromosomes includin...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-006-0077-1

    authors: Rens W,Torosantucci L,Degrassi F,Ferguson-Smith MA

    更新日期:2006-12-01 00:00:00

  • Cytogenetic replication studies on murine T-cell leukemias with special consideration to chromosome 15.

    abstract::Chromosomal replication was studied by means of the BrdU-Höchst-Giemsa-technique in three minute T-cell leukemias with special regard to chromosome 15. In all the three leukemic cell lines the normally early replicating band 15 E was undetectable whereas late replication in this region is completely unaltered. Further...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288676

    authors: Somssich IE,Spira J,Hameister H,Klein G

    更新日期:1982-01-01 00:00:00

  • Cell cycle dependent chromosomal movement in pre-mitotic human T-lymphocyte nuclei.

    abstract::Fluorescent in situ hybridization with chromosome specific probes was used in conjunction with laser scanning confocal microscopy to assess the three-dimensional distribution of chromosomes in human T-lymphocyte nuclei. Cells in the G1-phase of the cell cycle exhibit a distinctly non-random chromosome organization:cen...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00660315

    authors: Ferguson M,Ward DC

    更新日期:1992-08-01 00:00:00

  • Identification of Porto-1, a new repeated sequence that localises close to the centromere of chromosome 2 of Drosophila melanogaster.

    abstract::We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:

    authors: Coelho PA,Nurminsky D,Hartl D,Sunkel CE

    更新日期:1996-10-01 00:00:00

  • Evolution of Y chromosomal lampbrush loop DNA sequences of Drosophila.

    abstract::The evolutionary conservation of Y chromosomal DNA sequences of Drosophila hydei in different species of the genus Drosophila was studied by in situ hybridization and on genomic DNA blots of restriction enzyme digested DNA. We demonstrated that Y specific DNA sequences, which form major parts of lampbrush loops relate...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328637

    authors: Vogt P,Hennig W,ten Hacken D,Verbost P

    更新日期:1986-01-01 00:00:00

  • "Doubled-haploid" allohexaploid Brassica lines lose fertility and viability and accumulate genetic variation due to genomic instability.

    abstract::Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-019-00720-w

    authors: Mwathi MW,Schiessl SV,Batley J,Mason AS

    更新日期:2019-12-01 00:00:00

  • The diameter of chromatin fibres depends on linker length.

    abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293338

    authors: Alegre C,Subirana JA

    更新日期:1989-06-01 00:00:00

  • RNA polymerase B (or II) in heat induced puffs of Drosophila polytene chromosomes.

    abstract::Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329465

    authors: Greenleaf AL,Plagens U,Jamrich M,Bautz EK

    更新日期:1978-01-16 00:00:00

  • Characterization of macronuclear DNA in five species of ciliates.

    abstract::Macronuclear DNA of four hypotrichous and one holotrichous ciliate species was characterized by biochemical techniques. The renaturation kinetics of the macronuclear DNAs of all five species were similar. Repetitive sequences occur only in an amount below 2%. Although the DNA content of the macronuclei of the species ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286789

    authors: Steinbrück G,Haas I,Hellmer KH,Ammermann D

    更新日期:1981-01-01 00:00:00

  • Contrasting behavior of heterochromatic and euchromatic chromosome portions and pericentric genome separation in pre-bouquet spermatocytes of hybrid mice.

    abstract::The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-014-0479-4

    authors: Scherthan H,Schöfisch K,Dell T,Illner D

    更新日期:2014-12-01 00:00:00

  • Testis-specific beta 2 tubulins are identical in Drosophila melanogaster and D. hydei but differ from the ubiquitous beta 1 tubulin.

    abstract::In Drosophila as in many organisms beta tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the beta 1 and beta 2 tubulins of Drosophila melanogaster and the beta 2 tubulin of D. hydei, and found these insect beta tubulins to be highly conserved and like beta tubulins...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00333989

    authors: Michiels F,Falkenburg D,Müller AM,Hinz U,Otto U,Bellmann R,Glätzer KH,Brand R,Bialojan S,Renkawitz-Pohl R

    更新日期:1987-01-01 00:00:00

  • Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin.

    abstract::In a variety of organisms, euchromatic genes brought into juxtaposition with pericentric heterochromatin show position-effect variegation (PEV), a silencing of gene expression in a subset of the cells in which the gene is normally expressed. Previously, a P-element mobilization screen identified transgenic Drosophila ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050309

    authors: Cryderman DE,Cuaycong MH,Elgin SC,Wallrath LL

    更新日期:1998-11-01 00:00:00

  • Chromosome structure deficiencies in MCPH1 syndrome.

    abstract::Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-015-0512-2

    authors: Arroyo M,Trimborn M,Sánchez A,Hirano T,Neitzel H,Marchal JA

    更新日期:2015-12-01 00:00:00