Incomplete sister chromatid separation of long chromosome arms.

Abstract:

:Chromosome segregation ensures the equal partitioning of chromosomes at mitosis. However, long chromosome arms may pose a problem for complete sister chromatid separation. In this paper we report on the analysis of cell division in primary cells from field vole Microtus agrestis, a species with 52 chromosomes including two giant sex chromosomes. Dual chromosome painting with probes specific for the X and the Y chromosomes showed that these long chromosomes are prone to mis-segregate, producing DNA bridges between daughter nuclei and micronuclei. Analysis of mitotic cells with incomplete chromatid separation showed that reassembly of the nuclear membrane, deposition of INner CENtromere Protein (INCENP)/Aurora B to the spindle midzone and furrow formation occur while the two groups of daughter chromosomes are still connected by sex chromosome arms. Late cytokinetic processes are not efficiently inhibited by the incomplete segregation as in a significant number of cell divisions cytoplasmic abscission proceeds while Aurora B is at the midbody. Live-cell imaging during late mitotic stages also revealed abnormal cell division with persistent sister chromatid connections. We conclude that late mitotic regulatory events do not monitor incomplete sister chromatid separation of the large X and Y chromosomes of Microtus agrestis, leading to defective segregation of these chromosomes. These findings suggest a limit in chromosome arm length for efficient chromosome transmission through mitosis.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Rens W,Torosantucci L,Degrassi F,Ferguson-Smith MA

doi

10.1007/s00412-006-0077-1

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

481-90

issue

6

eissn

0009-5915

issn

1432-0886

journal_volume

115

pub_type

杂志文章
  • FISH analysis of Drosophila melanogaster heterochromatin using BACs and P elements.

    abstract::The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, sever...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-003-0241-9

    authors: Corradini N,Rossi F,Vernì F,Dimitri P

    更新日期:2003-07-01 00:00:00

  • A novel translocation event leads to a recombinant stable chromosome with interrupted centromeric domains in rice.

    abstract::Rice (Oryza sativa L.) centromeres are composed of 155-bp satellite repeats (CentO), centromere-specific retrotransposon (CRR), and a variety of other repeats. Previous studies have shown that CentO and CRR elements are both parts of the functional centromere/kinetochore complex. In this study, a naturally occurring k...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-013-0413-1

    authors: Wang G,Li H,Cheng Z,Jin W

    更新日期:2013-08-01 00:00:00

  • The distribution of sequences complementary to human satellite DNAs I, II and IV in the chromosomes of chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus).

    abstract::Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327453

    authors: Gosden JR,Mitchell AR,Seuanez HN,Gosden CM

    更新日期:1977-09-27 00:00:00

  • Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). II. Variation in the pattern of C-banding.

    abstract::The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293114

    authors: Shaw DD,Webb GC,Wilkinson P

    更新日期:1976-06-30 00:00:00

  • A new role for Drosophila Aurora-A in maintaining chromosome integrity.

    abstract::Aurora-A is a conserved mitotic kinase overexpressed in many types of cancer. Growing evidence shows that Aurora-A plays a crucial role in DNA damage response (DDR) although this aspect has been less characterized. We isolated a new aur-A mutation, named aur-A949, in Drosophila, and we showed that it causes chromosome...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-018-00687-0

    authors: Merigliano C,Mascolo E,Cesta A,Saggio I,Vernì F

    更新日期:2019-03-01 00:00:00

  • Sex chromosome pairing patterns in male mice of novel Sxr genotypes.

    abstract::The influence of the sex-reversal factor (Sxr) on X and Y chromosome pairing was examined by comparing males with "novel" and "standard" Sxr genotypes. The novel Sxr males were exceptional in carrying Sxr on their X rather than their Y chromosome, or homozygously on both their X and Y chromosomes, or on a Y chromosome...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292766

    authors: Tease C,Cattanach BM

    更新日期:1989-03-01 00:00:00

  • 3D genomics imposes evolution of the domain model of eukaryotic genome organization.

    abstract::The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-016-0604-7

    authors: Razin SV,Vassetzky YS

    更新日期:2017-02-01 00:00:00

  • Hanging on to your homolog: the roles of pairing, synapsis and recombination in the maintenance of homolog adhesion.

    abstract::Homologous chromosomes initially undergo weak alignments that bring homologous sequences into register during meiosis. These alignments can be facilitated by two types of mechanisms: interstitial homology searches and telomere-telomere alignments. As prophase (and chromatin compaction) proceeds, these initial pairings...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s004120050407

    authors: Walker MY,Hawley RS

    更新日期:2000-01-01 00:00:00

  • EAST affects the activity of Su(Hw) insulators by two different mechanisms in Drosophila melanogaster.

    abstract::Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-016-0596-3

    authors: Melnikova L,Shapovalov I,Kostyuchenko M,Georgiev P,Golovnin A

    更新日期:2017-03-01 00:00:00

  • Structure of kinetochore fibers: microtubule continuity and inter-microtubule bridges.

    abstract::To understand how microtubules interact in forming the mitotic apparatus and orienting and moving chromosomes, the precise arrangement of microtubules in kinetochore fibers in Chinese hamster ovary cells was examined. Individual microtubules were traced, using high voltage electron microscopy of serial 0.25 micron sec...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328277

    authors: Witt PL,Ris H,Borisy GG

    更新日期:1981-01-01 00:00:00

  • Studies on Macronuclear DNA from Paramecium aurelia.

    abstract::Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329171

    authors: Cummings DJ

    更新日期:1975-12-10 00:00:00

  • Behavior of the chromosome core in mitosis and meiosis.

    abstract::A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292418

    authors: Satya-Prakash KL,Hsu TC,Pathak S

    更新日期:1980-01-01 00:00:00

  • Simultaneous inhibition of contractile ring and central spindle formation in mammalian cells treated with cytochalasin B.

    abstract::In this work we have used the inhibitor of F-actin polymerisation cytochalasin B (Cyt B) to test the hypothesis that the contractile ring and the central spindle are mutually interdependent structures in mammalian mitotic cells. Double fluorescence staining of alpha-tubulin and F-actin was employed to analyse anaphase...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050332

    authors: Cimini D,Fioravanti D,Tanzarella C,Degrassi F

    更新日期:1998-12-01 00:00:00

  • Electron microscopy of membrane-free folded chromosomes from Escherichia coli.

    abstract::Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328479

    authors: Kavenoff R,Bowen BC

    更新日期:1976-12-16 00:00:00

  • Expermental hybridization within the genus Triturus (Urodela: Salamandridae). III. Evidence for crossing-over, true chiasmata and chomosomal homologies in the spermatogenesis of F1 species hybrids, T. cristatus carnifex female X T. marmoratus male.

    abstract::Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00331572

    authors: Mancino G,Ragghianti M,Bucci-Innocenti S

    更新日期:1979-08-10 00:00:00

  • Regulation of splicing by SR proteins and SR protein-specific kinases.

    abstract::Genomic sequencing reveals similar but limited numbers of protein-coding genes in different genomes, which begs the question of how organismal diversities are generated. Alternative pre-mRNA splicing, a widespread phenomenon in higher eukaryotic genomes, is thought to provide a mechanism to increase the complexity of ...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-013-0407-z

    authors: Zhou Z,Fu XD

    更新日期:2013-06-01 00:00:00

  • The diameter of chromatin fibres depends on linker length.

    abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293338

    authors: Alegre C,Subirana JA

    更新日期:1989-06-01 00:00:00

  • Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.

    abstract::A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328531

    authors: Scalenghe F,Buscaglia M,Steinheil C,Crippa M

    更新日期:1978-05-16 00:00:00

  • RNA and macronuclear transcription in the ciliate Stylonychia mytilus.

    abstract::Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286790

    authors: Nock A

    更新日期:1981-01-01 00:00:00

  • Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.

    abstract::Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286046

    authors: Chandley AC,Hotta Y,Stern H

    更新日期:1977-07-08 00:00:00

  • Cloning and characterisation of polymorphic heterochromatic segments of Brachycome dichromosomatica.

    abstract::After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repe...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050430

    authors: Houben A,Wanner G,Hanson L,Verlin D,Leach CR,Timmis JN

    更新日期:2000-06-01 00:00:00

  • Robertsonian polymorphism in the marine gastropod, Nucella lapillus: advances in karyology using rDNA loci and NORs.

    abstract::Previous studies of the Robertsonian polymorphism in the Atlantic dog-whelk, Nucella lapillus (2n = 26-36), have been limited by the inability to identify unequivocally individual chromosomes in the karyotype. This species, as with many other marine invertebrates, has proven largely refractory to the standard (mammali...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352269

    authors: Pascoe PL,Patton SJ,Critcher R,Dixon DR

    更新日期:1996-03-01 00:00:00

  • Transition from somatic to meiotic pairing and progressional changes of the synaptonemal complex in spermatocytes of Aedes aegypti.

    abstract::Aedes aegypti spermatocytes were reconstructed from electron micrographs. The species has tight somatic pairing of the chromosomes, and there are therefore no classical leptotene and zygotene stages, but rather a gradual transition from somatic pairing to meiotic pairing (= pachytene). The term "prepachytene" has been...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329808

    authors: Wandall A,Svendsen A

    更新日期:1985-01-01 00:00:00

  • Macrosatellite epigenetics: the two faces of DXZ4 and D4Z4.

    abstract::Almost half of the human genome consists of repetitive DNA. Understanding what role these elements have in setting up chromatin states that underlie gene and chromosome function in complex genomes is paramount. The function of some types of repetitive DNA is obvious by virtue of their location, such as the alphoid arr...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-009-0233-5

    authors: Chadwick BP

    更新日期:2009-12-01 00:00:00

  • Molecular structure of the rDNA intergenic spacer (IGS) in Triturus: implications for the hypervariability of rDNA loci.

    abstract::Ribosomal DNA (rDNA) variation in the species Triturus vulgaris meridionalis (Amphibia, Urodela) is remarkable because of unusually high intraspecific variability in the number and distribution of ribosomal loci in the karyotype; in addition, portions of the intergenic spacer (IGS) are clustered at chromosomal loci wh...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050253

    authors: De Lucchini S,Andronico F,Nardi I

    更新日期:1997-10-01 00:00:00

  • Ultrastructure of regions containing homologous loci in polytene chromosomes of Drosophila melanogaster and Drosophila subobscura.

    abstract::We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050287

    authors: Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

    更新日期:1998-05-01 00:00:00

  • Structure of the fission yeast centromere cen3: direct analysis of the reiterated inverted region.

    abstract::We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00365153

    authors: Murakami S,Matsumoto T,Niwa O,Yanagida M

    更新日期:1991-12-01 00:00:00

  • Life cycle variation and regulation of macronuclear DNA content in Tetrahymena thermophila.

    abstract::The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327377

    authors: Doerder FP,DeBault LE

    更新日期:1978-10-20 00:00:00

  • Deposition, turnover, and release of CENH3 at Arabidopsis centromeres.

    abstract::The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres vari...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-011-0338-5

    authors: Lermontova I,Rutten T,Schubert I

    更新日期:2011-12-01 00:00:00

  • Synaptonemal complex analysis of X-7 translocations in male mice. I. R3 and R5 quadrivalents.

    abstract::The synaptonemal complexes of surface-spread spermatocytes of mice heterozygous for one of two reciprocal translations (R3 and R5) between the X and chromosome 7 have been examined by light and electron microscopy (EM). The break points of R3 were determined to be at 70% of chromosome 7, as measured from the centromer...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00338486

    authors: Ashley T,Russell LB,Cacheiro NL

    更新日期:1982-01-01 00:00:00