Fluorescent in situ hybridization (FISH) analysis of the relationship between chromosome location and nuclear morphology in human neutrophils.

Abstract:

:Human neutrophil nuclei typically consist of three of four large heterochromatic lobes joined by thin, DNA-containing filaments. In addition, some lobes exhibit appendages of various sizes and shapes. Classical genetic and cytological studies suggest that some appendages contain specific chromosomes. The studies reported here provide the first detailed analysis of the spatial relationship between individual chromosomes and recognizable structures in neutrophil nuclei using fluorescent in situ hybridization. Analysis of DNA sequences in chromosomes 2, 18, X, and Y demonstrate that specific lobes in a population of neutrophil nuclei do not have a fixed chromosome content. This result implies that chromosomes partition randomly among lobes during neutrophil differentiation. However, neutrophil nuclear topography is not entirely fortuitous. For instance, none of the sequences probed in this study mapped to a filament and most centromeres lie in clusters near the nuclear periphery. In addition, one of the X chromosome centromeres in females and the Y chromosome centromere in males consistently associate with specific nuclear appendages found in a subset of neutrophil nuclei. Chromosomes 2 and 18 occupy discrete nd separate territories within individual lobes and neither territory ever extends into a filament. Surprisingly, the sizes of these territories are not proportional to chromosome length, suggesting that individual neutrophil chromosomes vary in their degree of compaction. These results are discussed in the light of models that attempt to explain nuclear morphology in terms of chromosome spatial organization.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Aquiles Sanchez J,Karni RJ,Wangh LJ

doi

10.1007/s004120050236

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

168-77

issue

3

eissn

0009-5915

issn

1432-0886

journal_volume

106

pub_type

杂志文章
  • Saccharomyces cerevisiae cells lacking the homologous pairing protein p175SEP1 arrest at pachytene during meiotic prophase.

    abstract::Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352322

    authors: Bähler J,Hagens G,Holzinger G,Scherthan H,Heyer WD

    更新日期:1994-04-01 00:00:00

  • Cytogenetic replication studies on murine T-cell leukemias with special consideration to chromosome 15.

    abstract::Chromosomal replication was studied by means of the BrdU-Höchst-Giemsa-technique in three minute T-cell leukemias with special regard to chromosome 15. In all the three leukemic cell lines the normally early replicating band 15 E was undetectable whereas late replication in this region is completely unaltered. Further...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288676

    authors: Somssich IE,Spira J,Hameister H,Klein G

    更新日期:1982-01-01 00:00:00

  • Resolving complex chromosome structures during meiosis: versatile deployment of Smc5/6.

    abstract::The Smc5/6 complex, along with cohesin and condensin, is a member of the structural maintenance of chromosome (SMC) family, large ring-like protein complexes that are essential for chromatin structure and function. Thanks to numerous studies of the mitotic cell cycle, Smc5/6 has been implicated to have roles in homolo...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-015-0518-9

    authors: Verver DE,Hwang GH,Jordan PW,Hamer G

    更新日期:2016-03-01 00:00:00

  • Chromosome segregation and spindle structure in crane fly spermatocytes following Colcemid treatment.

    abstract::Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00291004

    authors: LaFountain JR Jr

    更新日期:1985-01-01 00:00:00

  • Nucleolar targeting of coilin is regulated by its hypomethylation state.

    abstract::Coilin, a molecular marker for Cajal bodies (CBs), is a phosphoprotein that contains a cryptic nucleolar localization signal and multiple interacting domains, such as the RG-box. Post-translational symmetrical dimethylation of arginines on the coilin RG-box is required for the recruitment of the survival motor neuron ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-010-0276-7

    authors: Tapia O,Bengoechea R,Berciano MT,Lafarga M

    更新日期:2010-10-01 00:00:00

  • Meiotic pairing and segregation of translocation quadrivalents in yeast.

    abstract::Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050304

    authors: Loidl J,Jin QW,Jantsch M

    更新日期:1998-09-01 00:00:00

  • The centromere composition of multiple repetitive sequences on rice chromosome 5.

    abstract::The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120100148

    authors: Nonomura K,Kurata N

    更新日期:2001-08-01 00:00:00

  • Analysis of DNase 1 sensitivity and methylation of active and inactive X chromosomes of kangaroos (Macropus robustus) by in situ nick translation.

    abstract::The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X c...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00356024

    authors: Loebel DA,Johnston PG

    更新日期:1993-01-01 00:00:00

  • The distribution of sequences complementary to human satellite DNAs I, II and IV in the chromosomes of chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus).

    abstract::Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327453

    authors: Gosden JR,Mitchell AR,Seuanez HN,Gosden CM

    更新日期:1977-09-27 00:00:00

  • New evidence for tandem chromosome fusions in the karyotypic evolution of Asian muntjacs.

    abstract::A clone of highly repetitive DNA, designated C5, was isolated from DNA of female Chinese muntjac cells. The nucleotide sequence of this clone is 80%-85% homologous to that of the satellite IA clone and other highly repetitive DNA clones previously obtained from the Indian muntjac. Using C5 as a probe for in situ hybri...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00360682

    authors: Lin CC,Sasi R,Fan YS,Chen ZQ

    更新日期:1991-10-01 00:00:00

  • Understanding the birth of rupture-prone and irreparable micronuclei.

    abstract::Micronuclei are extra-nuclear bodies mainly derived from ana-telophase lagging chromosomes/chromatins (LCs) that are not incorporated into primary nuclei at mitotic exit. Unlike primary nuclei, most micronuclei are enclosed by nuclear envelope (NE) that is highly susceptible to spontaneous and irreparable rupture. Rup...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-020-00741-w

    authors: Guo X,Dai X,Wu X,Zhou T,Ni J,Xue J,Wang X

    更新日期:2020-12-01 00:00:00

  • Structure, mitotic and meiotic behaviour, and stability of centromere-like elements devoid of chromosome arms in the fly Megaselia scalaris (Phoridae).

    abstract::Minute elements detected in Megaselia scalaris (Phoridae, Diptera) lack chromosome arms but carry centromeres and possess kinetochore microtubules in mitosis as well as in meiosis. These centromere-like elements (CLEs) were present in two geographically independent strains of the fly. This indicates that their origin ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00357059

    authors: Wolf KW,Mertl HG,Traut W

    更新日期:1991-11-01 00:00:00

  • The origin, meiotic behavior, and transmission of a novel minichromosome in Arabidopsis thaliana.

    abstract::A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-005-0045-1

    authors: Murata M,Shibata F,Yokota E

    更新日期:2006-08-01 00:00:00

  • Cloning and characterisation of polymorphic heterochromatic segments of Brachycome dichromosomatica.

    abstract::After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repe...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050430

    authors: Houben A,Wanner G,Hanson L,Verlin D,Leach CR,Timmis JN

    更新日期:2000-06-01 00:00:00

  • Quantitative sequence characterization for repetitive DNA content in the supernumerary chromosome of the migratory locust.

    abstract::Repetitive DNA is a major component in most eukaryotic genomes but is ignored in most genome sequencing projects. Here, we report the quantitative composition in repetitive DNA for a supernumerary (B) chromosome, in the migratory locust (Locusta migratoria), by Illumina sequencing of genomic DNA from B-carrying and B-...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-017-0644-7

    authors: Ruiz-Ruano FJ,Cabrero J,López-León MD,Sánchez A,Camacho JPM

    更新日期:2018-03-01 00:00:00

  • Ultrastructural localization of nucleic acid sequences in Saccharomyces cerevisiae nucleoli.

    abstract::The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352202

    authors: Dvorkin N,Clark MW,Hamkalo BA

    更新日期:1991-09-01 00:00:00

  • Decrease of DNA synthesis in amniotic fluid cells during the middle part of S-phase revealed by differential chromosome staining after incorporation of BrdU.

    abstract::The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293176

    authors: Schempp W,Vogel W

    更新日期:1978-06-28 00:00:00

  • Purification and functional characterization of bovine RP-A in an in vitro SV40 DNA replication system.

    abstract::The single-stranded DNA binding protein RP-A is required in SV40 DNA in vitro replication. The RP-A purified from calf thymus contains 4 polypeptides with molecular weights 70kDa, 53kDa, 32kDa, and 14kDa. The p70 subunit and its proteolysed form p53 are recognized by the monoclonal antibody 70C (Kenny et al. (1990)) a...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF02451786

    authors: Nasheuer HP,von Winkler D,Schneider C,Dornreiter I,Gilbert I,Fanning E

    更新日期:1992-01-01 00:00:00

  • A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.

    abstract::Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-010-0297-2

    authors: Golic MM,Golic KG

    更新日期:2011-04-01 00:00:00

  • Meiotic events at the centromeric heterochromatin: histone H3 phosphorylation, topoisomerase II alpha localization and chromosome condensation.

    abstract::Mechanisms of chromosome condensation and segregation during the first meiotic division are not well understood. Resolution of recombination events to form chiasmata is important, for it is chiasmata that hold homologous chromosomes together for their oppositional orientation on the meiotic metaphase spindle, thus ens...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050393

    authors: Cobb J,Miyaike M,Kikuchi A,Handel MA

    更新日期:1999-12-01 00:00:00

  • Resolving ambiguities in the karyotype of domestic sheep (Ovis aries). II. G-, Q-, and R-banded idiograms, and chromosome-specific molecular markers.

    abstract::Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF02510040

    authors: Ansari HA,Maher DW,Pearce PD,Broad TE

    更新日期:1996-07-01 00:00:00

  • A new look at the nuclear matrix.

    abstract::The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120000077

    authors: Hancock R

    更新日期:2000-07-01 00:00:00

  • Molecular structure of chromatin during sperm differentiation of the dogfish Scyliorhinus caniculus (L.).

    abstract::The molecular structure of chromatin during dogfish spermiogenesis was examined by electron microscopy after the dispersion of nuclei at low ionic strength. In early and late stages of differentiation (round and elongating spermatids), chromatin is globular, although basic nuclear proteins are different from those pre...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292041

    authors: Gusse M,Chevaillier P

    更新日期:1980-01-01 00:00:00

  • A new role for Drosophila Aurora-A in maintaining chromosome integrity.

    abstract::Aurora-A is a conserved mitotic kinase overexpressed in many types of cancer. Growing evidence shows that Aurora-A plays a crucial role in DNA damage response (DDR) although this aspect has been less characterized. We isolated a new aur-A mutation, named aur-A949, in Drosophila, and we showed that it causes chromosome...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-018-00687-0

    authors: Merigliano C,Mascolo E,Cesta A,Saggio I,Vernì F

    更新日期:2019-03-01 00:00:00

  • Proportional polyploidization of 5S RNA genes in the ovary of Drosophila melanogaster mutants containing three 5S RNA gene loci.

    abstract::The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328768

    authors: Renkawitz-Pohl R

    更新日期:1979-01-31 00:00:00

  • Hanging on to your homolog: the roles of pairing, synapsis and recombination in the maintenance of homolog adhesion.

    abstract::Homologous chromosomes initially undergo weak alignments that bring homologous sequences into register during meiosis. These alignments can be facilitated by two types of mechanisms: interstitial homology searches and telomere-telomere alignments. As prophase (and chromatin compaction) proceeds, these initial pairings...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s004120050407

    authors: Walker MY,Hawley RS

    更新日期:2000-01-01 00:00:00

  • A new function for heterochromatin.

    abstract::Meiotic pairing has now been shown to require heterochromatic homology and to be sensitive to repeat number in both male and female Drosophila. The increased pairing ability of repetitive sequences could be one reason that most eukaryotes allow the accumulation of tandem repeated elements. This may well be a reflectio...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/BF00364720

    authors: Irick H

    更新日期:1994-03-01 00:00:00

  • The genetics of dopa decarboxylase in Drosophila melanogaster. IV. The genetics and cytology of the 37B10-37D1 region.

    abstract::Of 204 mutations located in the 8-12 band Df(2L)130 region, 37B9-C1,2;37D1-2, 199 have been assigned to twelve lethal genes and one visible gene (hook). The 13 genes are not evenly distributed. Twelve, (possibly all thirteen) are in the seven band region 37B10-C4 giving a gene-to-band ratio of almost two. Only one gen...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286015

    authors: Wright TR,Beermann W,Marsh JL,Bishop CP,Steward R,Black BC,Tomsett AD,Wright EY

    更新日期:1981-01-01 00:00:00

  • A high resolution study of the DNA replication patterns of chinese hamster chromosomes using sister chromatid differential staining technique.

    abstract::Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00364018

    authors: Crossen PE,Pathak S,Arrighi FE

    更新日期:1975-11-11 00:00:00

  • Microtubule-driven nuclear movements and linear elements as meiosis-specific characteristics of the fission yeasts Schizosaccharomyces versatilis and Schizosaccharomyces pombe.

    abstract::Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japo...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352185

    authors: Svoboda A,Bähler J,Kohli J

    更新日期:1995-11-01 00:00:00