Epigenetic profiling of heterochromatic satellite DNA.

abstract：:This investigation was conducted to determine the relationship between Y chromosome loss and increased micronucleus formation with age. We also investigated the status of kinetochore proteins in the micronuclei. Umbilical cord blood samples were obtained from 18 newborn males, and peripheral blood was obtained from 35...

journal_title：Chromosoma

authors： Nath J,Tucker JD,Hando JC

更新日期：1995-07-01 00:00:00

abstract：:The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was treated with saline-cit...

journal_title：Chromosoma

authors： Comings DE,Avelino E

更新日期：1975-08-11 00:00:00

abstract：:Mitomycin C-induced sister chromatic exchange and quadriradial formation were studied in chromosomes from Muntiacus muntjac fibroblasts and A9, a transformed mouse cell line. We present the first direct cytological evidence for the induction of true somatic recombination involving homologous chromosomes. Analysis of t...

journal_title：Chromosoma

authors： Huttner KM,Ruddle FH

更新日期：1976-06-30 00:00:00

abstract：:Short DNA regions, known to contain replication origins, were isolated from 2 M NaCl resistant nuclear structures of Physarum polycephalum after predigestion with DNase. Regions of 100 bp average length were cloned and sequenced. About 25% of the clones contained direct repeats of 12 to 16 bp and variable base sequenc...

journal_title：Chromosoma

authors： Opstelten RJ,Clement JM,Wanka F

更新日期：1989-12-01 00:00:00

abstract：:The sub-division of S-phase in Syrian hamsters, on the basis of Brd/U/Hoechst 33258/Giemsa banding, has allowed a quantitative comparison of the replication of individual chromosome bands within defined subphases of S. This analysis has shown that in hamsters, as has been reported in humans, there are distinct pattern...

journal_title：Chromosoma

authors： Cawood AH

更新日期：1981-01-01 00:00:00

abstract：:Fluorescent in situ hybridization with chromosome specific probes was used in conjunction with laser scanning confocal microscopy to assess the three-dimensional distribution of chromosomes in human T-lymphocyte nuclei. Cells in the G1-phase of the cell cycle exhibit a distinctly non-random chromosome organization:cen...

journal_title：Chromosoma

authors： Ferguson M,Ward DC

更新日期：1992-08-01 00:00:00

abstract：:In wild-type Drosophila hydei (genotype X/Y) four different primary spermatocyte nuclear glycoproteins, classified as non-Y encoded because of their occurrence in X/O genotypes, were demonstrated to possess a few epitopes that depended on formation of the Y chromosomal giant lampbrush loops threads (th; Mr 55,000 prot...

journal_title：Chromosoma

authors： Tischendorf G,Liebrich W,Trapitz P,Wood G,Schwochau M

更新日期：1989-08-01 00:00:00

abstract：:The Smc5/6 complex, along with cohesin and condensin, is a member of the structural maintenance of chromosome (SMC) family, large ring-like protein complexes that are essential for chromatin structure and function. Thanks to numerous studies of the mitotic cell cycle, Smc5/6 has been implicated to have roles in homolo...

journal_title：Chromosoma

authors： Verver DE,Hwang GH,Jordan PW,Hamer G

更新日期：2016-03-01 00:00:00

abstract：:After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repe...

journal_title：Chromosoma

authors： Houben A,Wanner G,Hanson L,Verlin D,Leach CR,Timmis JN

更新日期：2000-06-01 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...

journal_title：Chromosoma

authors： Schempp W,Vogel W

更新日期：1978-06-28 00:00:00

abstract：:The spindle checkpoint restrains anaphase onset and mitotic exit until all chromosomes are stably attached to the mitotic spindle via their kinetochores. The Tao1 protein kinase was recently reported as a novel spindle checkpoint component. When an siRNA was used to repress Tao1, the essential spindle checkpoint compo...

journal_title：Chromosoma

authors： Westhorpe FG,Diez MA,Gurden MD,Tighe A,Taylor SS

更新日期：2010-08-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...

journal_title：Chromosoma

authors： Scherthan H,Schöfisch K,Dell T,Illner D

更新日期：2014-12-01 00:00:00

abstract：:Human neutrophil nuclei typically consist of three of four large heterochromatic lobes joined by thin, DNA-containing filaments. In addition, some lobes exhibit appendages of various sizes and shapes. Classical genetic and cytological studies suggest that some appendages contain specific chromosomes. The studies repor...

journal_title：Chromosoma

authors： Aquiles Sanchez J,Karni RJ,Wangh LJ

更新日期：1997-08-01 00:00:00

abstract：:Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...

journal_title：Chromosoma

authors： Eichenlaub-Ritter U,Ruthmann A

更新日期：1982-01-01 00:00:00

abstract：:The salivary chromosomes of the l(3)tl mutant of D. melanogaster are considerably thicker and shorter than those of normal larvae. In most nuclei, chromosome shortening is associated with morphological changes of two types. a) The bands lose their distinctive pattern and become loose. The chromosome appears as a granu...

journal_title：Chromosoma

authors： Zhimulev IF,Belyaeva ES,Lychev VA

更新日期：1976-04-21 00:00:00

abstract：:One of the subjects within the meiotic field that has been actively investigated in the recent years is the temporal and functional relationships between meiotic recombination, cohesin loading and synaptonemal complex (SC) assembly. Although the study of meiotic mutants has shed some light, many questions remain to be...

journal_title：Chromosoma

authors： Viera A,Santos JL,Rufas JS

更新日期：2009-06-01 00:00:00

abstract：:The members of the serine-arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle between the nucleus and the cytopl...

journal_title：Chromosoma

authors： Singh OP,Visa N,Wieslander L,Daneholt B

更新日期：2006-12-01 00:00:00

abstract：:The Balbiani rings in the salivary gland polytene chromosomes of Chironomus tentans include the most active structural genes in this organ. Two of them (BR1 and BR2) contain repetitive sequences and are transcribed into giant RNA molecules. On Southern blots of restriction digests, we have identified fragments of geno...

journal_title：Chromosoma

authors： Degelmann A,Hollenberg CP

更新日期：1981-01-01 00:00:00

abstract：:Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...

journal_title：Chromosoma

authors： Mousson F,Ochsenbein F,Mann C

更新日期：2007-04-01 00:00:00

abstract：:The genomic DNA organisation patterns of four sauropsidian species, namely Python reticularis, Caiman crocodilus, Terrapene carolina triungius and Columba livia domestica were investigated by reassociation of short and long DNA fragments, by hyperchromicity measurements of reannealed fragments and by length estimation...

journal_title：Chromosoma

authors： Epplen JT,Diedrich U,Wagenmann M,Schmidtke J,Engel W

更新日期：1979-11-01 00:00:00

abstract：:In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...

journal_title：Chromosoma

authors： Van Hooser AA,Yuh P,Heald R

更新日期：2005-12-01 00:00:00

abstract：:Epigenetic regulation of higher-order chromatin structure controls gene expression and the assembly of chromosomal domains during cell division, differentiation, and development. The proposed "histone code" integrates a complex system of histone modifications and chromosomal proteins that establish and maintain distin...

journal_title：Chromosoma

authors： Lam AL,Pazin DE,Sullivan BA

更新日期：2005-09-01 00:00:00

abstract：:Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded state...

journal_title：Chromosoma

authors： Maksimov DA,Koryakov DE,Belyakin SN

更新日期：2014-06-01 00:00:00

abstract：:The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...

journal_title：Chromosoma

authors： Hendzel MJ,Bazett-Jones DP

更新日期：1995-02-01 00:00:00

abstract：:Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, te...

journal_title：Chromosoma

authors： Wallrath LL,Guntur VP,Rosman LE,Elgin SC

更新日期：1996-04-01 00:00:00

abstract：:The number of RNA molecules per cell (transcriptome size) is highly variable, differing among and within cell types depending on cell size, stage of the cell cycle, ploidy level, age, disease state, and growth condition. Such variation has been observed at the level of total RNA, ribosomal RNA, messenger RNA (mRNA), a...

journal_title：Chromosoma

authors： Coate JE,Doyle JJ

更新日期：2015-03-01 00:00:00

abstract：:We studied the stability of the genomic distribution of six retrotransposon families in long-term and short-term cultures of Drosophila cells. In a subclone derived from Kc cells, no significant rearrangements were detected over an 8 year period. On the contrary, extensive reshuffling and amplification of transposon f...

journal_title：Chromosoma

authors： Junakovic N,Di Franco C,Best-Belpomme M,Echalier G

更新日期：1988-11-01 00:00:00

abstract：:In situ hybridization with 3H 18S and 28S ribosomal RNA from Xenopus laevis has been used to study the distribution of DNA sequences coding for these RNAs (the nucleolus organizing regions) in the genomes of six mammals. Several patterns of distribution have been found: 1) A single major site (rat kangaroo, Seba's fru...

journal_title：Chromosoma

authors： Hsu TC,Spirito SE,Pardue ML

更新日期：1975-11-20 00:00:00