Abstract:
:The salivary chromosomes of the l(3)tl mutant of D. melanogaster are considerably thicker and shorter than those of normal larvae. In most nuclei, chromosome shortening is associated with morphological changes of two types. a) The bands lose their distinctive pattern and become loose. The chromosome appears as a granular mass. In extreme cases pompon-like chromosomes arise. Most frequently male X-chromosomes undergo such changes and begin to shorten as early as in the middle of third larval instar. "Pompon" transformation is not associated with a change in the relative intensity of RNA synthesis: the ratio of silver grain number over the male X-chromosome to that over regions 61A-63F is the same in pompon-like l(3)tl chromosomes and in the male and female X-chromosomes of the normal lines. b) Shortened chromosomes occassionally retain distinct band organization and, in these cases, chromosome shortening is observed to be due to the condensation of the chromatin of many puffs and interbands resulting in the fusion of a large number of bands into "new" chromatin blocks. In regions of fused bands, transcriptional activity is decreased as compared with regions where this union does not occur. The chromosomes of l(3)tl larvae lack ecdysone-stimulated puffs and other prominent puffs. In 144-192 hour larvae, puffs can be induced by ecdysone and until 384 hours by temperature shock. The capacity of puff induction decreases with larval age.
journal_name
Chromosomajournal_title
Chromosomaauthors
Zhimulev IF,Belyaeva ES,Lychev VAdoi
10.1007/BF01798344subject
Has Abstractpub_date
1976-04-21 00:00:00pages
121-36issue
2eissn
0009-5915issn
1432-0886journal_volume
55pub_type
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