Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster.

abstract：:A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...

journal_title：Chromosoma

authors： Murata M,Shibata F,Yokota E

更新日期：2006-08-01 00:00:00

abstract：:BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolut...

journal_title：Chromosoma

authors： Latt SA,Willard HF,Gerald PS

更新日期：1976-08-17 00:00:00

abstract：:Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...

journal_title：Chromosoma

authors： Mwathi MW,Schiessl SV,Batley J,Mason AS

更新日期：2019-12-01 00:00:00

abstract：:The DNA coding for 28 S and 18 S ribosomal RNA, including the spacer regions, has been isolated from calf (Bos taurus) thymus gland. The method used included shearing of the total DNA to a highly homogeneous size population, selective heat denaturation and S1 nuclease treatment to remove single stranded DNA. Repeated ...

journal_title：Chromosoma

authors： Blin N,Stephenson EC,Stafford DW

更新日期：1976-10-12 00:00:00

abstract：:We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is charac...

journal_title：Chromosoma

authors： Jantsch M,Hamilton B,Mayr B,Schweizer D

更新日期：1990-09-01 00:00:00

abstract：:Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japo...

journal_title：Chromosoma

authors： Svoboda A,Bähler J,Kohli J

更新日期：1995-11-01 00:00:00

abstract：:The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...

journal_title：Chromosoma

authors： Ohno M,Fukagawa T,Lee JS,Ikemura T

更新日期：2002-09-01 00:00:00

abstract：:The appearance of RNP droplets associated with specific regions of polytene chromosomes from Chironomus larvae has been studied, systematically by light microscopy using glutarladehyde fixation and lactic acid squashing of the salivary gland. During the fourth larval instar the highest frequency of nuclei which contai...

journal_title：Chromosoma

authors： Stockert JC,Díez JL

更新日期：1979-09-01 00:00:00

abstract：:Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

journal_title：Chromosoma

authors： Faisal I,Kauppi L

更新日期：2016-06-01 00:00:00

abstract：:Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

journal_title：Chromosoma

authors： Loidl J,Jin QW,Jantsch M

更新日期：1998-09-01 00:00:00

abstract：:The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...

journal_title：Chromosoma

authors： Hancock R

更新日期：2000-07-01 00:00:00

abstract：:The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the fusion orientations of c...

journal_title：Chromosoma

authors： Chi JX,Huang L,Nie W,Wang J,Su B,Yang F

更新日期：2005-08-01 00:00:00

abstract：:Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observ...

journal_title：Chromosoma

authors： Cummings DJ

更新日期：1975-12-10 00:00:00

abstract：:Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-...

journal_title：Chromosoma

authors： Zakrzewski F,Weisshaar B,Fuchs J,Bannack E,Minoche AE,Dohm JC,Himmelbauer H,Schmidt T

更新日期：2011-08-01 00:00:00

abstract：:Compelling evidence has been obtained in favour of the idea that the nuclear surface of higher plant cells is a microtubule-nucleating and/or organizing site (MTOC), in the absence of defined centrosomes. How these plant MTOC proteins are redistributed and function during the progression of the cell cycle remains enti...

journal_title：Chromosoma

authors： Schmit AC,Stoppin V,Chevrier V,Job D,Lambert AM

更新日期：1994-09-01 00:00:00

abstract：:In budding yeast, mitotic DNA replication initiates at sequence-specific replication origins, the prototype for which is ARS1. Initiation serves as the primary control point for mitotic DNA replication, and is catalyzed by the Cdc7 protein kinase. In contrast, premeiotic DNA replication apparently does not require Cdc...

journal_title：Chromosoma

authors： Hollingsworth RE Jr,Sclafani RA

更新日期：1993-06-01 00:00:00

abstract：:Thirty P1 clones from the X chromosome (Muller's A element) of Drosophila melanogaster were cross-hybridized in situ to Drosophila subobscura and Drosophila pseudoobscura polytene chromosomes. An additional recombinant phage lambda Dsuby was also used as a marker. Twenty-three (77%) of the P1 clones gave positive hybr...

journal_title：Chromosoma

authors： Segarra C,Lozovskaya ER,Ribó G,Aguadé M,Hartl DL

更新日期：1995-11-01 00:00:00

abstract：:The distribution patterns of chromosomal proteins from Drosophila can be observed by immunofluorescent staining of the polytene chromosomes from larval salivary glands. We have purified a non-histone chromosomal protein of Mr = 69,000 molecular weight which has a high affinity for DNA with little sequence specificity....

journal_title：Chromosoma

authors： Fleischmann G,Filipski R,Elgin SC

更新日期：1987-01-01 00:00:00

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of cent...

journal_title：Chromosoma

authors： Zalensky AO,Allen MJ,Kobayashi A,Zalenskaya IA,Balhórn R,Bradbury EM

更新日期：1995-05-01 00:00:00

abstract：:Chromosome segregation ensures the equal partitioning of chromosomes at mitosis. However, long chromosome arms may pose a problem for complete sister chromatid separation. In this paper we report on the analysis of cell division in primary cells from field vole Microtus agrestis, a species with 52 chromosomes includin...

journal_title：Chromosoma

authors： Rens W,Torosantucci L,Degrassi F,Ferguson-Smith MA

更新日期：2006-12-01 00:00:00

abstract：:Epigenetic regulation of higher-order chromatin structure controls gene expression and the assembly of chromosomal domains during cell division, differentiation, and development. The proposed "histone code" integrates a complex system of histone modifications and chromosomal proteins that establish and maintain distin...

journal_title：Chromosoma

authors： Lam AL,Pazin DE,Sullivan BA

更新日期：2005-09-01 00:00:00

abstract：:Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...

journal_title：Chromosoma

authors： Yamamoto Y,Gerbi SA

更新日期：2018-12-01 00:00:00

abstract：:We probed the complex between oriC and DnaA protein using two types of mutants in oriC. Base changes in the DnaA binding sites, DnaA boxes, had little effect on origin function. Mutations which change the distance between DnaA boxes R3 and R4, on the other hand, inactivated oriC unless the mutation deleted or inserted...

journal_title：Chromosoma

authors： Messer W,Hartmann-Kühlein H,Langer U,Mahlow E,Roth A,Schaper S,Urmoneit B,Woelker B

更新日期：1992-01-01 00:00:00

abstract：:Drosophila melanogaster was transformed with an 18 kb fragment of the C3 DNA puff of Rhynchosciara americana, including the C3-22 gene and the origins of replication that direct amplification. Different tissues and developmental stages of five independent transgenic lines were analyzed by quantitative Southern blot hy...

journal_title：Chromosoma

authors： Soares MA,Monesi N,Basso LR Jr,Stocker AJ,Paçó-Larson ML,Lara FJ

更新日期：2003-10-01 00:00:00

abstract：:Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...

journal_title：Chromosoma

authors： Howell WM,Hsu TC

更新日期：1979-06-21 00:00:00

abstract：:Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...

journal_title：Chromosoma

authors： Melnikova L,Shapovalov I,Kostyuchenko M,Georgiev P,Golovnin A

更新日期：2017-03-01 00:00:00

abstract：:We have sequenced and analyzed 8.3 kb of sequence adjacent and distal to the human ribosomal DNA (rDNA); this distal sequence connects to the rDNA cluster just 4 kb upstream of the first promoter and is shared among the acrocentric chromosomes and, at least in part, it is also present in other primates. The sequence d...

journal_title：Chromosoma

authors： Gonzalez IL,Sylvester JE

更新日期：1997-06-01 00:00:00

abstract：:Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...

journal_title：Chromosoma

authors： Cahoon CK,Libuda DE

更新日期：2019-09-01 00:00:00

abstract：:We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...

journal_title：Chromosoma

authors： Kress H,Meyerowitz EM,Davidson N

更新日期：1985-01-01 00:00:00