Isolation and some properties of a mammalian ribosomal DNA.

abstract：:Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces ...

journal_title：Chromosoma

authors： Foulk MS,Waggener JM,Johnson JM,Yamamoto Y,Liew GM,Urnov FD,Young Y,Lee G,Smith HS,Gerbi SA

更新日期：2013-03-01 00:00:00

abstract：:Normal lepidopteran males produce two kinds of spermatozoa: nucleate (eupyrene) and anucleate (apyrene). Eupyrene spermatozoa have the usual type of elongate nuclei. But in apyrene spermatids, the nuclei never elongate and the chromatin remains in a telophase-like condition until enucleation occurs. The study of the d...

journal_title：Chromosoma

authors： Friedländer M,Hauschteck-Jungen E

更新日期：1982-01-01 00:00:00

abstract：:In situ hybridization of labeled DNA of four mobile dispersed genetic elements (mdg), isolated from D. melanogaster and C. virilis genomes, with polytene chromosomes of the larvae of several Drosophila species has been carried out. The data show that the mdg elements exhibit a high degree of species specificity. The s...

journal_title：Chromosoma

authors： Evgen'ev MB,Yenikolopov GN,Peunova NI,Ilyin YV

更新日期：1982-01-01 00:00:00

abstract：:Among his many contributions to the field of chromosome structure and dynamics, J. Herbert Taylor showed that eukaryotic cells have many more potential replication origins than they use, which they can recruit when replication forks are slowed to complete S-phase in a timely fashion. Thirty years later, his findings r...

journal_title：Chromosoma

authors： Gilbert DM

更新日期：2007-08-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

journal_title：Chromosoma

authors： Coelho PA,Nurminsky D,Hartl D,Sunkel CE

更新日期：1996-10-01 00:00:00

abstract：:Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interesting...

journal_title：Chromosoma

authors： Xin H,Zhang T,Han Y,Wu Y,Shi J,Xi M,Jiang J

更新日期：2018-09-01 00:00:00

abstract：:Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...

journal_title：Chromosoma

authors： Morris VB

更新日期：1977-03-16 00:00:00

abstract：:Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded state...

journal_title：Chromosoma

authors： Maksimov DA,Koryakov DE,Belyakin SN

更新日期：2014-06-01 00:00:00

abstract：:Studies using human and mouse cells have revealed some changes to non-telomeric chromatin and gene expression in response to abnormally short telomeres. To investigate this further, we studied the effect of inheriting shorter telomeres on transcription and genetic stability at non-telomeric sites in the mouse. Using m...

journal_title：Chromosoma

authors： Roberts AR,Huang E,Jones L,Daxinger L,Chong S,Whitelaw E

更新日期：2013-12-01 00:00:00

abstract：:The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...

journal_title：Chromosoma

authors： Palmer DK,O'Day K,Margolis RL

更新日期：1990-12-01 00:00:00

abstract：:Antibodies against human Rad51 protein were used to examine the distribution of Rad51 on meiotic chromatin in mouse spermatocytes and oocytes as well as chicken oocytes during sequential stages of meiosis. We observed the following dynamic changes in distribution of Rad51 during meiosis: (1) in early leptotene nuclei ...

journal_title：Chromosoma

authors： Ashley T,Plug AW,Xu J,Solari AJ,Reddy G,Golub EI,Ward DC

更新日期：1995-10-01 00:00:00

abstract：:BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolut...

journal_title：Chromosoma

authors： Latt SA,Willard HF,Gerald PS

更新日期：1976-08-17 00:00:00

abstract：:We describe a novel rearranged human Y chromosome consisting of an inverted duplication of the long arm heterochromatin and a small amount of euchromatin: rea(Y)(qter-q11.2::q11.2-qter). The normal centromere has been deleted and a neocentromere containing CENP-A, -C, -E and Mad2 but not CENP-B has formed close to the...

journal_title：Chromosoma

authors： Floridia G,Gimelli G,Zuffardi O,Earnshaw WC,Warburton PE,Tyler-Smith C

更新日期：2000-01-01 00:00:00

abstract：:An unstable minichromosome comprising part of the short arm of chromosome 10 of maize was recovered from an oil yellow variegated plant as a consequence of gamma irradiation of pollen. The cytological and gene dosage observations are consistent with the minichromosome being a partial isochromosome, which lags at mitot...

journal_title：Chromosoma

authors： Brock RD,Pryor AJ

更新日期：1996-06-01 00:00:00

abstract：:We have sequenced and analyzed 8.3 kb of sequence adjacent and distal to the human ribosomal DNA (rDNA); this distal sequence connects to the rDNA cluster just 4 kb upstream of the first promoter and is shared among the acrocentric chromosomes and, at least in part, it is also present in other primates. The sequence d...

journal_title：Chromosoma

authors： Gonzalez IL,Sylvester JE

更新日期：1997-06-01 00:00:00

abstract：:An inverted duplication of chromosome 15 [inv dup(15)] is the most common supernumerary marker chromosome, comprising approximately 50% of all chromosomes in this class. Structurally, the inv dup(15) is a mirror image with the central axis defining a distal break within either the heterochromatic alpha-satellite array...

journal_title：Chromosoma

authors： Wandstrat AE,Schwartz S

更新日期：2000-11-01 00:00:00

abstract：:The Smc5/6 complex, along with cohesin and condensin, is a member of the structural maintenance of chromosome (SMC) family, large ring-like protein complexes that are essential for chromatin structure and function. Thanks to numerous studies of the mitotic cell cycle, Smc5/6 has been implicated to have roles in homolo...

journal_title：Chromosoma

authors： Verver DE,Hwang GH,Jordan PW,Hamer G

更新日期：2016-03-01 00:00:00

abstract：:Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...

journal_title：Chromosoma

authors： Mwathi MW,Schiessl SV,Batley J,Mason AS

更新日期：2019-12-01 00:00:00

abstract：:Meiotic pairing has now been shown to require heterochromatic homology and to be sensitive to repeat number in both male and female Drosophila. The increased pairing ability of repetitive sequences could be one reason that most eukaryotes allow the accumulation of tandem repeated elements. This may well be a reflectio...

journal_title：Chromosoma

authors： Irick H

更新日期：1994-03-01 00:00:00

abstract：:We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...

journal_title：Chromosoma

authors： Kress H,Meyerowitz EM,Davidson N

更新日期：1985-01-01 00:00:00

abstract：:Using BrdU/Hoechst 33258/Giemsa methods for detecting replicating chromosome bands, a method is described by which the DNA synthesis phase may be sub-divided on the basis of distinctive patterns displayed by certain chromosomes.--Applied to asynchronous populations successively sampled through one cell cycle, cells in...

journal_title：Chromosoma

authors： Savage JR,Bhunya SP

更新日期：1980-01-01 00:00:00

abstract：:Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...

journal_title：Chromosoma

authors： Turner BM,Keohane A

更新日期：1987-01-01 00:00:00

abstract：:Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...

journal_title：Chromosoma

authors： Rizzo PJ,Burghardt RC

更新日期：1980-01-01 00:00:00

abstract：:Almost half of the human genome consists of repetitive DNA. Understanding what role these elements have in setting up chromatin states that underlie gene and chromosome function in complex genomes is paramount. The function of some types of repetitive DNA is obvious by virtue of their location, such as the alphoid arr...

journal_title：Chromosoma

authors： Chadwick BP

更新日期：2009-12-01 00:00:00

abstract：:Histone modifications regulate key processes of eukaryotic genomes. Misregulation of the enzymes that place these modifications can lead to disease. An example of this is DOT1L, the enzyme that can mono-, di-, and trimethylate the nucleosome core on lysine 79 of histone H3 (H3K79). DOT1L plays a role in development an...

journal_title：Chromosoma

authors： Vlaming H,van Leeuwen F

更新日期：2016-09-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...

journal_title：Chromosoma

authors： Capozzi O,Archidiacono N,Lorusso N,Stanyon R,Rocchi M

更新日期：2016-09-01 00:00:00

abstract：:Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM,...

journal_title：Chromosoma

authors： Swanton MT,Heumann JM,Prescott DM

更新日期：1980-01-01 00:00:00

abstract：:Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japo...

journal_title：Chromosoma

authors： Svoboda A,Bähler J,Kohli J

更新日期：1995-11-01 00:00:00

abstract：:The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...

journal_title：Chromosoma

authors： Zacharias H

更新日期：1984-01-01 00:00:00

abstract：:Metaphase chromosomes prepared from colcemid-treated mouse L929 cells by non-ionic detergent lysis exhibit distinct heterochromatic centromere regions and associated kinetochores when viewed by whole mount electron microscopy. Deoxyribonuclease I treatment of these chromosomes results in the preferential digestion of ...

journal_title：Chromosoma

authors： Rattner JB,Krystal G,Hamkalo BA

更新日期：1978-04-25 00:00:00