Isolation and characterization of the ecdysone receptor and its heterodimeric partner ultraspiracle through development in Sciara coprophila.

Abstract:

:Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces DNA amplification in Sciara, and the amplification origin of DNA puff II/9A contains a putative binding site for the ecdysone receptor (EcR). We report here the isolation, cloning, and characterizing of two ecdysone receptor isoforms in Sciara (ScEcR-A and ScEcR-B) and the heterodimeric partner, ultraspiracle (ScUSP). ScEcR-A is the predominant isoform in larval tissues and ScEcR-B in adult tissues, contrary to the pattern in Drosophila. Moreover, ScEcR-A is produced at amplification but is absent just prior. We discuss these results in relation to the model of ecdysone regulation of DNA amplification.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Foulk MS,Waggener JM,Johnson JM,Yamamoto Y,Liew GM,Urnov FD,Young Y,Lee G,Smith HS,Gerbi SA

doi

10.1007/s00412-012-0395-4

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

103-19

issue

1-2

eissn

0009-5915

issn

1432-0886

journal_volume

122

pub_type

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