Abstract:
:Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces DNA amplification in Sciara, and the amplification origin of DNA puff II/9A contains a putative binding site for the ecdysone receptor (EcR). We report here the isolation, cloning, and characterizing of two ecdysone receptor isoforms in Sciara (ScEcR-A and ScEcR-B) and the heterodimeric partner, ultraspiracle (ScUSP). ScEcR-A is the predominant isoform in larval tissues and ScEcR-B in adult tissues, contrary to the pattern in Drosophila. Moreover, ScEcR-A is produced at amplification but is absent just prior. We discuss these results in relation to the model of ecdysone regulation of DNA amplification.
journal_name
Chromosomajournal_title
Chromosomaauthors
Foulk MS,Waggener JM,Johnson JM,Yamamoto Y,Liew GM,Urnov FD,Young Y,Lee G,Smith HS,Gerbi SAdoi
10.1007/s00412-012-0395-4subject
Has Abstractpub_date
2013-03-01 00:00:00pages
103-19issue
1-2eissn
0009-5915issn
1432-0886journal_volume
122pub_type
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