Resolving complex chromosome structures during meiosis: versatile deployment of Smc5/6.

abstract：:One of the subjects within the meiotic field that has been actively investigated in the recent years is the temporal and functional relationships between meiotic recombination, cohesin loading and synaptonemal complex (SC) assembly. Although the study of meiotic mutants has shed some light, many questions remain to be...

journal_title：Chromosoma

authors： Viera A,Santos JL,Rufas JS

更新日期：2009-06-01 00:00:00

abstract：:High resolution patterns of DNA replications in human lymphocyte chromosomes during early and late S-phases were studied by means of the BrdU-Hoechst-Giemsa technique. The late replicating bands were found to be identical with highly detailed G-bands. Between early replicating bands and R-bands subtile differences wer...

journal_title：Chromosoma

authors： Meer B,Hameister H,Cerrillo M

更新日期：1981-01-01 00:00:00

abstract：:Nucleolar matrix structures were obtained under different extraction conditions from highly purified isolated nucleoli. Their ultrastructural appearance, protein composition and capacity to bind rDNA preferentially were studied in a model binding system. A region spanning approximately 25 kb in the rat ribosomal gene ...

journal_title：Chromosoma

authors： Stephanova E,Stancheva R,Avramova Z

更新日期：1993-03-01 00:00:00

abstract：:Evidence is presented that chromosome ends are attached to the nuclear envelope prior to the formation of axial cores during early meiotic prophase in the grasshopper Brachystola magna. The attachment sites of distal and proximal chromosome ends are clustered in a small region of the inner nuclear envelope resulting i...

journal_title：Chromosoma

authors： Church K

更新日期：1976-11-29 00:00:00

abstract：:Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM,...

journal_title：Chromosoma

authors： Swanton MT,Heumann JM,Prescott DM

更新日期：1980-01-01 00:00:00

abstract：:A higher order organization of the centromeres in the form of clustering of these DNA loci has been observed in many organisms. While centromere clustering is biologically significant to achieve faithful chromosome segregation, the underlying molecular mechanism is yet to be fully understood. In budding yeast, a kinet...

journal_title：Chromosoma

authors： Mittal P,Chavan A,Trakroo D,Shah S,Ghosh SK

更新日期：2019-06-01 00:00:00

abstract：:The kinetochore binds an evolutionarily conserved set of checkpoint proteins that function to monitor whether chromosomes have aligned properly at the spindle equator. Human cells contain two related protein kinases, hBUB1 and hBUBR1, that appear to have evolved from a single ancestral BUB1 gene. We generated hBUB1- a...

journal_title：Chromosoma

authors： Jablonski SA,Chan GK,Cooke CA,Earnshaw WC,Yen TJ

更新日期：1998-12-01 00:00:00

abstract：:Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...

journal_title：Chromosoma

authors： Petcherskaia M,McGuire JM,Pherson JM,Kirk KE

更新日期：2003-04-01 00:00:00

abstract：:The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

journal_title：Chromosoma

authors： Doerder FP,DeBault LE

更新日期：1978-10-20 00:00:00

abstract：:A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...

journal_title：Chromosoma

authors： Murata M,Shibata F,Yokota E

更新日期：2006-08-01 00:00:00

abstract：:Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...

journal_title：Chromosoma

authors： Dowsett AP

更新日期：1983-01-01 00:00:00

abstract：:The XY bivalent contained within the sex vesicle of chromosomally normal male mice shows virtually no RNA transcriptional activity as measured by autoradiography during the prophase of meiosis. Tertiary trisomic males, derived from the T 70 H and T 31 H reciprocal translocations, include the extra small marker chromos...

journal_title：Chromosoma

authors： Speed RM

更新日期：1986-01-01 00:00:00

abstract：:We studied the stability of the genomic distribution of six retrotransposon families in long-term and short-term cultures of Drosophila cells. In a subclone derived from Kc cells, no significant rearrangements were detected over an 8 year period. On the contrary, extensive reshuffling and amplification of transposon f...

journal_title：Chromosoma

authors： Junakovic N,Di Franco C,Best-Belpomme M,Echalier G

更新日期：1988-11-01 00:00:00

abstract：:The chromatin protein Polycomb (PC) is necessary for keeping homeotic genes repressed in a permanent and heritable manner. PC is part of a large multimeric complex (PcG proteins) involved in generating silenced chromatin domains at target genes, thus preventing their inappropriate expression. In order to assess the in...

journal_title：Chromosoma

authors： Dietzel S,Niemann H,Brückner B,Maurange C,Paro R

更新日期：1999-05-01 00:00:00

abstract：:The DNA of eukaryotic cells is organized in a complex with proteins, either as interphase chromatin or mitotic chromosomes. Nucleosomes, the structural subunits of chromatin, have long been considered as static structures, incompatible with processes occurring in chromatin. During the past few years it has become evid...

journal_title：Chromosoma

authors： Loidl P

更新日期：1994-12-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) and Aurora A play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Here we show that the functions of these kinases during early mitosis are coordinated through Bora, a partner of Aurora A first identified in Drosophila. Depletion of human...

journal_title：Chromosoma

authors： Chan EH,Santamaria A,Silljé HH,Nigg EA

更新日期：2008-10-01 00:00:00

abstract：:Neuroblast chromosomes of 16 Drosophila melanogaster laboratory stocks (15 wild type and 1 carrying the mutant vermillion) were carefully analyzed for Q-banding patterns and morphological characteristics, in all the mitotic phases. Two forms of intraspecific heterochromatin variations, involving three types of chromos...

journal_title：Chromosoma

authors： Halfer C

更新日期：1981-01-01 00:00:00

abstract：:We have characterized two post-translational histone modifications in Caenorhabditis elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional a...

journal_title：Chromosoma

authors： Gu SG,Fire A

更新日期：2010-02-01 00:00:00

abstract：:Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...

journal_title：Chromosoma

authors： Arroyo M,Trimborn M,Sánchez A,Hirano T,Neitzel H,Marchal JA

更新日期：2015-12-01 00:00:00

abstract：:Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

journal_title：Chromosoma

authors： Faisal I,Kauppi L

更新日期：2016-06-01 00:00:00

abstract：:Metaphase chromosomes from the Chinese hamster cell line M3-1 were separated by means of a flow sorter. Two chromosome fractions were used for this study: A, which consisted of 95% pure chromosome no. 1, and B, which was 90% pure chromosome no. 2. The DNA of 10(6) chromosomes of each type was purified, and a 125I-cRNA...

journal_title：Chromosoma

authors： Sawin VL,Rowley JD,Carrano AV

更新日期：1979-01-31 00:00:00

abstract：:Chromosome segregation ensures the equal partitioning of chromosomes at mitosis. However, long chromosome arms may pose a problem for complete sister chromatid separation. In this paper we report on the analysis of cell division in primary cells from field vole Microtus agrestis, a species with 52 chromosomes includin...

journal_title：Chromosoma

authors： Rens W,Torosantucci L,Degrassi F,Ferguson-Smith MA

更新日期：2006-12-01 00:00:00

abstract：:The sub-division of S-phase in Syrian hamsters, on the basis of Brd/U/Hoechst 33258/Giemsa banding, has allowed a quantitative comparison of the replication of individual chromosome bands within defined subphases of S. This analysis has shown that in hamsters, as has been reported in humans, there are distinct pattern...

journal_title：Chromosoma

authors： Cawood AH

更新日期：1981-01-01 00:00:00

abstract：:A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was ...

journal_title：Chromosoma

authors： Harauz G,Borland L,Bahr GF,Zeitler E,van Heel M

更新日期：1987-01-01 00:00:00

abstract：:Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...

journal_title：Chromosoma

authors： Turner BM,Keohane A

更新日期：1987-01-01 00:00:00

abstract：:Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japo...

journal_title：Chromosoma

authors： Svoboda A,Bähler J,Kohli J

更新日期：1995-11-01 00:00:00

abstract：:The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Sat...

journal_title：Chromosoma

authors： Gisselsson D,Shao C,Tuck-Muller CM,Sogorovic S,Pålsson E,Smeets D,Ehrlich M

更新日期：2005-07-01 00:00:00

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00