Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast.

Abstract:

:A higher order organization of the centromeres in the form of clustering of these DNA loci has been observed in many organisms. While centromere clustering is biologically significant to achieve faithful chromosome segregation, the underlying molecular mechanism is yet to be fully understood. In budding yeast, a kinetochore-associated protein Slk19 is shown to have a role in clustering in association with the microtubules whereas removal of either Slk19 or microtubules alone does not have any effect on the centromere clustering. Furthermore, Slk19 is non-essential for growth and becomes cleaved during anaphase whereas clustering being an essential event occurs throughout the cell cycle. Hence, we searched for an additional factor involved in the clustering and since the integrity of the kinetochore complex is shown to be crucial for centromere clustering, we restricted our search within the complex. We observed that the outermost kinetochore protein Dam1 promotes centromere clustering through stabilization of the kinetochore integrity. While in the absence of Dam1 we failed to detect Slk19 at the centromere, on the other hand, we found almost no Dam1 at the centromere in the absence of Slk19 and microtubules suggesting interdependency between these two pathways. Strikingly, we observed that overexpression of Dam1 or Slk19 could restore the centromere clustering largely in the cells devoid of Slk19 and microtubules or Dam1, respectively. Thus, we propose that in budding yeast, centromere clustering is achieved at least by two parallel pathways, through Dam1 and another via Slk19, in concert with the microtubules suggesting that having a dual mechanism may be crucial for ensuring microtubule capture by the point centromeres where each attaches to only one microtubule.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Mittal P,Chavan A,Trakroo D,Shah S,Ghosh SK

doi

10.1007/s00412-019-00694-9

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

133-148

issue

2

eissn

0009-5915

issn

1432-0886

pii

10.1007/s00412-019-00694-9

journal_volume

128

pub_type

杂志文章
  • Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster.

    abstract::The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-019-00703-x

    authors: Sidorenko DS,Sidorenko IA,Zykova TY,Goncharov FP,Larsson J,Zhimulev IF

    更新日期:2019-06-01 00:00:00

  • The upstreams and downstreams of H3K79 methylation by DOT1L.

    abstract::Histone modifications regulate key processes of eukaryotic genomes. Misregulation of the enzymes that place these modifications can lead to disease. An example of this is DOT1L, the enzyme that can mono-, di-, and trimethylate the nucleosome core on lysine 79 of histone H3 (H3K79). DOT1L plays a role in development an...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-015-0570-5

    authors: Vlaming H,van Leeuwen F

    更新日期:2016-09-01 00:00:00

  • Chromosomal arrangement of heat shock locus 2-48B in Drosophila hydei.

    abstract::cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00294968

    authors: Peters FP,Grond CJ,Sondermeijer PJ,Lubsen NH

    更新日期:1982-01-01 00:00:00

  • Cohesin gene defects may impair sister chromatid alignment and genome stability in Arabidopsis thaliana.

    abstract::In contrast to yeast, plant interphase nuclei often display incomplete alignment (cohesion) along sister chromatid arms. Sister chromatid cohesion mediated by the multi-subunit cohesin complex is essential for correct chromosome segregation during nuclear divisions and for DNA recombination repair. The cohesin complex...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-009-0220-x

    authors: Schubert V,Weissleder A,Ali H,Fuchs J,Lermontova I,Meister A,Schubert I

    更新日期:2009-10-01 00:00:00

  • Ultrastructural localization of nucleic acid sequences in Saccharomyces cerevisiae nucleoli.

    abstract::The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352202

    authors: Dvorkin N,Clark MW,Hamkalo BA

    更新日期:1991-09-01 00:00:00

  • Yeast pre-meiotic DNA replication utilizes mitotic origin ARS1 independently of CDC7 function.

    abstract::In budding yeast, mitotic DNA replication initiates at sequence-specific replication origins, the prototype for which is ARS1. Initiation serves as the primary control point for mitotic DNA replication, and is catalyzed by the Cdc7 protein kinase. In contrast, premeiotic DNA replication apparently does not require Cdc...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00360406

    authors: Hollingsworth RE Jr,Sclafani RA

    更新日期:1993-06-01 00:00:00

  • Evolution of DUX gene macrosatellites in placental mammals.

    abstract::Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0380-y

    authors: Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

    更新日期:2012-10-01 00:00:00

  • Epigenetic changes in mammalian gametes throughout their lifetime: the four seasons metaphor.

    abstract::The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-019-00704-w

    authors: Wasserzug-Pash P,Klutstein M

    更新日期:2019-09-01 00:00:00

  • Structural and functional studies on phi 29 DNA polymerase.

    abstract::The Bacillus subtilis phage phi 29 DNA polymerase, involved in protein-primed viral DNA replication, contains several amino acid consensus sequences common to other eukaryotic-type DNA polymerases. Using site-directed mutagenesis, we have studied the functional significance of a C-terminal conserved region, represente...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF02451783

    authors: Blasco MA,Esteban JA,Méndez J,Blanco L,Salas M

    更新日期:1992-01-01 00:00:00

  • Distinct features of nucleolus-associated domains in mouse embryonic stem cells.

    abstract::Heterochromatin in eukaryotic interphase cells frequently localizes to the nucleolar periphery (nucleolus-associated domains (NADs)) and the nuclear lamina (lamina-associated domains (LADs)). Gene expression in somatic cell NADs is generally low, but NADs have not been characterized in mammalian stem cells. Here, we g...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-020-00734-9

    authors: Bizhanova A,Yan A,Yu J,Zhu LJ,Kaufman PD

    更新日期:2020-06-01 00:00:00

  • Amplification of microsatellite repeat motifs is associated with the evolutionary differentiation and heterochromatinization of sex chromosomes in Sauropsida.

    abstract::The sex chromosomes in Sauropsida (reptiles and birds) have evolved independently many times. They show astonishing diversity in morphology ranging from cryptic to highly differentiated sex chromosomes with male (XX/XY) and female heterogamety (ZZ/ZW). Comparing such diverse sex chromosome systems thus provides unpara...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-015-0531-z

    authors: Matsubara K,O'Meally D,Azad B,Georges A,Sarre SD,Graves JA,Matsuda Y,Ezaz T

    更新日期:2016-03-01 00:00:00

  • Recombination correlates with synaptonemal complex length and chromatin loop size in bovids-insights into mammalian meiotic chromosomal organization.

    abstract::Homologous chromosomes exchange genetic information through recombination during meiosis, a process that increases genetic diversity, and is fundamental to sexual reproduction. In an attempt to shed light on the dynamics of mammalian recombination and its implications for genome organization, we have studied the recom...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-016-0624-3

    authors: Ruiz-Herrera A,Vozdova M,Fernández J,Sebestova H,Capilla L,Frohlich J,Vara C,Hernández-Marsal A,Sipek J,Robinson TJ,Rubes J

    更新日期:2017-10-01 00:00:00

  • RNA polymerase B (or II) in heat induced puffs of Drosophila polytene chromosomes.

    abstract::Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329465

    authors: Greenleaf AL,Plagens U,Jamrich M,Bautz EK

    更新日期:1978-01-16 00:00:00

  • 3D genomics imposes evolution of the domain model of eukaryotic genome organization.

    abstract::The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-016-0604-7

    authors: Razin SV,Vassetzky YS

    更新日期:2017-02-01 00:00:00

  • Mechanisms of chromosome banding. VIII. Hoechst 33258-DNA interaction.

    abstract::The interaction of Hoechst 33258 with DNA has been examined to help clarify the mechanisms of banding. 1. In agreement with previous studies Hoechst fluorescence is enhanced to a greater degree in AT-rich compared to GC-rich DNA. 2. Hoechst causes an increase in the DNA Tm which is greater at the higher AT content of ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00332113

    authors: Comings DE

    更新日期:1975-10-14 00:00:00

  • Sex chromosome recombination failure, apoptosis, and fertility in male mice.

    abstract::Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-015-0542-9

    authors: Faisal I,Kauppi L

    更新日期:2016-06-01 00:00:00

  • X-ray induced visible alterations in the giant chromosomes of Phryne cincta (Nematocera, Diptera): relation of radiation sensitivity to pronuclear chromosome structure.

    abstract::In order to induce chromosomal rearrangements, males were exposed to x-rays and then mated to non-irradiated females. The number of each type of structural alteration was determined by examination of the polytene chromosomes of the F1 progeny. -- A comparison of the results with similar studies made on Drosophila reve...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329175

    authors: Israelewski N

    更新日期:1975-12-10 00:00:00

  • Topographic examination of sister chromatid differential staining by Nomarski interference microscopy and scanning electron microscopy.

    abstract::BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00285754

    authors: Takayama S,Utsumi KR,Sasaki Y

    更新日期:1981-01-01 00:00:00

  • Facts and artifacts in studies of gene expression in aneuploids and sex chromosomes.

    abstract::Studies of gene expression in aneuploids have often made the assumption that measurements of RNA abundance from the varied chromosome will establish whether there is a dosage effect or compensation. Typical procedures of RNA isolation and use of equal amounts of RNA for quantitative estimates will not measure the tota...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-014-0478-5

    authors: Birchler JA

    更新日期:2014-10-01 00:00:00

  • Isolation and distribution of a Drosophila protein preferentially associated with inactive regions of the genome.

    abstract::The distribution patterns of chromosomal proteins from Drosophila can be observed by immunofluorescent staining of the polytene chromosomes from larval salivary glands. We have purified a non-histone chromosomal protein of Mr = 69,000 molecular weight which has a high affinity for DNA with little sequence specificity....

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00285889

    authors: Fleischmann G,Filipski R,Elgin SC

    更新日期:1987-01-01 00:00:00

  • Organization of interphase chromatin.

    abstract::The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest ...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-005-0035-3

    authors: Horowitz-Scherer RA,Woodcock CL

    更新日期:2006-02-01 00:00:00

  • Chromosome segregation in crane-fly spermatocytes: cold treatment and cold recovery induce anaphase lag.

    abstract::Anaphase lagging of autosomes was observed in 6.1 +/- 5.4% of the primary spermatocytes in untreated larvae of the crane fly, Nephrotoma suturalis. Lagging was induced by exposure of larvae to 6 degrees C and during recovery at 22 degrees C from exposure to 0.2, 2, and 6 degrees C. The incidence of anaphase lag was ma...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00330776

    authors: Janicke MA,LaFountain JR Jr

    更新日期:1982-01-01 00:00:00

  • Transcription and hybridization of 125I-cRNA from flow sorted chromosomes.

    abstract::Metaphase chromosomes from the Chinese hamster cell line M3-1 were separated by means of a flow sorter. Two chromosome fractions were used for this study: A, which consisted of 95% pure chromosome no. 1, and B, which was 90% pure chromosome no. 2. The DNA of 10(6) chromosomes of each type was purified, and a 125I-cRNA...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328767

    authors: Sawin VL,Rowley JD,Carrano AV

    更新日期:1979-01-31 00:00:00

  • The proteins of polytene chromosomes of Drosophila hydei.

    abstract::It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00319831

    authors: Elgin SC,Boyd JB

    更新日期:1975-01-01 00:00:00

  • Frequency and distribution of chiasmata in Syrian hamster spermatocytes studied by the BrdU antibody technique.

    abstract::The frequency and distribution of chiasmata and the nature of terminal "associations" was re-examined in Syrian hamster spermatocytes using the 5-bromo-2'-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the pen...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF01731702

    authors: Latos-Bielenska A,Vogel W

    更新日期:1990-08-01 00:00:00

  • Versatility at the nuclear pore complex: lessons learned from the nucleoporin Nup153.

    abstract::The vertebrate pore protein Nup153 plays pivotal roles in nuclear pore function. In addition to being important to pore architecture, Nup153 is a key participant in both import and export. The scope of Nup153 function also extends beyond the canonical view of the pore as a trafficking gateway. During the transition in...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-005-0019-3

    authors: Ball JR,Ullman KS

    更新日期:2005-11-01 00:00:00

  • Chromatin structure in the unicellular algae Olisthodiscus luteus, Crypthecodinium cohnii and Peridiniun balticum.

    abstract::Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292229

    authors: Rizzo PJ,Burghardt RC

    更新日期:1980-01-01 00:00:00

  • Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.

    abstract::A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability....

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-014-0489-2

    authors: Tognetti S,Riera A,Speck C

    更新日期:2015-03-01 00:00:00

  • The organization of the evolutionarily conserved GATA/GACA repeats in the mouse genome.

    abstract::Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00386791

    authors: Schäfer R,Ali S,Epplen JT

    更新日期:1986-01-01 00:00:00

  • Changes in DNA topology during spermatogenesis.

    abstract::DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence micros...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288496

    authors: Risley MS,Einheber S,Bumcrot DA

    更新日期:1986-01-01 00:00:00