Abstract:
:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate reconstruction of the DNA synthetic patterns was possible. A direct correlation between the staining intensity of the G bands and the order in which they replicate was found. Dark staining G bands were always the last region of a chromosome to replicate while G negative bands were first. It is concluded that each G band may be a cluster of replicons capable of initiating DNA synthesis simultaneously.
journal_name
Chromosomajournal_title
Chromosomaauthors
Crossen PE,Pathak S,Arrighi FEdoi
10.1007/BF00364018subject
Has Abstractpub_date
1975-11-11 00:00:00pages
339-47issue
4eissn
0009-5915issn
1432-0886journal_volume
52pub_type
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