A high resolution study of the DNA replication patterns of chinese hamster chromosomes using sister chromatid differential staining technique.

Abstract:

:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate reconstruction of the DNA synthetic patterns was possible. A direct correlation between the staining intensity of the G bands and the order in which they replicate was found. Dark staining G bands were always the last region of a chromosome to replicate while G negative bands were first. It is concluded that each G band may be a cluster of replicons capable of initiating DNA synthesis simultaneously.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Crossen PE,Pathak S,Arrighi FE

doi

10.1007/BF00364018

subject

Has Abstract

pub_date

1975-11-11 00:00:00

pages

339-47

issue

4

eissn

0009-5915

issn

1432-0886

journal_volume

52

pub_type

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