Abstract:
:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison with conventional hybridization probes. Southern blot hybridization showed sex-specific patterns with both the (GATA)4 and (GACA)4 oligonucleotide probes, as previously described with conventional probes. The quantitative analysis of two mouse DNA phage libraries and of 25 isolated GATA-positive phage clones revealed intensive interspersion of GATA sequences with GACA, and with other repetitive and single-copy sequences. Ubiquitous interspersion and homogeneous genomic distribution of GATA and GACA sequences were confirmed by hybridization in situ of the oligonucleotide probes to metaphase chromosomes. The lengths of the GATA and GACA stretches were found to vary considerably in the individual phage clones. DNA inserts from 20 phages were assigned to autosomes and sex chromosomes and three genomic fragments were found to be confined to the Y chromosome. The organization of GATA and GACA sequences is discussed in the context of their evolutionary potential and possible conservation mechanisms.
journal_name
Chromosomajournal_title
Chromosomaauthors
Schäfer R,Ali S,Epplen JTdoi
10.1007/BF00386791subject
Has Abstractpub_date
1986-01-01 00:00:00pages
502-10issue
6eissn
0009-5915issn
1432-0886journal_volume
93pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::Thirty P1 clones from the X chromosome (Muller's A element) of Drosophila melanogaster were cross-hybridized in situ to Drosophila subobscura and Drosophila pseudoobscura polytene chromosomes. An additional recombinant phage lambda Dsuby was also used as a marker. Twenty-three (77%) of the P1 clones gave positive hybr...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00347695
更新日期:1995-11-01 00:00:00
abstract::We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female c...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-002-0182-8
更新日期:2002-03-01 00:00:00
abstract::The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Sat...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-005-0343-7
更新日期:2005-07-01 00:00:00
abstract::In wild-type Drosophila hydei (genotype X/Y) four different primary spermatocyte nuclear glycoproteins, classified as non-Y encoded because of their occurrence in X/O genotypes, were demonstrated to possess a few epitopes that depended on formation of the Y chromosomal giant lampbrush loops threads (th; Mr 55,000 prot...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00291050
更新日期:1989-08-01 00:00:00
abstract::The irradiation of metaphase spreads of human cells with ultraviolet (UV) light blocked the chromosome banding induced by Alu I, Mbo I, Dde I, Hinf I, Hae III, and Rsa I restriction endonucleases. At 13 J/m2 there was moderate inhibition of the nuclease action, which was detected as an increase in the stain intensity ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292450
更新日期:1984-01-01 00:00:00
abstract::In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352227
更新日期:1995-10-01 00:00:00
abstract::In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-012-0363-z
更新日期:2012-06-01 00:00:00
abstract::X chromosome inactivation (XCI) is an essential mechanism to compensate gene dosage in mammals. Here, we show that XCI has evolved differently in two species of the genus Tokudaia. The Amami spiny rat, Tokudaia osimensis, has a single X chromosome in males and females (XO/XO). By contrast, the Okinawa spiny rat, Tokud...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-017-0639-4
更新日期:2017-12-01 00:00:00
abstract::In contrast to yeast, plant interphase nuclei often display incomplete alignment (cohesion) along sister chromatid arms. Sister chromatid cohesion mediated by the multi-subunit cohesin complex is essential for correct chromosome segregation during nuclear divisions and for DNA recombination repair. The cohesin complex...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-009-0220-x
更新日期:2009-10-01 00:00:00
abstract::cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294968
更新日期:1982-01-01 00:00:00
abstract::We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293158
更新日期:1985-01-01 00:00:00
abstract::Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-006-0087-z
更新日期:2007-04-01 00:00:00
abstract::Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-012-0380-y
更新日期:2012-10-01 00:00:00
abstract::The localization of DNA in the condensed interphase chromosomes of Euglena was determined by immunoelectron microscopy. Deposits of gold particles that coincided with the localization of DNA followed threads that corresponded to the chromatin fibers. The threads were 55-80nm in diameter and were assumed to be supersol...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00337227
更新日期:1996-01-01 00:00:00
abstract::Using electron spectroscopic imaging, a new type of small granular structural constituent has been observed in the extrachromosomal zone of the polytene nucleus of the salivary gland cells of Chironomus thummi and Chironomus tentans. These granules appear isolated or in small clumps and are often seen to be connected ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00650895
更新日期:1993-12-01 00:00:00
abstract::Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0512-2
更新日期:2015-12-01 00:00:00
abstract::Studies of gene expression in aneuploids have often made the assumption that measurements of RNA abundance from the varied chromosome will establish whether there is a dosage effect or compensation. Typical procedures of RNA isolation and use of equal amounts of RNA for quantitative estimates will not measure the tota...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-014-0478-5
更新日期:2014-10-01 00:00:00
abstract::Cyclins, as regulatory partners of cyclin-dependent kinases (CDKs), control the switch-like cell cycle transitions that orchestrate orderly duplication and segregation of genomes. Compared to mitosis, relatively little is known about how cyclin-CDK complexes control meiosis, the specialized cell division that generate...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00725-5
更新日期:2019-09-01 00:00:00
abstract::Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-003-0233-9
更新日期:2003-04-01 00:00:00
abstract::Replication times for all important chromosome bands, of both types R and Q (277 structures) are analysed. The R-bands form a group of structures whose DNA replicates during the early S-phase, while the DNA situated in the Q-bands replicates during the late S-phase. There may not exist overlapping between replication ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293440
更新日期:1976-10-12 00:00:00
abstract::We have sequenced and analyzed 8.3 kb of sequence adjacent and distal to the human ribosomal DNA (rDNA); this distal sequence connects to the rDNA cluster just 4 kb upstream of the first promoter and is shared among the acrocentric chromosomes and, at least in part, it is also present in other primates. The sequence d...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF02510479
更新日期:1997-06-01 00:00:00
abstract::In order to induce chromosomal rearrangements, males were exposed to x-rays and then mated to non-irradiated females. The number of each type of structural alteration was determined by examination of the polytene chromosomes of the F1 progeny. -- A comparison of the results with similar studies made on Drosophila reve...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329175
更新日期:1975-12-10 00:00:00
abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293338
更新日期:1989-06-01 00:00:00
abstract::The divergence of lineages leading to extant squamate reptiles (lizards, snakes, and amphisbaenians) and birds occurred about 275 million years ago. Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. Hence, a number of chromosome rearrangements might be ex...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0322-0
更新日期:2011-10-01 00:00:00
abstract::While many studies have provided significant insight into homolog pairing during meiosis, information on non-homologous pairing is much less abundant. In the present study, fluorescence in situ hybridization (FISH) was used to investigate non-homologous pairing in haploid rice during meiosis. At pachytene, non-homolog...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-010-0288-3
更新日期:2011-02-01 00:00:00
abstract::The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00302363
更新日期:1988-01-01 00:00:00
abstract::A karyotype based on banding pattern and chromosome length is presented for the white-handed gibbon, Hylobates lar. Little homology with the banding patterns of the chromosomes of the other Hominoidea can be seen, confirming the early evolutionary separation of Hylobatidae and the other apes. Hybridization in situ wit...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285805
更新日期:1975-01-01 00:00:00
abstract::Insulator proteins orchestrate the three-dimensional organization of the genome. Insulators function by facilitating communications between regulatory sequences and gene promoters, allowing accurate gene transcription regulation during embryo development and cell differentiation. However, the role of insulator protein...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-020-00743-8
更新日期:2020-12-01 00:00:00
abstract::The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degene...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-006-0086-0
更新日期:2007-04-01 00:00:00
abstract::Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0403-3
更新日期:2013-06-01 00:00:00