Abstract:
:Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inactivation of Mod(mdg4)-67.2 leads to a direct repression of the yellow gene promoter by the gypsy insulator. Here, we have shown that such repression is regulated by the level of the EAST protein, which is an essential component of the interchromatin compartment. Deletion of the EAST C-terminal domain suppresses Su(Hw)-mediated repression. Partial inactivation of EAST by mutations in the east gene suppresses the enhancer-blocking activity of the gypsy insulator. The binding of insulator proteins to chromatin is highly sensitive to the level of EAST expression. These results suggest that EAST, one of the main components of the interchromatin compartment, can regulate the activity of chromatin insulators.
journal_name
Chromosomajournal_title
Chromosomaauthors
Melnikova L,Shapovalov I,Kostyuchenko M,Georgiev P,Golovnin Adoi
10.1007/s00412-016-0596-3subject
Has Abstractpub_date
2017-03-01 00:00:00pages
299-311issue
2eissn
0009-5915issn
1432-0886pii
10.1007/s00412-016-0596-3journal_volume
126pub_type
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