Selective digestion of mouse metaphase chromosomes.

abstract：:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...

journal_title：Chromosoma

authors： Melnikova L,Kostyuchenko M,Molodina V,Parshikov A,Georgiev P,Golovnin A

更新日期：2018-03-01 00:00:00

abstract：:By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...

journal_title：Chromosoma

authors： Livolant F

更新日期：1978-08-21 00:00:00

abstract：:In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...

journal_title：Chromosoma

authors： Qiao H,Offenberg HH,Anderson LK

更新日期：2012-06-01 00:00:00

abstract：:PICH (Plk1-interacting checkpoint helicase) was recently identified as an essential component of the spindle assembly checkpoint and shown to localize to kinetochores, inner centromeres, and thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in si...

journal_title：Chromosoma

authors： Wang LH,Schwarzbraun T,Speicher MR,Nigg EA

更新日期：2008-04-01 00:00:00

abstract：:The single-stranded DNA binding protein RP-A is required in SV40 DNA in vitro replication. The RP-A purified from calf thymus contains 4 polypeptides with molecular weights 70kDa, 53kDa, 32kDa, and 14kDa. The p70 subunit and its proteolysed form p53 are recognized by the monoclonal antibody 70C (Kenny et al. (1990)) a...

journal_title：Chromosoma

authors： Nasheuer HP,von Winkler D,Schneider C,Dornreiter I,Gilbert I,Fanning E

更新日期：1992-01-01 00:00:00

abstract：:In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this techniqu...

journal_title：Chromosoma

authors： Liu JY,She CW,Hu ZL,Xiong ZY,Liu LH,Song YC

更新日期：2004-08-01 00:00:00

abstract：:Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...

journal_title：Chromosoma

authors： Han Y,Wang G,Liu Z,Liu J,Yue W,Song R,Zhang X,Jin W

更新日期：2010-02-01 00:00:00

abstract：:Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...

journal_title：Chromosoma

authors： Mousson F,Ochsenbein F,Mann C

更新日期：2007-04-01 00:00:00

abstract：:The members of the serine-arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle between the nucleus and the cytopl...

journal_title：Chromosoma

authors： Singh OP,Visa N,Wieslander L,Daneholt B

更新日期：2006-12-01 00:00:00

abstract：:We report on the construction of the first comparative Zoo-FISH map of a marine mammal. Zoo-FISH with DNA probes from a human chromosome-specific library to metaphase spreads of the harbor seal (Phoca vitulina) disclosed 31 conserved syntenic segments covering the complete autosomal complement and the X chromosome. Co...

journal_title：Chromosoma

authors： Frönicke L,Müller-Navia J,Romanakis K,Scherthan H

更新日期：1997-07-01 00:00:00

abstract：:The molecular structure of chromatin during dogfish spermiogenesis was examined by electron microscopy after the dispersion of nuclei at low ionic strength. In early and late stages of differentiation (round and elongating spermatids), chromatin is globular, although basic nuclear proteins are different from those pre...

journal_title：Chromosoma

authors： Gusse M,Chevaillier P

更新日期：1980-01-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00

abstract：:The nature of replication origins in eukaryotic chromosomes has been examined in some detail only in yeast, Drosophila, and mammalian cells. We have used highly synchronous cultures of plasmodia of the myxomycete Physarum and two-dimensional agarose gel electrophoresis to examine replication of two developmentally con...

journal_title：Chromosoma

authors： Diller JD,Sauer HW

更新日期：1993-09-01 00:00:00

abstract：:The hsp70, hsp83, hsromega, and the small heat shock protein genes were mapped on the polytene chromosomes of six species, representative of the geographical distribution of the Drosophila montium subgroup of the melanogaster species group. In addition, based on hybridization conditions, the putative locus of the hsp6...

journal_title：Chromosoma

authors： Drosopoulou E,Konstantopoulou I,Scouras ZG

更新日期：1996-08-01 00:00:00

abstract：:We describe a mammalian artificial mini-chromosome lacking human alphoid DNA and mouse minor and major satellite DNA repeats. This mini-chromosome, initially recovered in a mouse embryonic stem (ES) cell line (CGR8), is 2.6 Mb in size and consists of sequences derived from the human Y chromosome and mouse chromosomes ...

journal_title：Chromosoma

authors： Shen MH,Ross A,Yang J,de las Heras JI,Cooke H

更新日期：2001-11-01 00:00:00

abstract：:We describe a method for preparing nuclear spreads from cells of live, unfixed zebrafish embryos at the late-gastrula (approximately 8000 cell) stage of development. The method consists of a sequence of four steps: (1) a slow, gentle lysis, in low to moderate salt concentration, of cells and then nuclei, to release DN...

journal_title：Chromosoma

authors： Chan DW,Yager TD

更新日期：1998-03-01 00:00:00

abstract：:The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres vari...

journal_title：Chromosoma

authors： Lermontova I,Rutten T,Schubert I

更新日期：2011-12-01 00:00:00

abstract：:In Drosophila gibberosa, differences between midgut and salivary gland chromosomes fall into two categories: tissue-specific band modulations which persist throughout the 90 h developmental period that we studied and tissue-specific puffs. Puffs that are common to both tissues tend to appear earlier in the midgut. Som...

journal_title：Chromosoma

authors： Roberts PA

更新日期：1988-11-01 00:00:00

abstract：:BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment...

journal_title：Chromosoma

authors： Takayama S,Utsumi KR,Sasaki Y

更新日期：1981-01-01 00:00:00

abstract：:Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...

journal_title：Chromosoma

authors： Taruscio D,Manuelidis L

更新日期：1991-12-01 00:00:00

abstract：:This paper describes the effects of 5-azacytidine on the condensation state of rye (Secale cereale L.) chromatin introduced into the wheat genome (Triticum aestivum L. cv. Beaver). The wheat cultivar Beaver carries a translocation between the short arm of rye chromosome 1R (1RS) and the long arm of wheat chromosome 1B...

journal_title：Chromosoma

authors： Glyn MC,Egertová M,Gazdova B,Kovarik A,Bezdek M,Leitch AR

更新日期：1997-12-01 00:00:00

abstract：:We have sequenced and analyzed 8.3 kb of sequence adjacent and distal to the human ribosomal DNA (rDNA); this distal sequence connects to the rDNA cluster just 4 kb upstream of the first promoter and is shared among the acrocentric chromosomes and, at least in part, it is also present in other primates. The sequence d...

journal_title：Chromosoma

authors： Gonzalez IL,Sylvester JE

更新日期：1997-06-01 00:00:00

abstract：:Osteosarcoma (OS) is characterized by chromosomal instability and high copy number gene amplification. The breakage-fusion-bridge (BFB) cycle is a well-established mechanism of genome instability in tumors and in vitro models used to study the origins of complex chromosomal rearrangements and cancer genome amplificati...

journal_title：Chromosoma

authors： Selvarajah S,Yoshimoto M,Park PC,Maire G,Paderova J,Bayani J,Lim G,Al-Romaih K,Squire JA,Zielenska M

更新日期：2006-12-01 00:00:00

abstract：:The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...

journal_title：Chromosoma

authors： Zacharias H

更新日期：1984-01-01 00:00:00

abstract：:Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...

journal_title：Chromosoma

authors： Mancino G,Ragghianti M,Bucci-Innocenti S

更新日期：1979-08-10 00:00:00

abstract：:To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...

journal_title：Chromosoma

authors： Maeshima K,Eltsov M,Laemmli UK

更新日期：2005-11-01 00:00:00

abstract：:The dioecious plant Silene latifolia has large, heteromorphic X and Y sex chromosomes that are thought to be derived from rearrangements of autosomes. To reveal the origin of the sex chromosomes in S. latifolia, we isolated and characterized telomere-homologous sequences from intra-chromosomal regions (interstitial te...

journal_title：Chromosoma

authors： Uchida W,Matsunaga S,Sugiyama R,Shibata F,Kazama Y,Miyazawa Y,Hizume M,Kawano S

更新日期：2002-12-01 00:00:00

abstract：:Thirty P1 clones from the X chromosome (Muller's A element) of Drosophila melanogaster were cross-hybridized in situ to Drosophila subobscura and Drosophila pseudoobscura polytene chromosomes. An additional recombinant phage lambda Dsuby was also used as a marker. Twenty-three (77%) of the P1 clones gave positive hybr...

journal_title：Chromosoma

authors： Segarra C,Lozovskaya ER,Ribó G,Aguadé M,Hartl DL

更新日期：1995-11-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain contro...

journal_title：Chromosoma

authors： Paschal CR,Maciejowski J,Jallepalli PV

更新日期：2012-12-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...

journal_title：Chromosoma

authors： Capozzi O,Archidiacono N,Lorusso N,Stanyon R,Rocchi M

更新日期：2016-09-01 00:00:00