Abstract:
:The single-stranded DNA binding protein RP-A is required in SV40 DNA in vitro replication. The RP-A purified from calf thymus contains 4 polypeptides with molecular weights 70kDa, 53kDa, 32kDa, and 14kDa. The p70 subunit and its proteolysed form p53 are recognized by the monoclonal antibody 70C (Kenny et al. (1990)) and bind to ssDNA. The p70 and p32 subunits of bovine RP-A are phosphorylated by CDC2-cyclin B kinase. Bovine RP-A supports the origin dependent unwinding of SV40 DNA by T antigen. Furthermore, bovine RP-A can efficiently substitute for human RP-A in SV40 DNA replication in vitro. A modified blotting technique revealed that RP-A interacts specifically and directly with the p48 subunit of DNA polymerase alpha-primase complex.
journal_name
Chromosomajournal_title
Chromosomaauthors
Nasheuer HP,von Winkler D,Schneider C,Dornreiter I,Gilbert I,Fanning Edoi
10.1007/BF02451786subject
Has Abstractpub_date
1992-01-01 00:00:00pages
S52-9issue
1 Suppleissn
0009-5915issn
1432-0886journal_volume
102pub_type
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