Abstract:
:A method is described for excising by a glass knife single bands of isolated polytene chromosomes of the salivary glands of Chironomus tentans larvae. DNA strands were isolated from cut-out bands and their contour lengths were determined on electron micrographs. The mean contour length of DNA strands isolated from the double band I-8A was about twice that of the single band I-11B, namely 63 versus 34 micrometers. The described method may be applicable for molecular studies on single bands (e.g., by DNA cloning).
journal_name
Chromosomajournal_title
Chromosomaauthors
Frey M,Koller T,Lezzi Mdoi
10.1007/BF00292850subject
Has Abstractpub_date
1982-01-01 00:00:00pages
493-503issue
4eissn
0009-5915issn
1432-0886journal_volume
84pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293338
更新日期:1989-06-01 00:00:00
abstract::BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285754
更新日期:1981-01-01 00:00:00
abstract::The large heterochromatic chromosome of Drosophila nasutoides reveals distinctive C- and Q-bands. The regions which are negative in C-banding appear positive in Q-banding. The isochromosomic nature of this chromosome and the locality of the satellite DNAs in this chromosome are discussed with respect to these banding ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292680
更新日期:1977-04-27 00:00:00
abstract::When A-banded human chromosomes mounted in water are transferred to an organic mounting medium, the chromosomes show uniform bright quinacrine fluorescence. This change is reversible. It is inferred that quinacrine is bound uniformly along the chromosomes, and that Q-banding is a consequence of a non-uniform distribut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294941
更新日期:1977-12-06 00:00:00
abstract::Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00692-x
更新日期:2019-09-01 00:00:00
abstract::Previous studies of the Robertsonian polymorphism in the Atlantic dog-whelk, Nucella lapillus (2n = 26-36), have been limited by the inability to identify unequivocally individual chromosomes in the karyotype. This species, as with many other marine invertebrates, has proven largely refractory to the standard (mammali...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352269
更新日期:1996-03-01 00:00:00
abstract::While many studies have provided significant insight into homolog pairing during meiosis, information on non-homologous pairing is much less abundant. In the present study, fluorescence in situ hybridization (FISH) was used to investigate non-homologous pairing in haploid rice during meiosis. At pachytene, non-homolog...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-010-0288-3
更新日期:2011-02-01 00:00:00
abstract::Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327329
更新日期:1983-01-01 00:00:00
abstract::The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X c...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00356024
更新日期:1993-01-01 00:00:00
abstract::The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327377
更新日期:1978-10-20 00:00:00
abstract::A clone of highly repetitive DNA, designated C5, was isolated from DNA of female Chinese muntjac cells. The nucleotide sequence of this clone is 80%-85% homologous to that of the satellite IA clone and other highly repetitive DNA clones previously obtained from the Indian muntjac. Using C5 as a probe for in situ hybri...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00360682
更新日期:1991-10-01 00:00:00
abstract::The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest ...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-005-0035-3
更新日期:2006-02-01 00:00:00
abstract::At least two types of Robertsonian exchange are now known in the acrocentric chromosomes of man. Both types involve breakage in the arms adjacent to the centromere. Evidence is presented for a third type of exchange, one involving breakage within the centromere itself, in the grasshopper Percassa rugifrons. In this sp...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00326262
更新日期:1975-09-26 00:00:00
abstract::Dioecious Silene latifolia evolved heteromorphic sex chromosomes within the last ten million years, making it a species of choice for studies of the early stages of sex chromosome evolution in plants. About a dozen genes have been isolated from its sex chromosomes and basic genetic and deletion maps exist for the X an...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0311-3
更新日期:2011-06-01 00:00:00
abstract::Antibodies against human Rad51 protein were used to examine the distribution of Rad51 on meiotic chromatin in mouse spermatocytes and oocytes as well as chicken oocytes during sequential stages of meiosis. We observed the following dynamic changes in distribution of Rad51 during meiosis: (1) in early leptotene nuclei ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352222
更新日期:1995-10-01 00:00:00
abstract::DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Triturus cristatus (23 pg) and Necturus maculosus (52 pg) of the ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327706
更新日期:1976-12-06 00:00:00
abstract::From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00344161
更新日期:1991-05-01 00:00:00
abstract::A higher order organization of the centromeres in the form of clustering of these DNA loci has been observed in many organisms. While centromere clustering is biologically significant to achieve faithful chromosome segregation, the underlying molecular mechanism is yet to be fully understood. In budding yeast, a kinet...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00694-9
更新日期:2019-06-01 00:00:00
abstract::In Drosophila gibberosa, differences between midgut and salivary gland chromosomes fall into two categories: tissue-specific band modulations which persist throughout the 90 h developmental period that we studied and tissue-specific puffs. Puffs that are common to both tissues tend to appear earlier in the midgut. Som...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292969
更新日期:1988-11-01 00:00:00
abstract::Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-003-0233-9
更新日期:2003-04-01 00:00:00
abstract::Among his many contributions to the field of chromosome structure and dynamics, J. Herbert Taylor showed that eukaryotic cells have many more potential replication origins than they use, which they can recruit when replication forks are slowed to complete S-phase in a timely fashion. Thirty years later, his findings r...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-007-0105-9
更新日期:2007-08-01 00:00:00
abstract::Metaphase chromosomes from the Chinese hamster cell line M3-1 were separated by means of a flow sorter. Two chromosome fractions were used for this study: A, which consisted of 95% pure chromosome no. 1, and B, which was 90% pure chromosome no. 2. The DNA of 10(6) chromosomes of each type was purified, and a 125I-cRNA...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328767
更新日期:1979-01-31 00:00:00
abstract::The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293176
更新日期:1978-06-28 00:00:00
abstract::Fluorescent in situ hybridization with chromosome specific probes was used in conjunction with laser scanning confocal microscopy to assess the three-dimensional distribution of chromosomes in human T-lymphocyte nuclei. Cells in the G1-phase of the cell cycle exhibit a distinctly non-random chromosome organization:cen...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00660315
更新日期:1992-08-01 00:00:00
abstract::The dioecious plant Silene latifolia has large, heteromorphic X and Y sex chromosomes that are thought to be derived from rearrangements of autosomes. To reveal the origin of the sex chromosomes in S. latifolia, we isolated and characterized telomere-homologous sequences from intra-chromosomal regions (interstitial te...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-002-0213-5
更新日期:2002-12-01 00:00:00
abstract::Feulgen cytophotometric measurements of neuroblasts in the first and third instar larvae of Drosophila melanogaster reveal the same DNA content for metaphases with chromosomes of different size. The total absorbance of all measured metaphases gives the four-fold value of that of the spermatids. Accordingly there seem ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327078
更新日期:1975-01-01 00:00:00
abstract::The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293452
更新日期:1976-02-23 00:00:00
abstract::Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294845
更新日期:1979-06-21 00:00:00
abstract::The divergence of lineages leading to extant squamate reptiles (lizards, snakes, and amphisbaenians) and birds occurred about 275 million years ago. Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. Hence, a number of chromosome rearrangements might be ex...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0322-0
更新日期:2011-10-01 00:00:00
abstract::The chromosomes of lake trout (Salvelinus namaycush) contain a considerable amount of heterochromatin located at the centromeres and/or telomeres of several chromosomes, including a sex-specific block located distally on the X chromosome. In order to investigate further the repetitive DNAs of lake trout, genomic DNA f...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352255
更新日期:1995-12-01 00:00:00