Abstract:
:From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the Adh gene of D. melanogaster, which has been described earlier to have ARS function in yeast, were microinjected into developing Drosophila eggs and analysed after reisolation from first instar larvae. As an assay for DNA replication, we determined the sensitivity of recovered plasmid DNA to restriction enzymes that discriminate between adenine methylation and non-methylation. Within the limits of detection our results show that none of the plasmids replicated two or more rounds. However, a fraction of all injected plasmid DNAs, including vector DNA, seems to replicate once. The same result was obtained for a DNA sequence from mouse that had been reported to have replication origin function in mouse tissue culture cells. We excluded the possibility that methylation of the plasmids is the reason for their inability to replicate. These results demonstrate that homologous and heterologous DNA sequences that drive replication of plasmids in cells of other species are not sufficient to fulfil this function in Drosophila embryos.
journal_name
Chromosomajournal_title
Chromosomaauthors
Roth GEdoi
10.1007/BF00344161subject
Has Abstractpub_date
1991-05-01 00:00:00pages
267-77issue
4eissn
0009-5915issn
1432-0886journal_volume
100pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::With simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifie...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00362284
更新日期:1994-10-01 00:00:00
abstract::Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294783
更新日期:1987-01-01 00:00:00
abstract::An inverted duplication of chromosome 15 [inv dup(15)] is the most common supernumerary marker chromosome, comprising approximately 50% of all chromosomes in this class. Structurally, the inv dup(15) is a mirror image with the central axis defining a distal break within either the heterochromatic alpha-satellite array...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120000103
更新日期:2000-11-01 00:00:00
abstract::Studies of gene expression in aneuploids have often made the assumption that measurements of RNA abundance from the varied chromosome will establish whether there is a dosage effect or compensation. Typical procedures of RNA isolation and use of equal amounts of RNA for quantitative estimates will not measure the tota...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-014-0478-5
更新日期:2014-10-01 00:00:00
abstract::Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromoso...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00326317
更新日期:1975-08-11 00:00:00
abstract::The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293114
更新日期:1976-06-30 00:00:00
abstract::The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00337600
更新日期:1990-12-01 00:00:00
abstract::Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327453
更新日期:1977-09-27 00:00:00
abstract::Of 204 mutations located in the 8-12 band Df(2L)130 region, 37B9-C1,2;37D1-2, 199 have been assigned to twelve lethal genes and one visible gene (hook). The 13 genes are not evenly distributed. Twelve, (possibly all thirteen) are in the seven band region 37B10-C4 giving a gene-to-band ratio of almost two. Only one gen...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286015
更新日期:1981-01-01 00:00:00
abstract::A karyotype based on banding pattern and chromosome length is presented for the white-handed gibbon, Hylobates lar. Little homology with the banding patterns of the chromosomes of the other Hominoidea can be seen, confirming the early evolutionary separation of Hylobatidae and the other apes. Hybridization in situ wit...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285805
更新日期:1975-01-01 00:00:00
abstract::Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0403-3
更新日期:2013-06-01 00:00:00
abstract::Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-003-0233-9
更新日期:2003-04-01 00:00:00
abstract::The influence of the sex-reversal factor (Sxr) on X and Y chromosome pairing was examined by comparing males with "novel" and "standard" Sxr genotypes. The novel Sxr males were exceptional in carrying Sxr on their X rather than their Y chromosome, or homozygously on both their X and Y chromosomes, or on a Y chromosome...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292766
更新日期:1989-03-01 00:00:00
abstract::Macronuclear DNA was isolated from Paramecium primaurelia, stock 168. Although the macronucleus is polyploid to the extent of 840C, in other respect the DNA appears to be simply organized, having neither satellite sequences nor substantial amounts of intermediately repetitive sequence. The sequence complexity of macro...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328389
更新日期:1980-01-01 00:00:00
abstract::The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352202
更新日期:1991-09-01 00:00:00
abstract::Insulator proteins orchestrate the three-dimensional organization of the genome. Insulators function by facilitating communications between regulatory sequences and gene promoters, allowing accurate gene transcription regulation during embryo development and cell differentiation. However, the role of insulator protein...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-020-00743-8
更新日期:2020-12-01 00:00:00
abstract::Metaphase chromosomes prepared from colcemid-treated mouse L929 cells by non-ionic detergent lysis exhibit distinct heterochromatic centromere regions and associated kinetochores when viewed by whole mount electron microscopy. Deoxyribonuclease I treatment of these chromosomes results in the preferential digestion of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330554
更新日期:1978-04-25 00:00:00
abstract::Aurora-A is a conserved mitotic kinase overexpressed in many types of cancer. Growing evidence shows that Aurora-A plays a crucial role in DNA damage response (DDR) although this aspect has been less characterized. We isolated a new aur-A mutation, named aur-A949, in Drosophila, and we showed that it causes chromosome...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-018-00687-0
更新日期:2019-03-01 00:00:00
abstract::Dioecious Silene latifolia evolved heteromorphic sex chromosomes within the last ten million years, making it a species of choice for studies of the early stages of sex chromosome evolution in plants. About a dozen genes have been isolated from its sex chromosomes and basic genetic and deletion maps exist for the X an...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0311-3
更新日期:2011-06-01 00:00:00
abstract::Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286046
更新日期:1977-07-08 00:00:00
abstract::P elements, a family of DNA transposons, are known as aggressive intruders into the hitherto uninfected gene pool of Drosophila melanogaster. Invading through horizontal transmission from an external source they managed to spread rapidly through natural populations within a few decades. Owing to their propensity for r...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s004120100144
更新日期:2001-07-01 00:00:00
abstract::An unstable minichromosome comprising part of the short arm of chromosome 10 of maize was recovered from an oil yellow variegated plant as a consequence of gamma irradiation of pollen. The cytological and gene dosage observations are consistent with the minichromosome being a partial isochromosome, which lags at mitot...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352297
更新日期:1996-06-01 00:00:00
abstract::We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is charac...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01731720
更新日期:1990-09-01 00:00:00
abstract::DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence micros...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288496
更新日期:1986-01-01 00:00:00
abstract::Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0325-x
更新日期:2011-08-01 00:00:00
abstract::The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres vari...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0338-5
更新日期:2011-12-01 00:00:00
abstract::Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352322
更新日期:1994-04-01 00:00:00
abstract::In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this techniqu...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-004-0289-1
更新日期:2004-08-01 00:00:00
abstract::The localization of DNA in the condensed interphase chromosomes of Euglena was determined by immunoelectron microscopy. Deposits of gold particles that coincided with the localization of DNA followed threads that corresponded to the chromatin fibers. The threads were 55-80nm in diameter and were assumed to be supersol...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00337227
更新日期:1996-01-01 00:00:00
abstract::The cotyledon parenchyma cells of common bean (Phaseolus vulgaris L.) produce large quantities of storage proteins during embryo maturation; throughout this period, these cells also accumulate nuclear DNA (nDNA). To investigate the basis of this nDNA accumulation, we have measured storage protein mRNA pools, nDNA mass...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01731133
更新日期:1990-07-01 00:00:00