Abstract:
:Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of semi-conservative meiotic DNA synthesis, essential to the biochemical analysis of pachytene DNA metabolism, has been achieved by in vivo administration of 1 M hydroxyurea.
journal_name
Chromosomajournal_title
Chromosomaauthors
Chandley AC,Hotta Y,Stern Hdoi
10.1007/BF00286046subject
Has Abstractpub_date
1977-07-08 00:00:00pages
243-53issue
3eissn
0009-5915issn
1432-0886journal_volume
62pub_type
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