Abstract:
:DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Triturus cristatus (23 pg) and Necturus maculosus (52 pg) of the Urodela subclass. Within each subclass the two species studied were found to have about the same absolute amount of unique DNA. The differences of total nuclear DNA can be accounted for by quantitative variations of the repetitive sequence classes, at least in part due to changes in the number of copies of the various sequences. On the contrary the great difference in nuclear DNA between the two subclasses, Anura and Urodela, involves all sequence classes in parallel; the slowly reassociating fraction appears to be unique in spite of a tenfold difference in absolute amount. The dependence of reassociation kinetics on DNA fragment length for the four species indicates for all of them an interspersed organization of the various sequence classes.
journal_name
Chromosomajournal_title
Chromosomaauthors
Baldari CT,Amaldi Fdoi
10.1007/BF00327706subject
Has Abstractpub_date
1976-12-06 00:00:00pages
13-22issue
1eissn
0009-5915issn
1432-0886journal_volume
59pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-016-0596-3
更新日期:2017-03-01 00:00:00
abstract::We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female c...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-002-0182-8
更新日期:2002-03-01 00:00:00
abstract::In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-007-0123-7
更新日期:2008-02-01 00:00:00
abstract::The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01735325
更新日期:1990-04-01 00:00:00
abstract::The appearance of RNP droplets associated with specific regions of polytene chromosomes from Chironomus larvae has been studied, systematically by light microscopy using glutarladehyde fixation and lactic acid squashing of the salivary gland. During the fourth larval instar the highest frequency of nuclei which contai...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00344485
更新日期:1979-09-01 00:00:00
abstract::Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285773
更新日期:1981-01-01 00:00:00
abstract::The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Sat...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-005-0343-7
更新日期:2005-07-01 00:00:00
abstract::We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293158
更新日期:1985-01-01 00:00:00
abstract::Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences we...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294081
更新日期:1975-12-29 00:00:00
abstract::The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-016-0604-7
更新日期:2017-02-01 00:00:00
abstract::The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that ...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-014-0463-z
更新日期:2014-08-01 00:00:00
abstract::The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288332
更新日期:1976-03-31 00:00:00
abstract::Almost half of the human genome consists of repetitive DNA. Understanding what role these elements have in setting up chromatin states that underlie gene and chromosome function in complex genomes is paramount. The function of some types of repetitive DNA is obvious by virtue of their location, such as the alphoid arr...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-009-0233-5
更新日期:2009-12-01 00:00:00
abstract::Chromosome behaviour during meiosis in male Syrian hamsters heterozygous for one of three translocations was analysed as part of a study of the transmission of these structural changes. Synapsis was studied using preparations of synaptonemal complexes, and chiasmate associations and the results of anaphase I segregati...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327317
更新日期:1989-10-01 00:00:00
abstract::The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120000077
更新日期:2000-07-01 00:00:00
abstract::The Smc5/6 complex, along with cohesin and condensin, is a member of the structural maintenance of chromosome (SMC) family, large ring-like protein complexes that are essential for chromatin structure and function. Thanks to numerous studies of the mitotic cell cycle, Smc5/6 has been implicated to have roles in homolo...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-015-0518-9
更新日期:2016-03-01 00:00:00
abstract::Metaphase chromosomes from the Chinese hamster cell line M3-1 were separated by means of a flow sorter. Two chromosome fractions were used for this study: A, which consisted of 95% pure chromosome no. 1, and B, which was 90% pure chromosome no. 2. The DNA of 10(6) chromosomes of each type was purified, and a 125I-cRNA...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328767
更新日期:1979-01-31 00:00:00
abstract::Genomic sequencing reveals similar but limited numbers of protein-coding genes in different genomes, which begs the question of how organismal diversities are generated. Alternative pre-mRNA splicing, a widespread phenomenon in higher eukaryotic genomes, is thought to provide a mechanism to increase the complexity of ...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-013-0407-z
更新日期:2013-06-01 00:00:00
abstract::The sex chromosomes in Sauropsida (reptiles and birds) have evolved independently many times. They show astonishing diversity in morphology ranging from cryptic to highly differentiated sex chromosomes with male (XX/XY) and female heterogamety (ZZ/ZW). Comparing such diverse sex chromosome systems thus provides unpara...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0531-z
更新日期:2016-03-01 00:00:00
abstract::The organization of DNA in the mitotic metaphase and polytene chromosomes of the fungus gnat, Sciara coprophila, has been studied using base-specific DNA ligands, including anti-nucleoside antibodies. The DNA of metaphase and polytene chromosomes reacts with AT-specific probes (quinacrine, DAPI, Hoechst 33258 and anti...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327321
更新日期:1980-01-01 00:00:00
abstract::Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded state...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0445-6
更新日期:2014-06-01 00:00:00
abstract::The location of DNA fragments representing mobile dispersed genes (MDG) in salivary gland and midgut polytene chromosomes was compared by means of in situ hybridization. In the Drosophila stock under study the average number of hybridization sites in the polytene chromosomes of one nucleus was 20 for MDG-1 and 10 for ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285767
更新日期:1981-01-01 00:00:00
abstract::The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352253
更新日期:1994-07-01 00:00:00
abstract::Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0512-2
更新日期:2015-12-01 00:00:00
abstract::By means of a cytological technique involving 5-bromodeoxyuridine, acridine orange, and fluorescence microscopy, the asynchronously replicating, hence genetically inactivated, X chromosome was identified in 6- to 8-day embryos from female mice heterozygous for Searle's translocation T(X;16)16H (abbreviated as T16H) ma...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00368155
更新日期:1980-01-01 00:00:00
abstract::The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-017-0645-6
更新日期:2018-03-01 00:00:00
abstract::Rice (Oryza sativa L.) centromeres are composed of 155-bp satellite repeats (CentO), centromere-specific retrotransposon (CRR), and a variety of other repeats. Previous studies have shown that CentO and CRR elements are both parts of the functional centromere/kinetochore complex. In this study, a naturally occurring k...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0413-1
更新日期:2013-08-01 00:00:00
abstract::Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050304
更新日期:1998-09-01 00:00:00
abstract::The number of RNA molecules per cell (transcriptome size) is highly variable, differing among and within cell types depending on cell size, stage of the cell cycle, ploidy level, age, disease state, and growth condition. Such variation has been observed at the level of total RNA, ribosomal RNA, messenger RNA (mRNA), a...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-014-0496-3
更新日期:2015-03-01 00:00:00
abstract::In Drosophila as in many organisms beta tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the beta 1 and beta 2 tubulins of Drosophila melanogaster and the beta 2 tubulin of D. hydei, and found these insect beta tubulins to be highly conserved and like beta tubulins...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00333989
更新日期:1987-01-01 00:00:00