Assembly and nuclear export of pre-ribosomal particles in budding yeast.

abstract：:Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...

journal_title：Chromosoma

authors： Eichenlaub-Ritter U,Ruthmann A

更新日期：1982-01-01 00:00:00

abstract：:Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...

journal_title：Chromosoma

authors： Dowsett AP

更新日期：1983-01-01 00:00:00

abstract：:To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...

journal_title：Chromosoma

authors： Maeshima K,Eltsov M,Laemmli UK

更新日期：2005-11-01 00:00:00

abstract：:Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...

journal_title：Chromosoma

authors： Yamamoto Y,Gerbi SA

更新日期：2018-12-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00

abstract：:The sex chromosomes in Sauropsida (reptiles and birds) have evolved independently many times. They show astonishing diversity in morphology ranging from cryptic to highly differentiated sex chromosomes with male (XX/XY) and female heterogamety (ZZ/ZW). Comparing such diverse sex chromosome systems thus provides unpara...

journal_title：Chromosoma

authors： Matsubara K,O'Meally D,Azad B,Georges A,Sarre SD,Graves JA,Matsuda Y,Ezaz T

更新日期：2016-03-01 00:00:00

abstract：:The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...

journal_title：Chromosoma

authors： Razin SV,Vassetzky YS

更新日期：2017-02-01 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00

abstract：:Allopolyploidy and homoeologous recombination are two important processes in reshaping genomes and generating evolutionary novelties. Newly formed allopolyploids usually display chromosomal perturbations as a result of pairing errors at meiosis. To understand mechanisms of stabilization of allopolyploid species derive...

journal_title：Chromosoma

authors： Wang Y,Zhao Q,Qin X,Yang S,Li Z,Li J,Lou Q,Chen J

更新日期：2017-12-01 00:00:00

abstract：:The preferred positions for meiotic double-strand breakage were mapped on Saccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulse...

journal_title：Chromosoma

authors： Klein S,Zenvirth D,Dror V,Barton AB,Kaback DB,Simchen G

更新日期：1996-12-01 00:00:00

abstract：:We studied the karyotype of specimens of Callithrix emiliae (Callithricidae, Primates) from Rondonia, Brazil. Comparison with the karyotype of Callithrix jacchus showed that, even though these two species show many karyotypic similarities, they differ by a Robertsonian translocation, a paracentric inversion and large-...

journal_title：Chromosoma

authors： de Souza Barros RM,Nagamachi CY,Pieczarka JC

更新日期：1990-10-01 00:00:00

abstract：:The nature of replication origins in eukaryotic chromosomes has been examined in some detail only in yeast, Drosophila, and mammalian cells. We have used highly synchronous cultures of plasmodia of the myxomycete Physarum and two-dimensional agarose gel electrophoresis to examine replication of two developmentally con...

journal_title：Chromosoma

authors： Diller JD,Sauer HW

更新日期：1993-09-01 00:00:00

abstract：:The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...

journal_title：Chromosoma

authors： Ohno M,Fukagawa T,Lee JS,Ikemura T

更新日期：2002-09-01 00:00:00

abstract：:In the domestic pig (2n = 38) two types of constitutive heterochromatin can be differentiated by fluorescence counterstaining techniques. All 24 biarmed autosomes and the X chromosome have chromomycin A3-positive centromeric C-bands, whereas all 12 acrocentric chromosomes exhibit DA-DAPI-positive centromeric heterochr...

journal_title：Chromosoma

authors： Schwarzacher T,Mayr B,Schweizer D

更新日期：1984-01-01 00:00:00

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...

journal_title：Chromosoma

authors： Hancock R

更新日期：2000-07-01 00:00:00

abstract：:The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the fusion orientations of c...

journal_title：Chromosoma

authors： Chi JX,Huang L,Nie W,Wang J,Su B,Yang F

更新日期：2005-08-01 00:00:00

abstract：:The distinctive behaviors of chromosomes in mitosis and meiosis depend upon differences in kinetochore position. Kinetochore position is well established except for a critical transition between meiosis I and meiosis II. We examined kinetochore position during the transition and compared it with the position of kineto...

journal_title：Chromosoma

authors： Paliulis LV,Nicklas RB

更新日期：2005-03-01 00:00:00

abstract：:The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...

journal_title：Chromosoma

authors： Palmer DK,O'Day K,Margolis RL

更新日期：1990-12-01 00:00:00

abstract：:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...

journal_title：Chromosoma

authors： Melnikova L,Kostyuchenko M,Molodina V,Parshikov A,Georgiev P,Golovnin A

更新日期：2018-03-01 00:00:00

abstract：:Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japo...

journal_title：Chromosoma

authors： Svoboda A,Bähler J,Kohli J

更新日期：1995-11-01 00:00:00

abstract：:Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromoso...

journal_title：Chromosoma

authors： Angell RR,Jacobs PA

更新日期：1975-08-11 00:00:00

abstract：:At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We repo...

journal_title：Chromosoma

authors： Moens PB,Earnshaw WC

更新日期：1989-11-01 00:00:00

abstract：:We probed the complex between oriC and DnaA protein using two types of mutants in oriC. Base changes in the DnaA binding sites, DnaA boxes, had little effect on origin function. Mutations which change the distance between DnaA boxes R3 and R4, on the other hand, inactivated oriC unless the mutation deleted or inserted...

journal_title：Chromosoma

authors： Messer W,Hartmann-Kühlein H,Langer U,Mahlow E,Roth A,Schaper S,Urmoneit B,Woelker B

更新日期：1992-01-01 00:00:00

abstract：:P elements, a family of DNA transposons, are known as aggressive intruders into the hitherto uninfected gene pool of Drosophila melanogaster. Invading through horizontal transmission from an external source they managed to spread rapidly through natural populations within a few decades. Owing to their propensity for r...

journal_title：Chromosoma

authors： Pinsker W,Haring E,Hagemann S,Miller WJ

更新日期：2001-07-01 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:Diverse studies indicate that certain nonhistone proteins may be responsible for maintaining the integrity of the eukaryotic chromosomes. In view of their dispersal during nuclear division and their acknowledged role in gene expression, none of the nuclear matrix proteins can be regarded as integral proteins of the ch...

journal_title：Chromosoma

authors： De DN

更新日期：2002-07-01 00:00:00

abstract：:Chromosome segregation ensures the equal partitioning of chromosomes at mitosis. However, long chromosome arms may pose a problem for complete sister chromatid separation. In this paper we report on the analysis of cell division in primary cells from field vole Microtus agrestis, a species with 52 chromosomes includin...

journal_title：Chromosoma

authors： Rens W,Torosantucci L,Degrassi F,Ferguson-Smith MA

更新日期：2006-12-01 00:00:00

abstract：:Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...

journal_title：Chromosoma

authors： Gaeta RT,Masonbrink RE,Zhao C,Sanyal A,Krishnaswamy L,Birchler JA

更新日期：2013-06-01 00:00:00