Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals.

abstract：:We describe a method for preparing nuclear spreads from cells of live, unfixed zebrafish embryos at the late-gastrula (approximately 8000 cell) stage of development. The method consists of a sequence of four steps: (1) a slow, gentle lysis, in low to moderate salt concentration, of cells and then nuclei, to release DN...

journal_title：Chromosoma

authors： Chan DW,Yager TD

更新日期：1998-03-01 00:00:00

abstract：:In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...

journal_title：Chromosoma

authors： Van Hooser AA,Yuh P,Heald R

更新日期：2005-12-01 00:00:00

abstract：:Nucleolar matrix structures were obtained under different extraction conditions from highly purified isolated nucleoli. Their ultrastructural appearance, protein composition and capacity to bind rDNA preferentially were studied in a model binding system. A region spanning approximately 25 kb in the rat ribosomal gene ...

journal_title：Chromosoma

authors： Stephanova E,Stancheva R,Avramova Z

更新日期：1993-03-01 00:00:00

abstract：:Meiotic prophase in Schizosaccharomyces pombe is characterized by striking nuclear movements and the formation of linear elements along chromosomes instead of tripartite synaptonemal complexes. We analysed the organization of nuclei and microtubules in cells of fission yeasts undergoing sexual differentiation. S. japo...

journal_title：Chromosoma

authors： Svoboda A,Bähler J,Kohli J

更新日期：1995-11-01 00:00:00

abstract：:Telomeric DNA is present at the ends of eukaryotic chromosomes and is bound by telomere "capping" proteins, which are the (Cdc13-Stn1-Ten1) CST complex, Ku (Yku70-Yku80), and Rap1-Rif1-Rif2 in budding yeast. Inactivation of any of these complexes causes telomere "uncapping," stimulating a DNA damage response (DDR) tha...

journal_title：Chromosoma

authors： Dewar JM,Lydall D

更新日期：2012-04-01 00:00:00

abstract：:We characterized four meiotic mutants of the fission yeast Schizosaccharomyces pombe by live observation of nuclear movement. Nuclei were stained with either the DNA-specific fluorescent dye Hoechst 33342 or jellyfish green fluorescent protein (GFP) fused with the N-terminal portion of DNA polymerase alpha. We first f...

journal_title：Chromosoma

authors： Hiraoka Y,Ding DQ,Yamamoto A,Tsutsumi C,Chikashige Y

更新日期：2000-01-01 00:00:00

abstract：:The kinetochore binds an evolutionarily conserved set of checkpoint proteins that function to monitor whether chromosomes have aligned properly at the spindle equator. Human cells contain two related protein kinases, hBUB1 and hBUBR1, that appear to have evolved from a single ancestral BUB1 gene. We generated hBUB1- a...

journal_title：Chromosoma

authors： Jablonski SA,Chan GK,Cooke CA,Earnshaw WC,Yen TJ

更新日期：1998-12-01 00:00:00

abstract：:Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...

journal_title：Chromosoma

authors： Gaeta RT,Masonbrink RE,Zhao C,Sanyal A,Krishnaswamy L,Birchler JA

更新日期：2013-06-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

journal_title：Chromosoma

authors： Coelho PA,Nurminsky D,Hartl D,Sunkel CE

更新日期：1996-10-01 00:00:00

abstract：:In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this techniqu...

journal_title：Chromosoma

authors： Liu JY,She CW,Hu ZL,Xiong ZY,Liu LH,Song YC

更新日期：2004-08-01 00:00:00

abstract：:Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication pa...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1992-03-01 00:00:00

abstract：:Replication times for all important chromosome bands, of both types R and Q (277 structures) are analysed. The R-bands form a group of structures whose DNA replicates during the early S-phase, while the DNA situated in the Q-bands replicates during the late S-phase. There may not exist overlapping between replication ...

journal_title：Chromosoma

authors： Dutrillaux B,Couturier J,Richer CL,Viegas-Péquignot E

更新日期：1976-10-12 00:00:00

abstract：:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...

journal_title：Chromosoma

authors： Melnikova L,Kostyuchenko M,Molodina V,Parshikov A,Georgiev P,Golovnin A

更新日期：2018-03-01 00:00:00

abstract：:The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, sever...

journal_title：Chromosoma

authors： Corradini N,Rossi F,Vernì F,Dimitri P

更新日期：2003-07-01 00:00:00

abstract：:With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...

journal_title：Chromosoma

authors： Funaki K,Matsui S,Sasaki M

更新日期：1975-01-01 00:00:00

abstract：:The influence of the sex-reversal factor (Sxr) on X and Y chromosome pairing was examined by comparing males with "novel" and "standard" Sxr genotypes. The novel Sxr males were exceptional in carrying Sxr on their X rather than their Y chromosome, or homozygously on both their X and Y chromosomes, or on a Y chromosome...

journal_title：Chromosoma

authors： Tease C,Cattanach BM

更新日期：1989-03-01 00:00:00

abstract：:We have characterized two post-translational histone modifications in Caenorhabditis elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional a...

journal_title：Chromosoma

authors： Gu SG,Fire A

更新日期：2010-02-01 00:00:00

abstract：:Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...

journal_title：Chromosoma

authors： Rae PM,Barnett T,Murtif VL

更新日期：1981-01-01 00:00:00

abstract：:The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Sat...

journal_title：Chromosoma

authors： Gisselsson D,Shao C,Tuck-Muller CM,Sogorovic S,Pålsson E,Smeets D,Ehrlich M

更新日期：2005-07-01 00:00:00

abstract：:A karyotype based on banding pattern and chromosome length is presented for the white-handed gibbon, Hylobates lar. Little homology with the banding patterns of the chromosomes of the other Hominoidea can be seen, confirming the early evolutionary separation of Hylobatidae and the other apes. Hybridization in situ wit...

journal_title：Chromosoma

authors： Warburton D,Henderson AS,Atwood KC

更新日期：1975-01-01 00:00:00

abstract：:Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused ...

journal_title：Chromosoma

authors： Ansari HA,Maher DW,Pearce PD,Broad TE

更新日期：1996-07-01 00:00:00

abstract：:In mammals, paternal and maternal pronuclei undergo profound chromatin reorganisation upon fertilisation. How these events are orchestrated within centromeric regions to ensure proper chromosome segregation in the following cellular divisions is unknown. In this study, we followed the dynamic unfolding of the centrome...

journal_title：Chromosoma

authors： Probst AV,Santos F,Reik W,Almouzni G,Dean W

更新日期：2007-08-01 00:00:00

abstract：:Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...

journal_title：Chromosoma

authors： Mwathi MW,Schiessl SV,Batley J,Mason AS

更新日期：2019-12-01 00:00:00

abstract：:The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establ...

journal_title：Chromosoma

authors： Sidorenko DS,Sidorenko IA,Zykova TY,Goncharov FP,Larsson J,Zhimulev IF

更新日期：2019-06-01 00:00:00

abstract：:This paper describes the effects of 5-azacytidine on the condensation state of rye (Secale cereale L.) chromatin introduced into the wheat genome (Triticum aestivum L. cv. Beaver). The wheat cultivar Beaver carries a translocation between the short arm of rye chromosome 1R (1RS) and the long arm of wheat chromosome 1B...

journal_title：Chromosoma

authors： Glyn MC,Egertová M,Gazdova B,Kovarik A,Bezdek M,Leitch AR

更新日期：1997-12-01 00:00:00

abstract：:The appearance of RNP droplets associated with specific regions of polytene chromosomes from Chironomus larvae has been studied, systematically by light microscopy using glutarladehyde fixation and lactic acid squashing of the salivary gland. During the fourth larval instar the highest frequency of nuclei which contai...

journal_title：Chromosoma

authors： Stockert JC,Díez JL

更新日期：1979-09-01 00:00:00

abstract：:Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...

journal_title：Chromosoma

authors： Taruscio D,Manuelidis L

更新日期：1991-12-01 00:00:00

abstract：:The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...

journal_title：Chromosoma

authors： Shaw DD

更新日期：1976-02-23 00:00:00

abstract：:During DNA synthesis there is a distinct stage immediately after the joining of large DNA replication intermediates (post-elongation stage). The conversion of this DNA to mature DNA was analysed in cells treated with aphidicolin to stop the movement of the replication fork. In such cells mature DNA is formed. In contr...

journal_title：Chromosoma

authors： Lönn U,Lönn S

更新日期：1987-01-01 00:00:00

abstract：:In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...

journal_title：Chromosoma

authors： Fanti L,Perrini B,Piacentini L,Berloco M,Marchetti E,Palumbo G,Pimpinelli S

更新日期：2008-02-01 00:00:00