Similarities and differences between "uncapped" telomeres and DNA double-strand breaks.

abstract：:The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest ...

journal_title：Chromosoma

authors： Horowitz-Scherer RA,Woodcock CL

更新日期：2006-02-01 00:00:00

abstract：:The frequency and distribution of chiasmata and the nature of terminal "associations" was re-examined in Syrian hamster spermatocytes using the 5-bromo-2'-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the pen...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1990-08-01 00:00:00

abstract：:Formation of stable kinetochore-microtubule attachments is essential for accurate chromosome segregation in human cells and depends on the NDC80 complex. We recently showed that Chmp4c, an endosomal sorting complex required for transport protein involved in membrane remodelling, localises to prometaphase kinetochores ...

journal_title：Chromosoma

authors： Petsalaki E,Dandoulaki M,Zachos G

更新日期：2018-12-01 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:Osteosarcoma (OS) is characterized by chromosomal instability and high copy number gene amplification. The breakage-fusion-bridge (BFB) cycle is a well-established mechanism of genome instability in tumors and in vitro models used to study the origins of complex chromosomal rearrangements and cancer genome amplificati...

journal_title：Chromosoma

authors： Selvarajah S,Yoshimoto M,Park PC,Maire G,Paderova J,Bayani J,Lim G,Al-Romaih K,Squire JA,Zielenska M

更新日期：2006-12-01 00:00:00

abstract：:Chromosome behaviour during meiosis in male Syrian hamsters heterozygous for one of three translocations was analysed as part of a study of the transmission of these structural changes. Synapsis was studied using preparations of synaptonemal complexes, and chiasmate associations and the results of anaphase I segregati...

journal_title：Chromosoma

authors： Cawood AH,Breckon G

更新日期：1989-10-01 00:00:00

abstract：:Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...

journal_title：Chromosoma

authors： Rae PM,Barnett T,Murtif VL

更新日期：1981-01-01 00:00:00

abstract：:Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded state...

journal_title：Chromosoma

authors： Maksimov DA,Koryakov DE,Belyakin SN

更新日期：2014-06-01 00:00:00

abstract：:The vertebrate pore protein Nup153 plays pivotal roles in nuclear pore function. In addition to being important to pore architecture, Nup153 is a key participant in both import and export. The scope of Nup153 function also extends beyond the canonical view of the pore as a trafficking gateway. During the transition in...

journal_title：Chromosoma

authors： Ball JR,Ullman KS

更新日期：2005-11-01 00:00:00

abstract：:A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability....

journal_title：Chromosoma

authors： Tognetti S,Riera A,Speck C

更新日期：2015-03-01 00:00:00

abstract：:Aurora-A is a conserved mitotic kinase overexpressed in many types of cancer. Growing evidence shows that Aurora-A plays a crucial role in DNA damage response (DDR) although this aspect has been less characterized. We isolated a new aur-A mutation, named aur-A949, in Drosophila, and we showed that it causes chromosome...

journal_title：Chromosoma

authors： Merigliano C,Mascolo E,Cesta A,Saggio I,Vernì F

更新日期：2019-03-01 00:00:00

abstract：:PICH (Plk1-interacting checkpoint helicase) was recently identified as an essential component of the spindle assembly checkpoint and shown to localize to kinetochores, inner centromeres, and thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in si...

journal_title：Chromosoma

authors： Wang LH,Schwarzbraun T,Speicher MR,Nigg EA

更新日期：2008-04-01 00:00:00

abstract：:Anaphase lagging of autosomes was observed in 6.1 +/- 5.4% of the primary spermatocytes in untreated larvae of the crane fly, Nephrotoma suturalis. Lagging was induced by exposure of larvae to 6 degrees C and during recovery at 22 degrees C from exposure to 0.2, 2, and 6 degrees C. The incidence of anaphase lag was ma...

journal_title：Chromosoma

authors： Janicke MA,LaFountain JR Jr

更新日期：1982-01-01 00:00:00

abstract：:Mitomycin C-induced sister chromatic exchange and quadriradial formation were studied in chromosomes from Muntiacus muntjac fibroblasts and A9, a transformed mouse cell line. We present the first direct cytological evidence for the induction of true somatic recombination involving homologous chromosomes. Analysis of t...

journal_title：Chromosoma

authors： Huttner KM,Ruddle FH

更新日期：1976-06-30 00:00:00

abstract：:Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...

journal_title：Chromosoma

authors： Yamamoto Y,Gerbi SA

更新日期：2018-12-01 00:00:00

abstract：:The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...

journal_title：Chromosoma

authors： Nonomura K,Kurata N

更新日期：2001-08-01 00:00:00

abstract：:The divergence of lineages leading to extant squamate reptiles (lizards, snakes, and amphisbaenians) and birds occurred about 275 million years ago. Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. Hence, a number of chromosome rearrangements might be ex...

journal_title：Chromosoma

authors： Pokorná M,Giovannotti M,Kratochvíl L,Kasai F,Trifonov VA,O'Brien PC,Caputo V,Olmo E,Ferguson-Smith MA,Rens W

更新日期：2011-10-01 00:00:00

abstract：:By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...

journal_title：Chromosoma

authors： Kato H

更新日期：1977-02-03 00:00:00

abstract：:Studies performed on human trisomic 21 oocytes have revealed that during meiosis, the three homologues 21 synapse and, in some cases, achieve what looks like a trivalent. This implies that meiotic recombination takes place among the three homologous chromosomes 21, and to some extent, crossovers form between them. To ...

journal_title：Chromosoma

authors： Robles P,Roig I,Garcia R,Brieño-Enríquez M,Martin M,Cabero L,Toran N,Garcia Caldés M

更新日期：2013-03-01 00:00:00

abstract：:It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

journal_title：Chromosoma

authors： Elgin SC,Boyd JB

更新日期：1975-01-01 00:00:00

abstract：:The XY bivalent contained within the sex vesicle of chromosomally normal male mice shows virtually no RNA transcriptional activity as measured by autoradiography during the prophase of meiosis. Tertiary trisomic males, derived from the T 70 H and T 31 H reciprocal translocations, include the extra small marker chromos...

journal_title：Chromosoma

authors： Speed RM

更新日期：1986-01-01 00:00:00

abstract：:Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces ...

journal_title：Chromosoma

authors： Foulk MS,Waggener JM,Johnson JM,Yamamoto Y,Liew GM,Urnov FD,Young Y,Lee G,Smith HS,Gerbi SA

更新日期：2013-03-01 00:00:00

abstract：:Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

journal_title：Chromosoma

authors： Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

更新日期：2012-10-01 00:00:00

abstract：:Drosophila melanogaster was transformed with an 18 kb fragment of the C3 DNA puff of Rhynchosciara americana, including the C3-22 gene and the origins of replication that direct amplification. Different tissues and developmental stages of five independent transgenic lines were analyzed by quantitative Southern blot hy...

journal_title：Chromosoma

authors： Soares MA,Monesi N,Basso LR Jr,Stocker AJ,Paçó-Larson ML,Lara FJ

更新日期：2003-10-01 00:00:00

abstract：:Discrete chromatin domains (ChrD), containing an average of approximately 1 Mbp DNA, represent the basic structural units for the regulation of DNA organization and replication in situ. In this study, a bio-computational approach is employed to simultaneously measure the translational motion of large populations of Ch...

journal_title：Chromosoma

authors： Pliss A,Malyavantham K,Bhattacharya S,Zeitz M,Berezney R

更新日期：2009-08-01 00:00:00

abstract：:Repetitive DNA is a major component in most eukaryotic genomes but is ignored in most genome sequencing projects. Here, we report the quantitative composition in repetitive DNA for a supernumerary (B) chromosome, in the migratory locust (Locusta migratoria), by Illumina sequencing of genomic DNA from B-carrying and B-...

journal_title：Chromosoma

authors： Ruiz-Ruano FJ,Cabrero J,López-León MD,Sánchez A,Camacho JPM

更新日期：2018-03-01 00:00:00

abstract：:The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...

journal_title：Chromosoma

authors： Kiknadze II,Perov NA,Chentsov YS

更新日期：1976-03-31 00:00:00

abstract：:By means of a cytological technique involving 5-bromodeoxyuridine, acridine orange, and fluorescence microscopy, the asynchronously replicating, hence genetically inactivated, X chromosome was identified in 6- to 8-day embryos from female mice heterozygous for Searle's translocation T(X;16)16H (abbreviated as T16H) ma...

journal_title：Chromosoma

authors： Takagi N

更新日期：1980-01-01 00:00:00

abstract：:Dioecious Silene latifolia evolved heteromorphic sex chromosomes within the last ten million years, making it a species of choice for studies of the early stages of sex chromosome evolution in plants. About a dozen genes have been isolated from its sex chromosomes and basic genetic and deletion maps exist for the X an...

journal_title：Chromosoma

authors： Howell EC,Armstrong SJ,Filatov DA

更新日期：2011-06-01 00:00:00

abstract：:The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...

journal_title：Chromosoma

authors： Schempp W,Vogel W

更新日期：1978-06-28 00:00:00