Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.

abstract：:DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence micros...

journal_title：Chromosoma

authors： Risley MS,Einheber S,Bumcrot DA

更新日期：1986-01-01 00:00:00

abstract：:Chromosomal replication was studied by means of the BrdU-Höchst-Giemsa-technique in three minute T-cell leukemias with special regard to chromosome 15. In all the three leukemic cell lines the normally early replicating band 15 E was undetectable whereas late replication in this region is completely unaltered. Further...

journal_title：Chromosoma

authors： Somssich IE,Spira J,Hameister H,Klein G

更新日期：1982-01-01 00:00:00

abstract：:A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...

journal_title：Chromosoma

authors： Murata M,Shibata F,Yokota E

更新日期：2006-08-01 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...

journal_title：Chromosoma

authors： Van Hooser AA,Yuh P,Heald R

更新日期：2005-12-01 00:00:00

abstract：:Studies performed on human trisomic 21 oocytes have revealed that during meiosis, the three homologues 21 synapse and, in some cases, achieve what looks like a trivalent. This implies that meiotic recombination takes place among the three homologous chromosomes 21, and to some extent, crossovers form between them. To ...

journal_title：Chromosoma

authors： Robles P,Roig I,Garcia R,Brieño-Enríquez M,Martin M,Cabero L,Toran N,Garcia Caldés M

更新日期：2013-03-01 00:00:00

abstract：:Almost half of the human genome consists of repetitive DNA. Understanding what role these elements have in setting up chromatin states that underlie gene and chromosome function in complex genomes is paramount. The function of some types of repetitive DNA is obvious by virtue of their location, such as the alphoid arr...

journal_title：Chromosoma

authors： Chadwick BP

更新日期：2009-12-01 00:00:00

abstract：:Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...

journal_title：Chromosoma

authors： Cahoon CK,Libuda DE

更新日期：2019-09-01 00:00:00

abstract：:The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...

journal_title：Chromosoma

authors： Dvorkin N,Clark MW,Hamkalo BA

更新日期：1991-09-01 00:00:00

abstract：:Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...

journal_title：Chromosoma

authors： Petcherskaia M,McGuire JM,Pherson JM,Kirk KE

更新日期：2003-04-01 00:00:00

abstract：:Three new 18S.26S rRNA gene loci were identified in common wheat by sequential N-banding and in situ hybridization (ISH) analysis. Locus Nor-A7 is located at the terminal area of the long arm of 5A in both diploid and polyploid wheats. Locus Nor-B6 is located in N-band 1BL2.5 of the long arm of chromosome 1B in Tritic...

journal_title：Chromosoma

authors： Jiang J,Gill BS

更新日期：1994-06-01 00:00:00

abstract：:In a variety of organisms, euchromatic genes brought into juxtaposition with pericentric heterochromatin show position-effect variegation (PEV), a silencing of gene expression in a subset of the cells in which the gene is normally expressed. Previously, a P-element mobilization screen identified transgenic Drosophila ...

journal_title：Chromosoma

authors： Cryderman DE,Cuaycong MH,Elgin SC,Wallrath LL

更新日期：1998-11-01 00:00:00

abstract：:A system has been developed using a microscope photometer to measure the DNA content of individual chromosomes. After correcting for the systematic and statistical errors the measured values are highly reproducible. Data are presented of measurements of human Y chromosomes from a cytogenetically normal population. Whe...

journal_title：Chromosoma

authors： Wall WJ,Butler LJ

更新日期：1989-01-01 00:00:00

abstract：:Telomeric DNA is present at the ends of eukaryotic chromosomes and is bound by telomere "capping" proteins, which are the (Cdc13-Stn1-Ten1) CST complex, Ku (Yku70-Yku80), and Rap1-Rif1-Rif2 in budding yeast. Inactivation of any of these complexes causes telomere "uncapping," stimulating a DNA damage response (DDR) tha...

journal_title：Chromosoma

authors： Dewar JM,Lydall D

更新日期：2012-04-01 00:00:00

abstract：:At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We repo...

journal_title：Chromosoma

authors： Moens PB,Earnshaw WC

更新日期：1989-11-01 00:00:00

abstract：:Previous studies of the Robertsonian polymorphism in the Atlantic dog-whelk, Nucella lapillus (2n = 26-36), have been limited by the inability to identify unequivocally individual chromosomes in the karyotype. This species, as with many other marine invertebrates, has proven largely refractory to the standard (mammali...

journal_title：Chromosoma

authors： Pascoe PL,Patton SJ,Critcher R,Dixon DR

更新日期：1996-03-01 00:00:00

abstract：:Thirty P1 clones from the X chromosome (Muller's A element) of Drosophila melanogaster were cross-hybridized in situ to Drosophila subobscura and Drosophila pseudoobscura polytene chromosomes. An additional recombinant phage lambda Dsuby was also used as a marker. Twenty-three (77%) of the P1 clones gave positive hybr...

journal_title：Chromosoma

authors： Segarra C,Lozovskaya ER,Ribó G,Aguadé M,Hartl DL

更新日期：1995-11-01 00:00:00

abstract：:Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...

journal_title：Chromosoma

authors： Gaeta RT,Masonbrink RE,Zhao C,Sanyal A,Krishnaswamy L,Birchler JA

更新日期：2013-06-01 00:00:00

abstract：:The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...

journal_title：Chromosoma

authors： Palmer DK,O'Day K,Margolis RL

更新日期：1990-12-01 00:00:00

abstract：:Among his many contributions to the field of chromosome structure and dynamics, J. Herbert Taylor showed that eukaryotic cells have many more potential replication origins than they use, which they can recruit when replication forks are slowed to complete S-phase in a timely fashion. Thirty years later, his findings r...

journal_title：Chromosoma

authors： Gilbert DM

更新日期：2007-08-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:The spindle checkpoint restrains anaphase onset and mitotic exit until all chromosomes are stably attached to the mitotic spindle via their kinetochores. The Tao1 protein kinase was recently reported as a novel spindle checkpoint component. When an siRNA was used to repress Tao1, the essential spindle checkpoint compo...

journal_title：Chromosoma

authors： Westhorpe FG,Diez MA,Gurden MD,Tighe A,Taylor SS

更新日期：2010-08-01 00:00:00

abstract：:Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interesting...

journal_title：Chromosoma

authors： Xin H,Zhang T,Han Y,Wu Y,Shi J,Xi M,Jiang J

更新日期：2018-09-01 00:00:00

abstract：:Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...

journal_title：Chromosoma

authors： Morris VB

更新日期：1977-03-16 00:00:00

abstract：:A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...

journal_title：Chromosoma

authors： Satya-Prakash KL,Hsu TC,Pathak S

更新日期：1980-01-01 00:00:00

abstract：:The 1C DNA-content of isolated nuclei of postembryonic stages of Tetranychus urticae stained with the classic and a modified Schiff's reagent was cytophotometrically estimated as 0.1 pg, a low value in animals. For many tissues of this arrhenotokous species the ploidy ratio between males and females is 1:2, indicating...

journal_title：Chromosoma

authors： Tempelaar MJ

更新日期：1980-01-01 00:00:00

abstract：:Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromoso...

journal_title：Chromosoma

authors： Angell RR,Jacobs PA

更新日期：1975-08-11 00:00:00

abstract：:Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

journal_title：Chromosoma

authors： Loidl J,Jin QW,Jantsch M

更新日期：1998-09-01 00:00:00

abstract：:The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, sever...

journal_title：Chromosoma

authors： Corradini N,Rossi F,Vernì F,Dimitri P

更新日期：2003-07-01 00:00:00

abstract：:The genomic DNA organisation patterns of four sauropsidian species, namely Python reticularis, Caiman crocodilus, Terrapene carolina triungius and Columba livia domestica were investigated by reassociation of short and long DNA fragments, by hyperchromicity measurements of reannealed fragments and by length estimation...

journal_title：Chromosoma

authors： Epplen JT,Diedrich U,Wagenmann M,Schmidtke J,Engel W

更新日期：1979-11-01 00:00:00