Ultrastructural localization of nucleic acid sequences in Saccharomyces cerevisiae nucleoli.

abstract：:The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...

journal_title：Chromosoma

authors： Wasserzug-Pash P,Klutstein M

更新日期：2019-09-01 00:00:00

abstract：:Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...

journal_title：Chromosoma

authors： Bähler J,Hagens G,Holzinger G,Scherthan H,Heyer WD

更新日期：1994-04-01 00:00:00

abstract：:In situ hybridization of labeled DNA of four mobile dispersed genetic elements (mdg), isolated from D. melanogaster and C. virilis genomes, with polytene chromosomes of the larvae of several Drosophila species has been carried out. The data show that the mdg elements exhibit a high degree of species specificity. The s...

journal_title：Chromosoma

authors： Evgen'ev MB,Yenikolopov GN,Peunova NI,Ilyin YV

更新日期：1982-01-01 00:00:00

abstract：:Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM,...

journal_title：Chromosoma

authors： Swanton MT,Heumann JM,Prescott DM

更新日期：1980-01-01 00:00:00

abstract：:We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

journal_title：Chromosoma

authors： Lifschytz E,Hareven D

更新日期：1982-01-01 00:00:00

abstract：:At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We repo...

journal_title：Chromosoma

authors： Moens PB,Earnshaw WC

更新日期：1989-11-01 00:00:00

abstract：:In the XY pachytene spermatocyte, the sex chromosomes do not synapse except for the pseudoautosomal region and become transcriptionally silenced. It has been suggested that the meiotic silencing of unsynapsed chromatin (MSUC) also occurs in oocytes. In the XY sex-reversed female mouse, the sex chromosomes fail to pair...

journal_title：Chromosoma

authors： Taketo T,Naumova AK

更新日期：2013-10-01 00:00:00

abstract：:Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

journal_title：Chromosoma

authors： Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

更新日期：2012-10-01 00:00:00

abstract：:Normal lepidopteran males produce two kinds of spermatozoa: nucleate (eupyrene) and anucleate (apyrene). Eupyrene spermatozoa have the usual type of elongate nuclei. But in apyrene spermatids, the nuclei never elongate and the chromatin remains in a telophase-like condition until enucleation occurs. The study of the d...

journal_title：Chromosoma

authors： Friedländer M,Hauschteck-Jungen E

更新日期：1982-01-01 00:00:00

abstract：:In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...

journal_title：Chromosoma

authors： Narayanswami S,Doering JL,Fokta FJ,Rosenthal DS,Nguyen TN,Hamkalo BA

更新日期：1995-10-01 00:00:00

abstract：:Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences we...

journal_title：Chromosoma

authors： Dev VG,Miller DA,Tantravahi R,Schreck RR,Roderick TH,Erlanger BF,Miller OJ

更新日期：1975-12-29 00:00:00

abstract：:Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...

journal_title：Chromosoma

authors： Gaeta RT,Masonbrink RE,Zhao C,Sanyal A,Krishnaswamy L,Birchler JA

更新日期：2013-06-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00

abstract：:We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female c...

journal_title：Chromosoma

authors： Cowell IG,Aucott R,Mahadevaiah SK,Burgoyne PS,Huskisson N,Bongiorni S,Prantera G,Fanti L,Pimpinelli S,Wu R,Gilbert DM,Shi W,Fundele R,Morrison H,Jeppesen P,Singh PB

更新日期：2002-03-01 00:00:00

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

journal_title：Chromosoma

authors： Doerder FP,DeBault LE

更新日期：1978-10-20 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:PICH (Plk1-interacting checkpoint helicase) was recently identified as an essential component of the spindle assembly checkpoint and shown to localize to kinetochores, inner centromeres, and thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in si...

journal_title：Chromosoma

authors： Wang LH,Schwarzbraun T,Speicher MR,Nigg EA

更新日期：2008-04-01 00:00:00

abstract：:Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

journal_title：Chromosoma

authors： LaFountain JR Jr

更新日期：1985-01-01 00:00:00

abstract：:The XY bivalent contained within the sex vesicle of chromosomally normal male mice shows virtually no RNA transcriptional activity as measured by autoradiography during the prophase of meiosis. Tertiary trisomic males, derived from the T 70 H and T 31 H reciprocal translocations, include the extra small marker chromos...

journal_title：Chromosoma

authors： Speed RM

更新日期：1986-01-01 00:00:00

abstract：:In budding yeast, mitotic DNA replication initiates at sequence-specific replication origins, the prototype for which is ARS1. Initiation serves as the primary control point for mitotic DNA replication, and is catalyzed by the Cdc7 protein kinase. In contrast, premeiotic DNA replication apparently does not require Cdc...

journal_title：Chromosoma

authors： Hollingsworth RE Jr,Sclafani RA

更新日期：1993-06-01 00:00:00

abstract：:The Balbiani rings in the salivary gland polytene chromosomes of Chironomus tentans include the most active structural genes in this organ. Two of them (BR1 and BR2) contain repetitive sequences and are transcribed into giant RNA molecules. On Southern blots of restriction digests, we have identified fragments of geno...

journal_title：Chromosoma

authors： Degelmann A,Hollenberg CP

更新日期：1981-01-01 00:00:00

abstract：:One of the prominent cell cycle-related modifications of histone proteins whose function remains unresolved is the phosphorylation of linker histone H1. In this work we have used indirect immunofluorescence on human cells with antibodies that are specific for phosphorylated histone H1 to examine the cellular distribut...

journal_title：Chromosoma

authors： Boggs BA,Allis CD,Chinault AC

更新日期：2000-03-01 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...

journal_title：Chromosoma

authors： Gosden JR,Mitchell AR,Seuanez HN,Gosden CM

更新日期：1977-09-27 00:00:00

abstract：:Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused ...

journal_title：Chromosoma

authors： Ansari HA,Maher DW,Pearce PD,Broad TE

更新日期：1996-07-01 00:00:00

abstract：:Genomic sequencing reveals similar but limited numbers of protein-coding genes in different genomes, which begs the question of how organismal diversities are generated. Alternative pre-mRNA splicing, a widespread phenomenon in higher eukaryotic genomes, is thought to provide a mechanism to increase the complexity of ...

journal_title：Chromosoma

authors： Zhou Z,Fu XD

更新日期：2013-06-01 00:00:00

abstract：:The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest ...

journal_title：Chromosoma

authors： Horowitz-Scherer RA,Woodcock CL

更新日期：2006-02-01 00:00:00

abstract：:The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establ...

journal_title：Chromosoma

authors： Sidorenko DS,Sidorenko IA,Zykova TY,Goncharov FP,Larsson J,Zhimulev IF

更新日期：2019-06-01 00:00:00

abstract：:The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...

journal_title：Chromosoma

authors： Shaw DD,Webb GC,Wilkinson P

更新日期：1976-06-30 00:00:00