The proteins of polytene chromosomes of Drosophila hydei.

Abstract:

:It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important components of constitutive heterochromatin, which is severely underrepresented in polytene chromosomes. Chromosome morphology, including the pattern of induced puffs, is maintained throughout the mass isolation of glands and sucrose gradient purification of nuclei, as indicated by studies on temperature-shocked and control larvae. No significant alteration in the chromosomal proteins following puff induction by heat shock could be detected on analysis of the isolated protein fractions by disc gel electrophoresis. More sensitive techniques must be developed to study the apparent rearrangement or accumulation of protein at puff sites, and to elucidate the role of this protein in gene activation.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Elgin SC,Boyd JB

doi

10.1007/BF00319831

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

135-45

issue

2

eissn

0009-5915

issn

1432-0886

journal_volume

51

pub_type

杂志文章
  • Proportional polyploidization of 5S RNA genes in the ovary of Drosophila melanogaster mutants containing three 5S RNA gene loci.

    abstract::The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328768

    authors: Renkawitz-Pohl R

    更新日期:1979-01-31 00:00:00

  • A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans.

    abstract::The members of the serine-arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle between the nucleus and the cytopl...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-006-0073-5

    authors: Singh OP,Visa N,Wieslander L,Daneholt B

    更新日期:2006-12-01 00:00:00

  • Meiotic pairing and segregation of translocation quadrivalents in yeast.

    abstract::Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050304

    authors: Loidl J,Jin QW,Jantsch M

    更新日期:1998-09-01 00:00:00

  • "Doubled-haploid" allohexaploid Brassica lines lose fertility and viability and accumulate genetic variation due to genomic instability.

    abstract::Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-019-00720-w

    authors: Mwathi MW,Schiessl SV,Batley J,Mason AS

    更新日期:2019-12-01 00:00:00

  • Cytogenetics of triplet conjugation in Tetrahymena: origin of haploid and triploid clones.

    abstract::Triplet conjugation occurs frequently when three different mating types of T. thermophila are mixed under appropriate conditions. Genetic studies show that fertilization is usually tripolar, with each conjugant contributing a pronucleus to one mate and receiving a pronucleus from the other. Triplets are less common an...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00330410

    authors: Preparata RM,Nanney DL

    更新日期:1977-03-07 00:00:00

  • Altered distribution of MLH1 foci is associated with changes in cohesins and chromosome axis compaction in an asynaptic mutant of tomato.

    abstract::In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0363-z

    authors: Qiao H,Offenberg HH,Anderson LK

    更新日期:2012-06-01 00:00:00

  • Partitioning the C. elegans genome by nucleosome modification, occupancy, and positioning.

    abstract::We have characterized two post-translational histone modifications in Caenorhabditis elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional a...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-009-0235-3

    authors: Gu SG,Fire A

    更新日期:2010-02-01 00:00:00

  • The upstreams and downstreams of H3K79 methylation by DOT1L.

    abstract::Histone modifications regulate key processes of eukaryotic genomes. Misregulation of the enzymes that place these modifications can lead to disease. An example of this is DOT1L, the enzyme that can mono-, di-, and trimethylate the nucleosome core on lysine 79 of histone H3 (H3K79). DOT1L plays a role in development an...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-015-0570-5

    authors: Vlaming H,van Leeuwen F

    更新日期:2016-09-01 00:00:00

  • Allocyclic behaviour and underreplication of the nucleolus chromosome in Pseudodiamesa (Chironomidae).

    abstract::The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292473

    authors: Zacharias H

    更新日期:1984-01-01 00:00:00

  • Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides.

    abstract::Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interesting...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-018-0664-y

    authors: Xin H,Zhang T,Han Y,Wu Y,Shi J,Xi M,Jiang J

    更新日期:2018-09-01 00:00:00

  • Cajal's contribution to the knowledge of the neuronal cell nucleus.

    abstract::In 1906, the Spanish neurobiologist Santiago Ramón y Cajal was awarded the Nobel Prize in Physiology or Medicine in recognition of his work on the structure of neurons and their connections. Cajal is commonly regarded as the father of modern neuroscience. What is less well known is that Cajal also had a great interest...

    journal_title:Chromosoma

    pub_type: 传,历史文章,杂志文章,评审

    doi:10.1007/s00412-009-0212-x

    authors: Lafarga M,Casafont I,Bengoechea R,Tapia O,Berciano MT

    更新日期:2009-08-01 00:00:00

  • The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei.

    abstract::The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00337600

    authors: Palmer DK,O'Day K,Margolis RL

    更新日期:1990-12-01 00:00:00

  • Studies on Macronuclear DNA from Paramecium aurelia.

    abstract::Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329171

    authors: Cummings DJ

    更新日期:1975-12-10 00:00:00

  • The diameter of chromatin fibres depends on linker length.

    abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293338

    authors: Alegre C,Subirana JA

    更新日期:1989-06-01 00:00:00

  • Electron microscopic studies on the polytene chromosomes of Chironomus thummi salivary glands. I. Ultrastructural mapping.

    abstract::The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288332

    authors: Kiknadze II,Perov NA,Chentsov YS

    更新日期:1976-03-31 00:00:00

  • Beyond ribosomal DNA: on towards the telomere.

    abstract::We have sequenced and analyzed 8.3 kb of sequence adjacent and distal to the human ribosomal DNA (rDNA); this distal sequence connects to the rDNA cluster just 4 kb upstream of the first promoter and is shared among the acrocentric chromosomes and, at least in part, it is also present in other primates. The sequence d...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF02510479

    authors: Gonzalez IL,Sylvester JE

    更新日期:1997-06-01 00:00:00

  • The 14/15 association as a paradigmatic example of tracing karyotype evolution in New World monkeys.

    abstract::Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-015-0565-2

    authors: Capozzi O,Archidiacono N,Lorusso N,Stanyon R,Rocchi M

    更新日期:2016-09-01 00:00:00

  • Kinetochore rearrangement in meiosis II requires attachment to the spindle.

    abstract::The distinctive behaviors of chromosomes in mitosis and meiosis depend upon differences in kinetochore position. Kinetochore position is well established except for a critical transition between meiosis I and meiosis II. We examined kinetochore position during the transition and compared it with the position of kineto...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-005-0330-z

    authors: Paliulis LV,Nicklas RB

    更新日期:2005-03-01 00:00:00

  • New 18S.26S ribosomal RNA gene loci: chromosomal landmarks for the evolution of polyploid wheats.

    abstract::Three new 18S.26S rRNA gene loci were identified in common wheat by sequential N-banding and in situ hybridization (ISH) analysis. Locus Nor-A7 is located at the terminal area of the long arm of 5A in both diploid and polyploid wheats. Locus Nor-B6 is located in N-band 1BL2.5 of the long arm of chromosome 1B in Tritic...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00368010

    authors: Jiang J,Gill BS

    更新日期:1994-06-01 00:00:00

  • MRE11/RAD50/NBS1: complex activities.

    abstract::The MRE11/RAD50/NBS1 complex (Mre11 complex) is a central player in most aspects of the cellular response to DNA double-strand breaks, including homologous recombination, non-homologous end joining, telomere maintenance and DNA damage checkpoint activation. Several of these findings are explained by the unusual enzyma...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-004-0306-4

    authors: Assenmacher N,Hopfner KP

    更新日期:2004-10-01 00:00:00

  • Evolution of DUX gene macrosatellites in placental mammals.

    abstract::Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0380-y

    authors: Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

    更新日期:2012-10-01 00:00:00

  • Epigenetic changes in mammalian gametes throughout their lifetime: the four seasons metaphor.

    abstract::The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-019-00704-w

    authors: Wasserzug-Pash P,Klutstein M

    更新日期:2019-09-01 00:00:00

  • Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). II. Variation in the pattern of C-banding.

    abstract::The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293114

    authors: Shaw DD,Webb GC,Wilkinson P

    更新日期:1976-06-30 00:00:00

  • Molecular structure of human synaptonemal complex protein SYCE1.

    abstract::The reduction in chromosome number during meiosis is essential for the production of haploid germ cells and thereby fertility. To achieve this, homologous chromosomes are first synapsed together by a protein assembly, the synaptonemal complex (SC), which permits genetic exchange by crossing over and the subsequent acc...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-018-00688-z

    authors: Dunne OM,Davies OR

    更新日期:2019-09-01 00:00:00

  • A new look at the nuclear matrix.

    abstract::The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120000077

    authors: Hancock R

    更新日期:2000-07-01 00:00:00

  • A stringent requirement for Plk1 T210 phosphorylation during K-fiber assembly and chromosome congression.

    abstract::Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain contro...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0375-8

    authors: Paschal CR,Maciejowski J,Jallepalli PV

    更新日期:2012-12-01 00:00:00

  • Distribution of 18+28S ribosomal genes in mammalian genomes.

    abstract::In situ hybridization with 3H 18S and 28S ribosomal RNA from Xenopus laevis has been used to study the distribution of DNA sequences coding for these RNAs (the nucleolus organizing regions) in the genomes of six mammals. Several patterns of distribution have been found: 1) A single major site (rat kangaroo, Seba's fru...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329388

    authors: Hsu TC,Spirito SE,Pardue ML

    更新日期:1975-11-20 00:00:00

  • The influence of 5-azacytidine on the condensation of the short arm of rye chromosome 1R in Triticum aestivum L. root tip meristematic nuclei.

    abstract::This paper describes the effects of 5-azacytidine on the condensation state of rye (Secale cereale L.) chromatin introduced into the wheat genome (Triticum aestivum L. cv. Beaver). The wheat cultivar Beaver carries a translocation between the short arm of rye chromosome 1R (1RS) and the long arm of wheat chromosome 1B...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/pl00007688

    authors: Glyn MC,Egertová M,Gazdova B,Kovarik A,Bezdek M,Leitch AR

    更新日期:1997-12-01 00:00:00

  • Molecular cloning of zebra finch W chromosome repetitive sequences: evolution of the avian W chromosome.

    abstract::The zebra finch (Taeniopygia guttata) has a large Z chromosome and highly condensed W chromosome. We used the random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique to isolate female-specific sequences ZBM1 and ZBM2. Southern blot hybridization to male and female zebra finch genomic DNA sugg...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-007-0130-8

    authors: Itoh Y,Kampf K,Arnold AP

    更新日期:2008-04-01 00:00:00

  • Linking replication stress with heterochromatin formation.

    abstract::The eukaryotic genome can be roughly divided into euchromatin and heterochromatin domains that are structurally and functionally distinct. Heterochromatin is characterized by its high compaction that impedes DNA transactions such as gene transcription, replication, or recombination. Beyond its role in regulating DNA a...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-015-0545-6

    authors: Nikolov I,Taddei A

    更新日期:2016-06-01 00:00:00