Abstract:
:Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain controversial. Using gene targeting, we replaced the native PLK1 locus in human cells with either PLK1 (T210A) or PLK1 (T210D) in both dominant and recessive settings. In contrast to previous reports, PLK1 (T210D) did not accelerate cells prematurely into mitosis, nor could it fulfill the kinase's essential role in chromosome congression. The latter was traced to an unexpected defect in Plk1-dependent phosphorylation of BubR1, a key mediator of stable kinetochore-microtubule attachment. Using chemical genetics to bypass this defect, we found that Plk1(T210D) is nonetheless able to induce equatorial RhoA zones and cleavage furrows during mitotic exit. Collectively, our data indicate that K-fibers are sensitive to even subtle perturbations in T210 phosphorylation and caution against relying on Plk1(T210D) as an in vivo surrogate for the natively activated kinase.
journal_name
Chromosomajournal_title
Chromosomaauthors
Paschal CR,Maciejowski J,Jallepalli PVdoi
10.1007/s00412-012-0375-8subject
Has Abstractpub_date
2012-12-01 00:00:00pages
565-72issue
6eissn
0009-5915issn
1432-0886journal_volume
121pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences we...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294081
更新日期:1975-12-29 00:00:00
abstract::When A-banded human chromosomes mounted in water are transferred to an organic mounting medium, the chromosomes show uniform bright quinacrine fluorescence. This change is reversible. It is inferred that quinacrine is bound uniformly along the chromosomes, and that Q-banding is a consequence of a non-uniform distribut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294941
更新日期:1977-12-06 00:00:00
abstract::The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-014-0479-4
更新日期:2014-12-01 00:00:00
abstract::Aedes aegypti spermatocytes were reconstructed from electron micrographs. The species has tight somatic pairing of the chromosomes, and there are therefore no classical leptotene and zygotene stages, but rather a gradual transition from somatic pairing to meiotic pairing (= pachytene). The term "prepachytene" has been...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329808
更新日期:1985-01-01 00:00:00
abstract::Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-018-0677-6
更新日期:2018-12-01 00:00:00
abstract::The chromosomes of lake trout (Salvelinus namaycush) contain a considerable amount of heterochromatin located at the centromeres and/or telomeres of several chromosomes, including a sex-specific block located distally on the X chromosome. In order to investigate further the repetitive DNAs of lake trout, genomic DNA f...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352255
更新日期:1995-12-01 00:00:00
abstract::Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00692-x
更新日期:2019-09-01 00:00:00
abstract::The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degene...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-006-0086-0
更新日期:2007-04-01 00:00:00
abstract::Metaphase chromosomes prepared from colcemid-treated mouse L929 cells by non-ionic detergent lysis exhibit distinct heterochromatic centromere regions and associated kinetochores when viewed by whole mount electron microscopy. Deoxyribonuclease I treatment of these chromosomes results in the preferential digestion of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330554
更新日期:1978-04-25 00:00:00
abstract::High resolution patterns of DNA replications in human lymphocyte chromosomes during early and late S-phases were studied by means of the BrdU-Hoechst-Giemsa technique. The late replicating bands were found to be identical with highly detailed G-bands. Between early replicating bands and R-bands subtile differences wer...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286114
更新日期:1981-01-01 00:00:00
abstract::Minute elements detected in Megaselia scalaris (Phoridae, Diptera) lack chromosome arms but carry centromeres and possess kinetochore microtubules in mitosis as well as in meiosis. These centromere-like elements (CLEs) were present in two geographically independent strains of the fly. This indicates that their origin ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00357059
更新日期:1991-11-01 00:00:00
abstract::Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294845
更新日期:1979-06-21 00:00:00
abstract::Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050343
更新日期:1998-12-01 00:00:00
abstract::Osteosarcoma (OS) is characterized by chromosomal instability and high copy number gene amplification. The breakage-fusion-bridge (BFB) cycle is a well-established mechanism of genome instability in tumors and in vitro models used to study the origins of complex chromosomal rearrangements and cancer genome amplificati...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-006-0074-4
更新日期:2006-12-01 00:00:00
abstract::The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352253
更新日期:1994-07-01 00:00:00
abstract::We report on the construction of the first comparative Zoo-FISH map of a marine mammal. Zoo-FISH with DNA probes from a human chromosome-specific library to metaphase spreads of the harbor seal (Phoca vitulina) disclosed 31 conserved syntenic segments covering the complete autosomal complement and the X chromosome. Co...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050230
更新日期:1997-07-01 00:00:00
abstract::Rice (Oryza sativa L.) centromeres are composed of 155-bp satellite repeats (CentO), centromere-specific retrotransposon (CRR), and a variety of other repeats. Previous studies have shown that CentO and CRR elements are both parts of the functional centromere/kinetochore complex. In this study, a naturally occurring k...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0413-1
更新日期:2013-08-01 00:00:00
abstract::Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0403-3
更新日期:2013-06-01 00:00:00
abstract::Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM,...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329546
更新日期:1980-01-01 00:00:00
abstract::The DNA of eukaryotic cells is organized in a complex with proteins, either as interphase chromatin or mitotic chromosomes. Nucleosomes, the structural subunits of chromatin, have long been considered as static structures, incompatible with processes occurring in chromatin. During the past few years it has become evid...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/BF00337382
更新日期:1994-12-01 00:00:00
abstract::Of 204 mutations located in the 8-12 band Df(2L)130 region, 37B9-C1,2;37D1-2, 199 have been assigned to twelve lethal genes and one visible gene (hook). The 13 genes are not evenly distributed. Twelve, (possibly all thirteen) are in the seven band region 37B10-C4 giving a gene-to-band ratio of almost two. Only one gen...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286015
更新日期:1981-01-01 00:00:00
abstract::Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-016-0596-3
更新日期:2017-03-01 00:00:00
abstract::DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence micros...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288496
更新日期:1986-01-01 00:00:00
abstract::We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330119
更新日期:1982-01-01 00:00:00
abstract::The distribution patterns of chromosomal proteins from Drosophila can be observed by immunofluorescent staining of the polytene chromosomes from larval salivary glands. We have purified a non-histone chromosomal protein of Mr = 69,000 molecular weight which has a high affinity for DNA with little sequence specificity....
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285889
更新日期:1987-01-01 00:00:00
abstract::With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285128
更新日期:1975-01-01 00:00:00
abstract::Two families of tandemly repeated satellite DNAs were isolated from the neotropical primate Callimico goeldii (Goeldi's marmoset). One satellite, CgoA, is over 70% A + T and has a monomer length of 338 bp. The other satellite, CgoB, is 50% A + T and has a monomer length of 916 bp. Both CgoA and CgoB hybridize strongly...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292784
更新日期:1989-12-01 00:00:00
abstract::The distribution of quinacrine and protein sulphur has been compared with that of DNA in euchromatic and heterochromatic regions of mouse chromosomes stained with the fluorescent dye quinacrine, using X-ray microanalysis. Heterochromatin tends to bind relatively more quinacrine than euchromatin, and contains a greater...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294059
更新日期:1985-01-01 00:00:00
abstract::We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is charac...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01731720
更新日期:1990-09-01 00:00:00
abstract::The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-017-0645-6
更新日期:2018-03-01 00:00:00