Abstract：
:The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. With fluorescence in situ hybridization (FISH), the euchromatin probe labelled all chromosomes, whereas the W-chromatin DNA proved to be a highly specific W-chromosome painting probe. For sequence analysis, DOP-PCR-generated DNA fragments were cloned, sequenced, and tested by Southern hybridization. We recovered single-copy and low-copy W-specific sequences, a sequence that was located only in the W and the Z chromosome, multi-copy sequences that were enriched in the W chromosome but occurred also elsewhere, and ubiquitous multi-copy sequences. Three of the multi-copy sequences were recognized as derived from hitherto unknown retrotransposons. The results show that our approach is feasible and that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism. The W chromosome has attracted repetitive sequences during evolution but also contains unique sequences.
journal_name
Chromosomajournal_title
Chromosomaauthors
Fuková I,Traut W,Vítková M,Nguyen P,Kubícková S,Marec Fdoi
10.1007/s00412-006-0086-0subject
Has Abstractpub_date
2007-04-01 00:00:00pages
135-45issue
2eissn
0009-5915issn
1432-0886journal_volume
116pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract：:A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-005-0045-1
更新日期：2006-08-01 00:00:00
abstract：:Heterochromatin in eukaryotic interphase cells frequently localizes to the nucleolar periphery (nucleolus-associated domains (NADs)) and the nuclear lamina (lamina-associated domains (LADs)). Gene expression in somatic cell NADs is generally low, but NADs have not been characterized in mammalian stem cells. Here, we g...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-020-00734-9
更新日期：2020-06-01 00:00:00
abstract：:In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-007-0123-7
更新日期：2008-02-01 00:00:00
abstract：:Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00291004
更新日期：1985-01-01 00:00:00
abstract：:The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-019-00704-w
更新日期：2019-09-01 00:00:00
abstract：:In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this techniqu...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-004-0289-1
更新日期：2004-08-01 00:00:00
abstract：:The sex chromosomes in Sauropsida (reptiles and birds) have evolved independently many times. They show astonishing diversity in morphology ranging from cryptic to highly differentiated sex chromosomes with male (XX/XY) and female heterogamety (ZZ/ZW). Comparing such diverse sex chromosome systems thus provides unpara...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-015-0531-z
更新日期：2016-03-01 00:00:00
abstract：:Studies performed on human trisomic 21 oocytes have revealed that during meiosis, the three homologues 21 synapse and, in some cases, achieve what looks like a trivalent. This implies that meiotic recombination takes place among the three homologous chromosomes 21, and to some extent, crossovers form between them. To ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-012-0394-5
更新日期：2013-03-01 00:00:00
abstract：:The 1C DNA-content of isolated nuclei of postembryonic stages of Tetranychus urticae stained with the classic and a modified Schiff's reagent was cytophotometrically estimated as 0.1 pg, a low value in animals. For many tissues of this arrhenotokous species the ploidy ratio between males and females is 1:2, indicating...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00286060
更新日期：1980-01-01 00:00:00
abstract：:The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292473
更新日期：1984-01-01 00:00:00
abstract：:A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292418
更新日期：1980-01-01 00:00:00
abstract：:We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00365153
更新日期：1991-12-01 00:00:00
abstract：:In mammals, paternal and maternal pronuclei undergo profound chromatin reorganisation upon fertilisation. How these events are orchestrated within centromeric regions to ensure proper chromosome segregation in the following cellular divisions is unknown. In this study, we followed the dynamic unfolding of the centrome...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-007-0106-8
更新日期：2007-08-01 00:00:00
abstract：:Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-019-00720-w
更新日期：2019-12-01 00:00:00
abstract：:Micronuclei are extra-nuclear bodies mainly derived from ana-telophase lagging chromosomes/chromatins (LCs) that are not incorporated into primary nuclei at mitotic exit. Unlike primary nuclei, most micronuclei are enclosed by nuclear envelope (NE) that is highly susceptible to spontaneous and irreparable rupture. Rup...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-020-00741-w
更新日期：2020-12-01 00:00:00
abstract：:Normal lepidopteran males produce two kinds of spermatozoa: nucleate (eupyrene) and anucleate (apyrene). Eupyrene spermatozoa have the usual type of elongate nuclei. But in apyrene spermatids, the nuclei never elongate and the chromatin remains in a telophase-like condition until enucleation occurs. The study of the d...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00330361
更新日期：1982-01-01 00:00:00
abstract：:Homologous chromosomes exchange genetic information through recombination during meiosis, a process that increases genetic diversity, and is fundamental to sexual reproduction. In an attempt to shed light on the dynamics of mammalian recombination and its implications for genome organization, we have studied the recom...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-016-0624-3
更新日期：2017-10-01 00:00:00
abstract：:The chromosomes of lake trout (Salvelinus namaycush) contain a considerable amount of heterochromatin located at the centromeres and/or telomeres of several chromosomes, including a sex-specific block located distally on the X chromosome. In order to investigate further the repetitive DNAs of lake trout, genomic DNA f...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00352255
更新日期：1995-12-01 00:00:00
abstract：:With simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifie...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00362284
更新日期：1994-10-01 00:00:00
abstract：:The location of DNA fragments representing mobile dispersed genes (MDG) in salivary gland and midgut polytene chromosomes was compared by means of in situ hybridization. In the Drosophila stock under study the average number of hybridization sites in the polytene chromosomes of one nucleus was 20 for MDG-1 and 10 for ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00285767
更新日期：1981-01-01 00:00:00
abstract：:Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-009-0239-z
更新日期：2010-02-01 00:00:00
abstract：:We describe a mammalian artificial mini-chromosome lacking human alphoid DNA and mouse minor and major satellite DNA repeats. This mini-chromosome, initially recovered in a mouse embryonic stem (ES) cell line (CGR8), is 2.6 Mb in size and consists of sequences derived from the human Y chromosome and mouse chromosomes ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s004120100160
更新日期：2001-11-01 00:00:00
abstract：:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-017-0645-6
更新日期：2018-03-01 00:00:00
abstract：:The ribosomal DNA(rDNA)-containing chromatin in eukaryotes forms a unique architecture called the "secondary constriction" or "nucleolus organiser region (NOR)" on mitotic chromosomes. To gain more insight into non-histone chromosomal proteins (NHCP), termed "N-band proteins", that are specifically associated with the...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292743
更新日期：1986-01-01 00:00:00
abstract：:The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, sever...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-003-0241-9
更新日期：2003-07-01 00:00:00
abstract：:Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-006-0087-z
更新日期：2007-04-01 00:00:00
abstract：:We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is charac...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF01731720
更新日期：1990-09-01 00:00:00
abstract：:Facioscapulohumeral muscular dystrophy (FSHD) is caused by the shortening of a copy-number polymorphic array of 3.3 kb repeats (D4Z4) at one allelic 4q35.2 region. How this contraction of a subtelomeric tandem array causes FSHD is unknown but indirect evidence suggests that a short array has a cis effect on a distant ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-004-0280-x
更新日期：2004-05-01 00:00:00
abstract：:We provide evidence that centromere-specific 155 bp DNA repeats terminate one pair of telomeres at the telocentric, left end of the short fourth chromosome in Chironomus pallidivittatus. Earlier evidence indicated that all other telomeres are terminated by 340 bp telomere-specific repeats. DNA that borders the 155 bp ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-001-0176-y
更新日期：2002-02-01 00:00:00
abstract：:The eukaryotic genome can be roughly divided into euchromatin and heterochromatin domains that are structurally and functionally distinct. Heterochromatin is characterized by its high compaction that impedes DNA transactions such as gene transcription, replication, or recombination. Beyond its role in regulating DNA a...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-015-0545-6
更新日期：2016-06-01 00:00:00
