Abstract：
:With simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifies unique sequences and a 720 kb yeast artificial chromosome (YAC) probe that recognizes a specific region of the chromatin domain. The heterologous DNA consists of a 1.8 Mb insertion of 40 tandem head-to-tail phage lambda LIZ vectors and of 11.4 Mb of bacterial/mouse DNA repeats. The lengthy lambda insert is unusual in that it is not contained in the chromatin domain of chromosome 4 and in that it fails to form direct attachments to the chromosome core. The ends are attached indirectly, probably by means of the flanking mouse sequences. At late stages of meiotic prophase, while the terminal attachments remain the same, the lambda DNA becomes highly compacted. Apparently, higher order condensation and core attachment are independent processes. The condensed inserts relax precociously at metaphase I. In the mouse heterozygous for the insert, the two sister inserts are usually merged, as are all four inserts in the homozygous mouse. Evidently chromatin loops with identical sequences can become associated during meiotic prophase. Mouse sequences within a heterologous DNA insert (repeats of bacterial plasmid pBR322 with a mouse beta-globin insert) were observed to restore some degree of core attachment.
journal_name
Chromosomajournal_title
Chromosomaauthors
Heng HH,Tsui LC,Moens PBdoi
10.1007/BF00362284subject
Has Abstractpub_date
1994-10-01 00:00:00pages
401-7issue
6eissn
0009-5915issn
1432-0886journal_volume
103pub_type
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