Abstract:
:We describe a mammalian artificial mini-chromosome lacking human alphoid DNA and mouse minor and major satellite DNA repeats. This mini-chromosome, initially recovered in a mouse embryonic stem (ES) cell line (CGR8), is 2.6 Mb in size and consists of sequences derived from the human Y chromosome and mouse chromosomes 12 and 15. It is not stable in the CGR8 cells but replicates and segregates with high fidelity after transfer into chicken DT40 cells. Combined analysis by immunocytochemistry/fluorescence in situ hybridisation (FISH) on metaphase spreads detected an active neo-centromere on the mini-chromosome in these cells. Further analysis by immunocytochemistry/FISH on stretched chromatin allowed the localisation of the CENP-C protein to the DNA sequence derived from interval 5 of the human Y chromosome.
journal_name
Chromosomajournal_title
Chromosomaauthors
Shen MH,Ross A,Yang J,de las Heras JI,Cooke Hdoi
10.1007/s004120100160subject
Has Abstractpub_date
2001-11-01 00:00:00pages
421-9issue
6eissn
0009-5915issn
1432-0886journal_volume
110pub_type
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