Deposition, turnover, and release of CENH3 at Arabidopsis centromeres.

abstract：:This investigation was conducted to determine the relationship between Y chromosome loss and increased micronucleus formation with age. We also investigated the status of kinetochore proteins in the micronuclei. Umbilical cord blood samples were obtained from 18 newborn males, and peripheral blood was obtained from 35...

journal_title：Chromosoma

authors： Nath J,Tucker JD,Hando JC

更新日期：1995-07-01 00:00:00

abstract：:MicroRNAs (miRNAs) were first discovered in genetic screens for regulators of developmental timing in the stem-cell-like seam cell lineage in Caenorhabditis elegans. As members of the heterochronic pathway, the lin-4 and let-7 miRNAs are required in the seam cells for the correct progression of stage-specific events a...

journal_title：Chromosoma

authors： Nimmo RA,Slack FJ

更新日期：2009-08-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

journal_title：Chromosoma

authors： Coelho PA,Nurminsky D,Hartl D,Sunkel CE

更新日期：1996-10-01 00:00:00

abstract：:A higher order organization of the centromeres in the form of clustering of these DNA loci has been observed in many organisms. While centromere clustering is biologically significant to achieve faithful chromosome segregation, the underlying molecular mechanism is yet to be fully understood. In budding yeast, a kinet...

journal_title：Chromosoma

authors： Mittal P,Chavan A,Trakroo D,Shah S,Ghosh SK

更新日期：2019-06-01 00:00:00

abstract：:The irradiation of metaphase spreads of human cells with ultraviolet (UV) light blocked the chromosome banding induced by Alu I, Mbo I, Dde I, Hinf I, Hae III, and Rsa I restriction endonucleases. At 13 J/m2 there was moderate inhibition of the nuclease action, which was detected as an increase in the stain intensity ...

journal_title：Chromosoma

authors： Bianchi NO,Bianchi MS,Cleaver JE

更新日期：1984-01-01 00:00:00

abstract：:Cyclins, as regulatory partners of cyclin-dependent kinases (CDKs), control the switch-like cell cycle transitions that orchestrate orderly duplication and segregation of genomes. Compared to mitosis, relatively little is known about how cyclin-CDK complexes control meiosis, the specialized cell division that generate...

journal_title：Chromosoma

authors： Karasu ME,Keeney S

更新日期：2019-09-01 00:00:00

abstract：:The role of helix 6, which forms the major portion of the most 5'-located expansion segment of Saccharomyces cerevisiae 18S rRNA, was studied by in vivo mutational analysis. Mutations that increased the size of the helical part and/or the loop, even to a relatively small extent, abolished 18S rRNA formation almost com...

journal_title：Chromosoma

authors： van Nues RW,Venema J,Planta RJ,Raué HA

更新日期：1997-06-01 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00

abstract：:Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

journal_title：Chromosoma

authors： Kavenoff R,Bowen BC

更新日期：1976-12-16 00:00:00

abstract：:We describe a method for preparing nuclear spreads from cells of live, unfixed zebrafish embryos at the late-gastrula (approximately 8000 cell) stage of development. The method consists of a sequence of four steps: (1) a slow, gentle lysis, in low to moderate salt concentration, of cells and then nuclei, to release DN...

journal_title：Chromosoma

authors： Chan DW,Yager TD

更新日期：1998-03-01 00:00:00

abstract：:The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X c...

journal_title：Chromosoma

authors： Loebel DA,Johnston PG

更新日期：1993-01-01 00:00:00

abstract：:Rice (Oryza sativa L.) centromeres are composed of 155-bp satellite repeats (CentO), centromere-specific retrotransposon (CRR), and a variety of other repeats. Previous studies have shown that CentO and CRR elements are both parts of the functional centromere/kinetochore complex. In this study, a naturally occurring k...

journal_title：Chromosoma

authors： Wang G,Li H,Cheng Z,Jin W

更新日期：2013-08-01 00:00:00

abstract：:With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...

journal_title：Chromosoma

authors： Funaki K,Matsui S,Sasaki M

更新日期：1975-01-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe...

journal_title：Chromosoma

authors： Golic MM,Golic KG

更新日期：2011-04-01 00:00:00

abstract：:Osteosarcoma (OS) is characterized by chromosomal instability and high copy number gene amplification. The breakage-fusion-bridge (BFB) cycle is a well-established mechanism of genome instability in tumors and in vitro models used to study the origins of complex chromosomal rearrangements and cancer genome amplificati...

journal_title：Chromosoma

authors： Selvarajah S,Yoshimoto M,Park PC,Maire G,Paderova J,Bayani J,Lim G,Al-Romaih K,Squire JA,Zielenska M

更新日期：2006-12-01 00:00:00

abstract：:Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

journal_title：Chromosoma

authors： LaFountain JR Jr

更新日期：1985-01-01 00:00:00

abstract：:The appearance of RNP droplets associated with specific regions of polytene chromosomes from Chironomus larvae has been studied, systematically by light microscopy using glutarladehyde fixation and lactic acid squashing of the salivary gland. During the fourth larval instar the highest frequency of nuclei which contai...

journal_title：Chromosoma

authors： Stockert JC,Díez JL

更新日期：1979-09-01 00:00:00

abstract：:Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

journal_title：Chromosoma

authors： Loidl J,Jin QW,Jantsch M

更新日期：1998-09-01 00:00:00

abstract：:To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...

journal_title：Chromosoma

authors： Maeshima K,Eltsov M,Laemmli UK

更新日期：2005-11-01 00:00:00

abstract：:The distribution patterns of chromosomal proteins from Drosophila can be observed by immunofluorescent staining of the polytene chromosomes from larval salivary glands. We have purified a non-histone chromosomal protein of Mr = 69,000 molecular weight which has a high affinity for DNA with little sequence specificity....

journal_title：Chromosoma

authors： Fleischmann G,Filipski R,Elgin SC

更新日期：1987-01-01 00:00:00

abstract：:Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...

journal_title：Chromosoma

authors： Han Y,Wang G,Liu Z,Liu J,Yue W,Song R,Zhang X,Jin W

更新日期：2010-02-01 00:00:00

abstract：:The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...

journal_title：Chromosoma

authors： Palmer DK,O'Day K,Margolis RL

更新日期：1990-12-01 00:00:00

abstract：:During early meiotic development the yeast Saccharomyces cerevisiae has a characteristic nuclear dense body (NDB). It is shown that the NDB can also be induced in vegetatively growing cells through the inhibition of thymidylate synthetase which causes depletion of the dTMP pool and arrests DNA synthesis. The observati...

journal_title：Chromosoma

authors： Moens PB,Barclay BJ,Little JG

更新日期：1981-01-01 00:00:00

abstract：:The genomic DNA organisation patterns of four sauropsidian species, namely Python reticularis, Caiman crocodilus, Terrapene carolina triungius and Columba livia domestica were investigated by reassociation of short and long DNA fragments, by hyperchromicity measurements of reannealed fragments and by length estimation...

journal_title：Chromosoma

authors： Epplen JT,Diedrich U,Wagenmann M,Schmidtke J,Engel W

更新日期：1979-11-01 00:00:00

abstract：:By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...

journal_title：Chromosoma

authors： Kato H

更新日期：1977-02-03 00:00:00

abstract：:We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

journal_title：Chromosoma

authors： Lifschytz E,Hareven D

更新日期：1982-01-01 00:00:00

abstract：:Dioecious Silene latifolia evolved heteromorphic sex chromosomes within the last ten million years, making it a species of choice for studies of the early stages of sex chromosome evolution in plants. About a dozen genes have been isolated from its sex chromosomes and basic genetic and deletion maps exist for the X an...

journal_title：Chromosoma

authors： Howell EC,Armstrong SJ,Filatov DA

更新日期：2011-06-01 00:00:00

abstract：:BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment...

journal_title：Chromosoma

authors： Takayama S,Utsumi KR,Sasaki Y

更新日期：1981-01-01 00:00:00

abstract：:Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...

journal_title：Chromosoma

authors： Melnikova L,Shapovalov I,Kostyuchenko M,Georgiev P,Golovnin A

更新日期：2017-03-01 00:00:00