Nuclear morphology of yeast under thymidylate starvation.

abstract：:The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...

journal_title：Chromosoma

authors： Renkawitz-Pohl R

更新日期：1979-01-31 00:00:00

abstract：:Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...

journal_title：Chromosoma

authors： Howell WM,Hsu TC

更新日期：1979-06-21 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00

abstract：:DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Triturus cristatus (23 pg) and Necturus maculosus (52 pg) of the ...

journal_title：Chromosoma

authors： Baldari CT,Amaldi F

更新日期：1976-12-06 00:00:00

abstract：:The frequency and distribution of chiasmata and the nature of terminal "associations" was re-examined in Syrian hamster spermatocytes using the 5-bromo-2'-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the pen...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1990-08-01 00:00:00

abstract：:In Drosophila as in many organisms beta tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the beta 1 and beta 2 tubulins of Drosophila melanogaster and the beta 2 tubulin of D. hydei, and found these insect beta tubulins to be highly conserved and like beta tubulins...

journal_title：Chromosoma

authors： Michiels F,Falkenburg D,Müller AM,Hinz U,Otto U,Bellmann R,Glätzer KH,Brand R,Bialojan S,Renkawitz-Pohl R

更新日期：1987-01-01 00:00:00

abstract：:A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...

journal_title：Chromosoma

authors： Satya-Prakash KL,Hsu TC,Pathak S

更新日期：1980-01-01 00:00:00

abstract：:Antitubulin antibody was used as an immunofluorescent and immunoelectron microscopic probe to localize tubulin in components of the mitotic apparatus of rat kangaroo (strain PtK1) cells in vitro. In addition to the detection of tubulin in the spindle microtubules and centrioles, other structures were found to display ...

journal_title：Chromosoma

authors： Pepper DA,Brinkley BR

更新日期：1977-04-19 00:00:00

abstract：:The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degene...

journal_title：Chromosoma

authors： Fuková I,Traut W,Vítková M,Nguyen P,Kubícková S,Marec F

更新日期：2007-04-01 00:00:00

abstract：:Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...

journal_title：Chromosoma

authors： Gosden JR,Mitchell AR,Seuanez HN,Gosden CM

更新日期：1977-09-27 00:00:00

abstract：:The preferred positions for meiotic double-strand breakage were mapped on Saccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulse...

journal_title：Chromosoma

authors： Klein S,Zenvirth D,Dror V,Barton AB,Kaback DB,Simchen G

更新日期：1996-12-01 00:00:00

abstract：:Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...

journal_title：Chromosoma

authors： Turner BM,Keohane A

更新日期：1987-01-01 00:00:00

abstract：:Recent progress in elucidating the function of synaptonemal complex (SC) proteins and of cohesins in meiocytes made possible, in particular, through the analysis of mice deficient in SC or cohesin proteins has significantly enriched our understanding of how meiotic chromosome architecture is determined. Cohesins and t...

journal_title：Chromosoma

authors： Revenkova E,Jessberger R

更新日期：2006-06-01 00:00:00

abstract：:Cyclins, as regulatory partners of cyclin-dependent kinases (CDKs), control the switch-like cell cycle transitions that orchestrate orderly duplication and segregation of genomes. Compared to mitosis, relatively little is known about how cyclin-CDK complexes control meiosis, the specialized cell division that generate...

journal_title：Chromosoma

authors： Karasu ME,Keeney S

更新日期：2019-09-01 00:00:00

abstract：:The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...

journal_title：Chromosoma

authors： Ohno M,Fukagawa T,Lee JS,Ikemura T

更新日期：2002-09-01 00:00:00

abstract：:We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...

journal_title：Chromosoma

authors： Kress H,Meyerowitz EM,Davidson N

更新日期：1985-01-01 00:00:00

abstract：:Diverse studies indicate that certain nonhistone proteins may be responsible for maintaining the integrity of the eukaryotic chromosomes. In view of their dispersal during nuclear division and their acknowledged role in gene expression, none of the nuclear matrix proteins can be regarded as integral proteins of the ch...

journal_title：Chromosoma

authors： De DN

更新日期：2002-07-01 00:00:00

abstract：:A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...

journal_title：Chromosoma

authors： Murata M,Shibata F,Yokota E

更新日期：2006-08-01 00:00:00

abstract：:The zebra finch (Taeniopygia guttata) has a large Z chromosome and highly condensed W chromosome. We used the random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique to isolate female-specific sequences ZBM1 and ZBM2. Southern blot hybridization to male and female zebra finch genomic DNA sugg...

journal_title：Chromosoma

authors： Itoh Y,Kampf K,Arnold AP

更新日期：2008-04-01 00:00:00

abstract：:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...

journal_title：Chromosoma

authors： Schäfer R,Ali S,Epplen JT

更新日期：1986-01-01 00:00:00

abstract：:Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...

journal_title：Chromosoma

authors： Petcherskaia M,McGuire JM,Pherson JM,Kirk KE

更新日期：2003-04-01 00:00:00

abstract：:The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...

journal_title：Chromosoma

authors： Schempp W,Vogel W

更新日期：1978-06-28 00:00:00

abstract：:The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...

journal_title：Chromosoma

authors： Palmer DK,O'Day K,Margolis RL

更新日期：1990-12-01 00:00:00

abstract：:Formation of stable kinetochore-microtubule attachments is essential for accurate chromosome segregation in human cells and depends on the NDC80 complex. We recently showed that Chmp4c, an endosomal sorting complex required for transport protein involved in membrane remodelling, localises to prometaphase kinetochores ...

journal_title：Chromosoma

authors： Petsalaki E,Dandoulaki M,Zachos G

更新日期：2018-12-01 00:00:00

abstract：:Of 204 mutations located in the 8-12 band Df(2L)130 region, 37B9-C1,2;37D1-2, 199 have been assigned to twelve lethal genes and one visible gene (hook). The 13 genes are not evenly distributed. Twelve, (possibly all thirteen) are in the seven band region 37B10-C4 giving a gene-to-band ratio of almost two. Only one gen...

journal_title：Chromosoma

authors： Wright TR,Beermann W,Marsh JL,Bishop CP,Steward R,Black BC,Tomsett AD,Wright EY

更新日期：1981-01-01 00:00:00

abstract：:A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was ...

journal_title：Chromosoma

authors： Harauz G,Borland L,Bahr GF,Zeitler E,van Heel M

更新日期：1987-01-01 00:00:00

abstract：:Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observ...

journal_title：Chromosoma

authors： Cummings DJ

更新日期：1975-12-10 00:00:00

abstract：:Three new 18S.26S rRNA gene loci were identified in common wheat by sequential N-banding and in situ hybridization (ISH) analysis. Locus Nor-A7 is located at the terminal area of the long arm of 5A in both diploid and polyploid wheats. Locus Nor-B6 is located in N-band 1BL2.5 of the long arm of chromosome 1B in Tritic...

journal_title：Chromosoma

authors： Jiang J,Gill BS

更新日期：1994-06-01 00:00:00

abstract：:Short DNA regions, known to contain replication origins, were isolated from 2 M NaCl resistant nuclear structures of Physarum polycephalum after predigestion with DNase. Regions of 100 bp average length were cloned and sequenced. About 25% of the clones contained direct repeats of 12 to 16 bp and variable base sequenc...

journal_title：Chromosoma

authors： Opstelten RJ,Clement JM,Wanka F

更新日期：1989-12-01 00:00:00

abstract：:The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the fusion orientations of c...

journal_title：Chromosoma

authors： Chi JX,Huang L,Nie W,Wang J,Su B,Yang F

更新日期：2005-08-01 00:00:00