Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and antitriplex antibodies.

abstract：:Anaphase lagging of autosomes was observed in 6.1 +/- 5.4% of the primary spermatocytes in untreated larvae of the crane fly, Nephrotoma suturalis. Lagging was induced by exposure of larvae to 6 degrees C and during recovery at 22 degrees C from exposure to 0.2, 2, and 6 degrees C. The incidence of anaphase lag was ma...

journal_title：Chromosoma

authors： Janicke MA,LaFountain JR Jr

更新日期：1982-01-01 00:00:00

abstract：:In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...

journal_title：Chromosoma

authors： Narayanswami S,Doering JL,Fokta FJ,Rosenthal DS,Nguyen TN,Hamkalo BA

更新日期：1995-10-01 00:00:00

abstract：:Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...

journal_title：Chromosoma

authors： Turner BM,Keohane A

更新日期：1987-01-01 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00

abstract：:We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female c...

journal_title：Chromosoma

authors： Cowell IG,Aucott R,Mahadevaiah SK,Burgoyne PS,Huskisson N,Bongiorni S,Prantera G,Fanti L,Pimpinelli S,Wu R,Gilbert DM,Shi W,Fundele R,Morrison H,Jeppesen P,Singh PB

更新日期：2002-03-01 00:00:00

abstract：:When A-banded human chromosomes mounted in water are transferred to an organic mounting medium, the chromosomes show uniform bright quinacrine fluorescence. This change is reversible. It is inferred that quinacrine is bound uniformly along the chromosomes, and that Q-banding is a consequence of a non-uniform distribut...

journal_title：Chromosoma

authors： Sumner AT

更新日期：1977-12-06 00:00:00

abstract：:The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...

journal_title：Chromosoma

authors： Wasserzug-Pash P,Klutstein M

更新日期：2019-09-01 00:00:00

abstract：:P elements, a family of DNA transposons, are known as aggressive intruders into the hitherto uninfected gene pool of Drosophila melanogaster. Invading through horizontal transmission from an external source they managed to spread rapidly through natural populations within a few decades. Owing to their propensity for r...

journal_title：Chromosoma

authors： Pinsker W,Haring E,Hagemann S,Miller WJ

更新日期：2001-07-01 00:00:00

abstract：:An unstable minichromosome comprising part of the short arm of chromosome 10 of maize was recovered from an oil yellow variegated plant as a consequence of gamma irradiation of pollen. The cytological and gene dosage observations are consistent with the minichromosome being a partial isochromosome, which lags at mitot...

journal_title：Chromosoma

authors： Brock RD,Pryor AJ

更新日期：1996-06-01 00:00:00

abstract：:The spindle checkpoint restrains anaphase onset and mitotic exit until all chromosomes are stably attached to the mitotic spindle via their kinetochores. The Tao1 protein kinase was recently reported as a novel spindle checkpoint component. When an siRNA was used to repress Tao1, the essential spindle checkpoint compo...

journal_title：Chromosoma

authors： Westhorpe FG,Diez MA,Gurden MD,Tighe A,Taylor SS

更新日期：2010-08-01 00:00:00

abstract：:Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...

journal_title：Chromosoma

authors： Han Y,Wang G,Liu Z,Liu J,Yue W,Song R,Zhang X,Jin W

更新日期：2010-02-01 00:00:00

abstract：:The irradiation of metaphase spreads of human cells with ultraviolet (UV) light blocked the chromosome banding induced by Alu I, Mbo I, Dde I, Hinf I, Hae III, and Rsa I restriction endonucleases. At 13 J/m2 there was moderate inhibition of the nuclease action, which was detected as an increase in the stain intensity ...

journal_title：Chromosoma

authors： Bianchi NO,Bianchi MS,Cleaver JE

更新日期：1984-01-01 00:00:00

abstract：:Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...

journal_title：Chromosoma

authors： Cahoon CK,Libuda DE

更新日期：2019-09-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...

journal_title：Chromosoma

authors： Capozzi O,Archidiacono N,Lorusso N,Stanyon R,Rocchi M

更新日期：2016-09-01 00:00:00

abstract：:Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...

journal_title：Chromosoma

authors： Howell WM,Hsu TC

更新日期：1979-06-21 00:00:00

abstract：:Coilin, a molecular marker for Cajal bodies (CBs), is a phosphoprotein that contains a cryptic nucleolar localization signal and multiple interacting domains, such as the RG-box. Post-translational symmetrical dimethylation of arginines on the coilin RG-box is required for the recruitment of the survival motor neuron ...

journal_title：Chromosoma

authors： Tapia O,Bengoechea R,Berciano MT,Lafarga M

更新日期：2010-10-01 00:00:00

abstract：:The large heterochromatic chromosome of Drosophila nasutoides reveals distinctive C- and Q-bands. The regions which are negative in C-banding appear positive in Q-banding. The isochromosomic nature of this chromosome and the locality of the satellite DNAs in this chromosome are discussed with respect to these banding ...

journal_title：Chromosoma

authors： Lee CS,Collins L

更新日期：1977-04-27 00:00:00

abstract：:Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focus...

journal_title：Chromosoma

authors： Solinhac R,Mompart F,Martin P,Robelin D,Pinton P,Iannuccelli E,Lahbib-Mansais Y,Oswald IP,Yerle-Bouissou M

更新日期：2011-10-01 00:00:00

abstract：:Nucleolar matrix structures were obtained under different extraction conditions from highly purified isolated nucleoli. Their ultrastructural appearance, protein composition and capacity to bind rDNA preferentially were studied in a model binding system. A region spanning approximately 25 kb in the rat ribosomal gene ...

journal_title：Chromosoma

authors： Stephanova E,Stancheva R,Avramova Z

更新日期：1993-03-01 00:00:00

abstract：:In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...

journal_title：Chromosoma

authors： Van Hooser AA,Yuh P,Heald R

更新日期：2005-12-01 00:00:00

abstract：:In wild-type Drosophila hydei (genotype X/Y) four different primary spermatocyte nuclear glycoproteins, classified as non-Y encoded because of their occurrence in X/O genotypes, were demonstrated to possess a few epitopes that depended on formation of the Y chromosomal giant lampbrush loops threads (th; Mr 55,000 prot...

journal_title：Chromosoma

authors： Tischendorf G,Liebrich W,Trapitz P,Wood G,Schwochau M

更新日期：1989-08-01 00:00:00

abstract：:Macronuclear DNA s from three related hypotrichous ciliated protozoans were compared by agarose gel electrophoresis. Each was shown to be composed of DNA duplexes that yielded a unique pattern of bands overlying a continuous distribution of DNA sizes ranging from approximately 400 bp to approximately 20,000 bp. By EM,...

journal_title：Chromosoma

authors： Swanton MT,Heumann JM,Prescott DM

更新日期：1980-01-01 00:00:00

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:Normal lepidopteran males produce two kinds of spermatozoa: nucleate (eupyrene) and anucleate (apyrene). Eupyrene spermatozoa have the usual type of elongate nuclei. But in apyrene spermatids, the nuclei never elongate and the chromatin remains in a telophase-like condition until enucleation occurs. The study of the d...

journal_title：Chromosoma

authors： Friedländer M,Hauschteck-Jungen E

更新日期：1982-01-01 00:00:00

abstract：:The role of helix 6, which forms the major portion of the most 5'-located expansion segment of Saccharomyces cerevisiae 18S rRNA, was studied by in vivo mutational analysis. Mutations that increased the size of the helical part and/or the loop, even to a relatively small extent, abolished 18S rRNA formation almost com...

journal_title：Chromosoma

authors： van Nues RW,Venema J,Planta RJ,Raué HA

更新日期：1997-06-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

journal_title：Chromosoma

authors： Coelho PA,Nurminsky D,Hartl D,Sunkel CE

更新日期：1996-10-01 00:00:00

abstract：:Facioscapulohumeral muscular dystrophy (FSHD) is caused by the shortening of a copy-number polymorphic array of 3.3 kb repeats (D4Z4) at one allelic 4q35.2 region. How this contraction of a subtelomeric tandem array causes FSHD is unknown but indirect evidence suggests that a short array has a cis effect on a distant ...

journal_title：Chromosoma

authors： Yang F,Shao C,Vedanarayanan V,Ehrlich M

更新日期：2004-05-01 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:PICH (Plk1-interacting checkpoint helicase) was recently identified as an essential component of the spindle assembly checkpoint and shown to localize to kinetochores, inner centromeres, and thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in si...

journal_title：Chromosoma

authors： Wang LH,Schwarzbraun T,Speicher MR,Nigg EA

更新日期：2008-04-01 00:00:00

abstract：:In the domestic pig (2n = 38) two types of constitutive heterochromatin can be differentiated by fluorescence counterstaining techniques. All 24 biarmed autosomes and the X chromosome have chromomycin A3-positive centromeric C-bands, whereas all 12 acrocentric chromosomes exhibit DA-DAPI-positive centromeric heterochr...

journal_title：Chromosoma

authors： Schwarzacher T,Mayr B,Schweizer D

更新日期：1984-01-01 00:00:00