Molecular cloning of zebra finch W chromosome repetitive sequences: evolution of the avian W chromosome.

abstract：:Polo-like kinase 1 (Plk1) and Aurora A play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Here we show that the functions of these kinases during early mitosis are coordinated through Bora, a partner of Aurora A first identified in Drosophila. Depletion of human...

journal_title：Chromosoma

authors： Chan EH,Santamaria A,Silljé HH,Nigg EA

更新日期：2008-10-01 00:00:00

abstract：:The kinetochore binds an evolutionarily conserved set of checkpoint proteins that function to monitor whether chromosomes have aligned properly at the spindle equator. Human cells contain two related protein kinases, hBUB1 and hBUBR1, that appear to have evolved from a single ancestral BUB1 gene. We generated hBUB1- a...

journal_title：Chromosoma

authors： Jablonski SA,Chan GK,Cooke CA,Earnshaw WC,Yen TJ

更新日期：1998-12-01 00:00:00

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:Chromosome behaviour during meiosis in male Syrian hamsters heterozygous for one of three translocations was analysed as part of a study of the transmission of these structural changes. Synapsis was studied using preparations of synaptonemal complexes, and chiasmate associations and the results of anaphase I segregati...

journal_title：Chromosoma

authors： Cawood AH,Breckon G

更新日期：1989-10-01 00:00:00

abstract：:Aurora-A is a conserved mitotic kinase overexpressed in many types of cancer. Growing evidence shows that Aurora-A plays a crucial role in DNA damage response (DDR) although this aspect has been less characterized. We isolated a new aur-A mutation, named aur-A949, in Drosophila, and we showed that it causes chromosome...

journal_title：Chromosoma

authors： Merigliano C,Mascolo E,Cesta A,Saggio I,Vernì F

更新日期：2019-03-01 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00

abstract：:The number of RNA molecules per cell (transcriptome size) is highly variable, differing among and within cell types depending on cell size, stage of the cell cycle, ploidy level, age, disease state, and growth condition. Such variation has been observed at the level of total RNA, ribosomal RNA, messenger RNA (mRNA), a...

journal_title：Chromosoma

authors： Coate JE,Doyle JJ

更新日期：2015-03-01 00:00:00

abstract：:The two-hybrid system was used to identify proteins that interact with the central conserved domain of Saccharomyces cerevisiae DNA topoisomerase I. Several different C-terminal domain-containing fragments of topoisomerase I, none of which overlapped with the central domain, were identified as specific interacting pol...

journal_title：Chromosoma

authors： Park H,Sternglanz R

更新日期：1998-06-01 00:00:00

abstract：:Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-...

journal_title：Chromosoma

authors： Zakrzewski F,Weisshaar B,Fuchs J,Bannack E,Minoche AE,Dohm JC,Himmelbauer H,Schmidt T

更新日期：2011-08-01 00:00:00

abstract：:In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this techniqu...

journal_title：Chromosoma

authors： Liu JY,She CW,Hu ZL,Xiong ZY,Liu LH,Song YC

更新日期：2004-08-01 00:00:00

abstract：:The organization of DNA in the mitotic metaphase and polytene chromosomes of the fungus gnat, Sciara coprophila, has been studied using base-specific DNA ligands, including anti-nucleoside antibodies. The DNA of metaphase and polytene chromosomes reacts with AT-specific probes (quinacrine, DAPI, Hoechst 33258 and anti...

journal_title：Chromosoma

authors： Eastman EM,Goodman RM,Erlanger BF,Miller OJ

更新日期：1980-01-01 00:00:00

abstract：:Insulator proteins orchestrate the three-dimensional organization of the genome. Insulators function by facilitating communications between regulatory sequences and gene promoters, allowing accurate gene transcription regulation during embryo development and cell differentiation. However, the role of insulator protein...

journal_title：Chromosoma

authors： Hsu SJ,Stow EC,Simmons JR,Wallace HA,Lopez AM,Stroud S,Labrador M

更新日期：2020-12-01 00:00:00

abstract：:Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused ...

journal_title：Chromosoma

authors： Ansari HA,Maher DW,Pearce PD,Broad TE

更新日期：1996-07-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:We describe a mammalian artificial mini-chromosome lacking human alphoid DNA and mouse minor and major satellite DNA repeats. This mini-chromosome, initially recovered in a mouse embryonic stem (ES) cell line (CGR8), is 2.6 Mb in size and consists of sequences derived from the human Y chromosome and mouse chromosomes ...

journal_title：Chromosoma

authors： Shen MH,Ross A,Yang J,de las Heras JI,Cooke H

更新日期：2001-11-01 00:00:00

abstract：:The distinctive behaviors of chromosomes in mitosis and meiosis depend upon differences in kinetochore position. Kinetochore position is well established except for a critical transition between meiosis I and meiosis II. We examined kinetochore position during the transition and compared it with the position of kineto...

journal_title：Chromosoma

authors： Paliulis LV,Nicklas RB

更新日期：2005-03-01 00:00:00

abstract：:Histone modifications regulate key processes of eukaryotic genomes. Misregulation of the enzymes that place these modifications can lead to disease. An example of this is DOT1L, the enzyme that can mono-, di-, and trimethylate the nucleosome core on lysine 79 of histone H3 (H3K79). DOT1L plays a role in development an...

journal_title：Chromosoma

authors： Vlaming H,van Leeuwen F

更新日期：2016-09-01 00:00:00

abstract：:The hsp70, hsp83, hsromega, and the small heat shock protein genes were mapped on the polytene chromosomes of six species, representative of the geographical distribution of the Drosophila montium subgroup of the melanogaster species group. In addition, based on hybridization conditions, the putative locus of the hsp6...

journal_title：Chromosoma

authors： Drosopoulou E,Konstantopoulou I,Scouras ZG

更新日期：1996-08-01 00:00:00

abstract：:P elements, a family of DNA transposons, are known as aggressive intruders into the hitherto uninfected gene pool of Drosophila melanogaster. Invading through horizontal transmission from an external source they managed to spread rapidly through natural populations within a few decades. Owing to their propensity for r...

journal_title：Chromosoma

authors： Pinsker W,Haring E,Hagemann S,Miller WJ

更新日期：2001-07-01 00:00:00

abstract：:It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

journal_title：Chromosoma

authors： Elgin SC,Boyd JB

更新日期：1975-01-01 00:00:00

abstract：:In wild-type Drosophila hydei (genotype X/Y) four different primary spermatocyte nuclear glycoproteins, classified as non-Y encoded because of their occurrence in X/O genotypes, were demonstrated to possess a few epitopes that depended on formation of the Y chromosomal giant lampbrush loops threads (th; Mr 55,000 prot...

journal_title：Chromosoma

authors： Tischendorf G,Liebrich W,Trapitz P,Wood G,Schwochau M

更新日期：1989-08-01 00:00:00

abstract：:Studies performed on human trisomic 21 oocytes have revealed that during meiosis, the three homologues 21 synapse and, in some cases, achieve what looks like a trivalent. This implies that meiotic recombination takes place among the three homologous chromosomes 21, and to some extent, crossovers form between them. To ...

journal_title：Chromosoma

authors： Robles P,Roig I,Garcia R,Brieño-Enríquez M,Martin M,Cabero L,Toran N,Garcia Caldés M

更新日期：2013-03-01 00:00:00

abstract：:The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres vari...

journal_title：Chromosoma

authors： Lermontova I,Rutten T,Schubert I

更新日期：2011-12-01 00:00:00

abstract：:The cotyledon parenchyma cells of common bean (Phaseolus vulgaris L.) produce large quantities of storage proteins during embryo maturation; throughout this period, these cells also accumulate nuclear DNA (nDNA). To investigate the basis of this nDNA accumulation, we have measured storage protein mRNA pools, nDNA mass...

journal_title：Chromosoma

authors： Johnson KA,Sussex IM

更新日期：1990-07-01 00:00:00

abstract：:Macronuclear DNA of four hypotrichous and one holotrichous ciliate species was characterized by biochemical techniques. The renaturation kinetics of the macronuclear DNAs of all five species were similar. Repetitive sequences occur only in an amount below 2%. Although the DNA content of the macronuclei of the species ...

journal_title：Chromosoma

authors： Steinbrück G,Haas I,Hellmer KH,Ammermann D

更新日期：1981-01-01 00:00:00

abstract：:In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...

journal_title：Chromosoma

authors： Qiao H,Offenberg HH,Anderson LK

更新日期：2012-06-01 00:00:00

abstract：:The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...

journal_title：Chromosoma

authors： Hendzel MJ,Bazett-Jones DP

更新日期：1995-02-01 00:00:00

abstract：:We probed the complex between oriC and DnaA protein using two types of mutants in oriC. Base changes in the DnaA binding sites, DnaA boxes, had little effect on origin function. Mutations which change the distance between DnaA boxes R3 and R4, on the other hand, inactivated oriC unless the mutation deleted or inserted...

journal_title：Chromosoma

authors： Messer W,Hartmann-Kühlein H,Langer U,Mahlow E,Roth A,Schaper S,Urmoneit B,Woelker B

更新日期：1992-01-01 00:00:00

abstract：:The dioecious plant Silene latifolia has large, heteromorphic X and Y sex chromosomes that are thought to be derived from rearrangements of autosomes. To reveal the origin of the sex chromosomes in S. latifolia, we isolated and characterized telomere-homologous sequences from intra-chromosomal regions (interstitial te...

journal_title：Chromosoma

authors： Uchida W,Matsunaga S,Sugiyama R,Shibata F,Kazama Y,Miyazawa Y,Hizume M,Kawano S

更新日期：2002-12-01 00:00:00

abstract：:Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...

journal_title：Chromosoma

authors： Bähler J,Hagens G,Holzinger G,Scherthan H,Heyer WD

更新日期：1994-04-01 00:00:00