Abstract:
:Insulator proteins orchestrate the three-dimensional organization of the genome. Insulators function by facilitating communications between regulatory sequences and gene promoters, allowing accurate gene transcription regulation during embryo development and cell differentiation. However, the role of insulator proteins beyond genome organization and transcription regulation remains unclear. Suppressor of Hairy wing [Su(Hw)] is a Drosophila insulator protein that plays an important function in female oogenesis. Here we find that su(Hw) has an unsuspected role in genome stability during cell differentiation. We show that su(Hw) mutant developing egg chambers have poorly formed microtubule organization centers (MTOCs) in the germarium and display mislocalization of the anterior/posterior axis specification factor gurken in later oogenesis stages. Additionally, eggshells from partially rescued su(Hw) mutant female germline exhibit dorsoventral patterning defects. These phenotypes are very similar to phenotypes found in the important class of spindle mutants or in piRNA pathway mutants in Drosophila, in which defects generally result from the failure of germ cells to repair DNA damage. Similarities between mutations in su(Hw) and spindle and piRNA mutants are further supported by an excess of DNA damage in nurse cells, and because Gurken localization defects are partially rescued by mutations in the ATR (mei-41) and Chk1 (grapes) DNA damage response genes. Finally, we also show that su(Hw) mutants produce an elevated number of chromosome breaks in dividing neuroblasts from larval brains. Together, these findings suggest that Su(Hw) is necessary for the maintenance of genome integrity during Drosophila development, in both germline and dividing somatic cells.
journal_name
Chromosomajournal_title
Chromosomaauthors
Hsu SJ,Stow EC,Simmons JR,Wallace HA,Lopez AM,Stroud S,Labrador Mdoi
10.1007/s00412-020-00743-8subject
Has Abstractpub_date
2020-12-01 00:00:00pages
255-274issue
3-4eissn
0009-5915issn
1432-0886pii
10.1007/s00412-020-00743-8journal_volume
129pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288461
更新日期:1977-03-16 00:00:00
abstract::The MRE11/RAD50/NBS1 complex (Mre11 complex) is a central player in most aspects of the cellular response to DNA double-strand breaks, including homologous recombination, non-homologous end joining, telomere maintenance and DNA damage checkpoint activation. Several of these findings are explained by the unusual enzyma...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-004-0306-4
更新日期:2004-10-01 00:00:00
abstract::In Drosophila as in many organisms beta tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the beta 1 and beta 2 tubulins of Drosophila melanogaster and the beta 2 tubulin of D. hydei, and found these insect beta tubulins to be highly conserved and like beta tubulins...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00333989
更新日期:1987-01-01 00:00:00
abstract::The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120100148
更新日期:2001-08-01 00:00:00
abstract::Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-009-0239-z
更新日期:2010-02-01 00:00:00
abstract::Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050343
更新日期:1998-12-01 00:00:00
abstract::Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded state...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0445-6
更新日期:2014-06-01 00:00:00
abstract::The Balbiani rings in the salivary gland polytene chromosomes of Chironomus tentans include the most active structural genes in this organ. Two of them (BR1 and BR2) contain repetitive sequences and are transcribed into giant RNA molecules. On Southern blots of restriction digests, we have identified fragments of geno...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327354
更新日期:1981-01-01 00:00:00
abstract::The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328768
更新日期:1979-01-31 00:00:00
abstract::cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294968
更新日期:1982-01-01 00:00:00
abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293338
更新日期:1989-06-01 00:00:00
abstract::Diverse studies indicate that certain nonhistone proteins may be responsible for maintaining the integrity of the eukaryotic chromosomes. In view of their dispersal during nuclear division and their acknowledged role in gene expression, none of the nuclear matrix proteins can be regarded as integral proteins of the ch...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-002-0189-1
更新日期:2002-07-01 00:00:00
abstract::Male hybrids of the cross D. azteca X D. athabasca are larger (hybrid giant males) than their parents, whereas hybrid females are of the same size as the parental species. Microspectrophotometric measurements have shown that the larval polytene salivary gland chromosomes of hybrid giant males undergo one more endorepl...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00295210
更新日期:1976-09-10 00:00:00
abstract::Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00291004
更新日期:1985-01-01 00:00:00
abstract::Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286790
更新日期:1981-01-01 00:00:00
abstract::Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292229
更新日期:1980-01-01 00:00:00
abstract::The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293114
更新日期:1976-06-30 00:00:00
abstract::Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00331572
更新日期:1979-08-10 00:00:00
abstract::The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-014-0479-4
更新日期:2014-12-01 00:00:00
abstract::The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327596
更新日期:1986-01-01 00:00:00
abstract::Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of cent...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00357684
更新日期:1995-05-01 00:00:00
abstract::In budding yeast, mitotic DNA replication initiates at sequence-specific replication origins, the prototype for which is ARS1. Initiation serves as the primary control point for mitotic DNA replication, and is catalyzed by the Cdc7 protein kinase. In contrast, premeiotic DNA replication apparently does not require Cdc...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00360406
更新日期:1993-06-01 00:00:00
abstract::The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-016-0604-7
更新日期:2017-02-01 00:00:00
abstract::Nucleolar matrix structures were obtained under different extraction conditions from highly purified isolated nucleoli. Their ultrastructural appearance, protein composition and capacity to bind rDNA preferentially were studied in a model binding system. A region spanning approximately 25 kb in the rat ribosomal gene ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352403
更新日期:1993-03-01 00:00:00
abstract::Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330781
更新日期:1982-01-01 00:00:00
abstract::In the parasitic wasp Nasonia vitripennis, certain males carry a B chromosome, called PSR (paternal sex ratio), which causes the compaction and subsequent loss of the paternal chromosomes in fertilized eggs. Because Nasonia are haplo-diploid, this leads to the production of all-male broods. Three families (PSR2, PSR18...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00660317
更新日期:1992-08-01 00:00:00
abstract::The zebra finch (Taeniopygia guttata) has a large Z chromosome and highly condensed W chromosome. We used the random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique to isolate female-specific sequences ZBM1 and ZBM2. Southern blot hybridization to male and female zebra finch genomic DNA sugg...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-007-0130-8
更新日期:2008-04-01 00:00:00
abstract::The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352253
更新日期:1994-07-01 00:00:00
abstract::A system has been developed using a microscope photometer to measure the DNA content of individual chromosomes. After correcting for the systematic and statistical errors the measured values are highly reproducible. Data are presented of measurements of human Y chromosomes from a cytogenetically normal population. Whe...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00371969
更新日期:1989-01-01 00:00:00
abstract::We characterized four meiotic mutants of the fission yeast Schizosaccharomyces pombe by live observation of nuclear movement. Nuclei were stained with either the DNA-specific fluorescent dye Hoechst 33342 or jellyfish green fluorescent protein (GFP) fused with the N-terminal portion of DNA polymerase alpha. We first f...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050417
更新日期:2000-01-01 00:00:00