Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement.

abstract：:A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous...

journal_title：Chromosoma

authors： Scalenghe F,Buscaglia M,Steinheil C,Crippa M

更新日期：1978-05-16 00:00:00

abstract：:A method is described for excising by a glass knife single bands of isolated polytene chromosomes of the salivary glands of Chironomus tentans larvae. DNA strands were isolated from cut-out bands and their contour lengths were determined on electron micrographs. The mean contour length of DNA strands isolated from the...

journal_title：Chromosoma

authors： Frey M,Koller T,Lezzi M

更新日期：1982-01-01 00:00:00

abstract：:P elements, a family of DNA transposons, are known as aggressive intruders into the hitherto uninfected gene pool of Drosophila melanogaster. Invading through horizontal transmission from an external source they managed to spread rapidly through natural populations within a few decades. Owing to their propensity for r...

journal_title：Chromosoma

authors： Pinsker W,Haring E,Hagemann S,Miller WJ

更新日期：2001-07-01 00:00:00

abstract：:The chromatin protein Polycomb (PC) is necessary for keeping homeotic genes repressed in a permanent and heritable manner. PC is part of a large multimeric complex (PcG proteins) involved in generating silenced chromatin domains at target genes, thus preventing their inappropriate expression. In order to assess the in...

journal_title：Chromosoma

authors： Dietzel S,Niemann H,Brückner B,Maurange C,Paro R

更新日期：1999-05-01 00:00:00

abstract：:Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...

journal_title：Chromosoma

authors： Mancino G,Ragghianti M,Bucci-Innocenti S

更新日期：1979-08-10 00:00:00

abstract：:The distribution of intermicrotubular bridges in spindles of tipulid spermatocytes (Pales ferruginea, first meiotic division) was analyzed using serial sections of pre-selected cells. Bridges were found in all spindle regions, including kinetochore microtubules and free microtubules in the chromosome fiber. The dimens...

journal_title：Chromosoma

authors： Bastmeyer M,Fuge H

更新日期：1986-01-01 00:00:00

abstract：:Male hybrids of the cross D. azteca X D. athabasca are larger (hybrid giant males) than their parents, whereas hybrid females are of the same size as the parental species. Microspectrophotometric measurements have shown that the larval polytene salivary gland chromosomes of hybrid giant males undergo one more endorepl...

journal_title：Chromosoma

authors： Meer B

更新日期：1976-09-10 00:00:00

abstract：:Telomeric DNA is present at the ends of eukaryotic chromosomes and is bound by telomere "capping" proteins, which are the (Cdc13-Stn1-Ten1) CST complex, Ku (Yku70-Yku80), and Rap1-Rif1-Rif2 in budding yeast. Inactivation of any of these complexes causes telomere "uncapping," stimulating a DNA damage response (DDR) tha...

journal_title：Chromosoma

authors： Dewar JM,Lydall D

更新日期：2012-04-01 00:00:00

abstract：:Facioscapulohumeral muscular dystrophy (FSHD) is caused by the shortening of a copy-number polymorphic array of 3.3 kb repeats (D4Z4) at one allelic 4q35.2 region. How this contraction of a subtelomeric tandem array causes FSHD is unknown but indirect evidence suggests that a short array has a cis effect on a distant ...

journal_title：Chromosoma

authors： Yang F,Shao C,Vedanarayanan V,Ehrlich M

更新日期：2004-05-01 00:00:00

abstract：:Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...

journal_title：Chromosoma

authors： Eichenlaub-Ritter U,Ruthmann A

更新日期：1982-01-01 00:00:00

abstract：:We describe a mammalian artificial mini-chromosome lacking human alphoid DNA and mouse minor and major satellite DNA repeats. This mini-chromosome, initially recovered in a mouse embryonic stem (ES) cell line (CGR8), is 2.6 Mb in size and consists of sequences derived from the human Y chromosome and mouse chromosomes ...

journal_title：Chromosoma

authors： Shen MH,Ross A,Yang J,de las Heras JI,Cooke H

更新日期：2001-11-01 00:00:00

abstract：:We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

journal_title：Chromosoma

authors： Lifschytz E,Hareven D

更新日期：1982-01-01 00:00:00

abstract：:After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repe...

journal_title：Chromosoma

authors： Houben A,Wanner G,Hanson L,Verlin D,Leach CR,Timmis JN

更新日期：2000-06-01 00:00:00

abstract：:By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...

journal_title：Chromosoma

authors： Livolant F

更新日期：1978-08-21 00:00:00

abstract：:From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...

journal_title：Chromosoma

authors： Roth GE

更新日期：1991-05-01 00:00:00

abstract：:Genomic sequencing reveals similar but limited numbers of protein-coding genes in different genomes, which begs the question of how organismal diversities are generated. Alternative pre-mRNA splicing, a widespread phenomenon in higher eukaryotic genomes, is thought to provide a mechanism to increase the complexity of ...

journal_title：Chromosoma

authors： Zhou Z,Fu XD

更新日期：2013-06-01 00:00:00

abstract：:The influence of the sex-reversal factor (Sxr) on X and Y chromosome pairing was examined by comparing males with "novel" and "standard" Sxr genotypes. The novel Sxr males were exceptional in carrying Sxr on their X rather than their Y chromosome, or homozygously on both their X and Y chromosomes, or on a Y chromosome...

journal_title：Chromosoma

authors： Tease C,Cattanach BM

更新日期：1989-03-01 00:00:00

abstract：:Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...

journal_title：Chromosoma

authors： Dowsett AP

更新日期：1983-01-01 00:00:00

abstract：:The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...

journal_title：Chromosoma

authors： Ohno M,Fukagawa T,Lee JS,Ikemura T

更新日期：2002-09-01 00:00:00

abstract：:Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication pa...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1992-03-01 00:00:00

abstract：:With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...

journal_title：Chromosoma

authors： Funaki K,Matsui S,Sasaki M

更新日期：1975-01-01 00:00:00

abstract：:The synaptonemal complexes of surface-spread spermatocytes of mice heterozygous for one of two reciprocal translations (R3 and R5) between the X and chromosome 7 have been examined by light and electron microscopy (EM). The break points of R3 were determined to be at 70% of chromosome 7, as measured from the centromer...

journal_title：Chromosoma

authors： Ashley T,Russell LB,Cacheiro NL

更新日期：1982-01-01 00:00:00

abstract：:The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...

journal_title：Chromosoma

authors： Scherthan H,Schöfisch K,Dell T,Illner D

更新日期：2014-12-01 00:00:00

abstract：:Formation of stable kinetochore-microtubule attachments is essential for accurate chromosome segregation in human cells and depends on the NDC80 complex. We recently showed that Chmp4c, an endosomal sorting complex required for transport protein involved in membrane remodelling, localises to prometaphase kinetochores ...

journal_title：Chromosoma

authors： Petsalaki E,Dandoulaki M,Zachos G

更新日期：2018-12-01 00:00:00

abstract：:Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...

journal_title：Chromosoma

authors： Greenleaf AL,Plagens U,Jamrich M,Bautz EK

更新日期：1978-01-16 00:00:00

abstract：:Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

journal_title：Chromosoma

authors： Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

更新日期：2012-10-01 00:00:00

abstract：:It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

journal_title：Chromosoma

authors： Elgin SC,Boyd JB

更新日期：1975-01-01 00:00:00

abstract：:At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We repo...

journal_title：Chromosoma

authors： Moens PB,Earnshaw WC

更新日期：1989-11-01 00:00:00

abstract：:Studies using human and mouse cells have revealed some changes to non-telomeric chromatin and gene expression in response to abnormally short telomeres. To investigate this further, we studied the effect of inheriting shorter telomeres on transcription and genetic stability at non-telomeric sites in the mouse. Using m...

journal_title：Chromosoma

authors： Roberts AR,Huang E,Jones L,Daxinger L,Chong S,Whitelaw E

更新日期：2013-12-01 00:00:00

abstract：:cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...

journal_title：Chromosoma

authors： Peters FP,Grond CJ,Sondermeijer PJ,Lubsen NH

更新日期：1982-01-01 00:00:00