Demonstration of replication patterns in the last premeiotic S-phase of male Chinese hamsters after BrdU pulse labeling.

Abstract:

:Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication patterns observed are similar to that of somatic cells. (2) The heterochromatin starts replication in early S-phase. (3) The euchromatic part of the X chromosome of the male Chinese hamster replicates together with the autosomes and therefore behaves isocyclicly and not allocyclicly as hitherto assumed. Hence, genetic inactivity of the X chromosome may be brought about by a mechanism different from that in somatic cells.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Latos-Bielenska A,Vogel W

doi

10.1007/BF00346006

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

279-83

issue

5-6

eissn

0009-5915

issn

1432-0886

journal_volume

101

pub_type

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