Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.

abstract：:The molecular structure of chromatin during dogfish spermiogenesis was examined by electron microscopy after the dispersion of nuclei at low ionic strength. In early and late stages of differentiation (round and elongating spermatids), chromatin is globular, although basic nuclear proteins are different from those pre...

journal_title：Chromosoma

authors： Gusse M,Chevaillier P

更新日期：1980-01-01 00:00:00

abstract：:Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...

journal_title：Chromosoma

authors： Dowsett AP

更新日期：1983-01-01 00:00:00

abstract：:A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability....

journal_title：Chromosoma

authors： Tognetti S,Riera A,Speck C

更新日期：2015-03-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...

journal_title：Chromosoma

authors： Capozzi O,Archidiacono N,Lorusso N,Stanyon R,Rocchi M

更新日期：2016-09-01 00:00:00

abstract：:The large heterochromatic chromosome of Drosophila nasutoides reveals distinctive C- and Q-bands. The regions which are negative in C-banding appear positive in Q-banding. The isochromosomic nature of this chromosome and the locality of the satellite DNAs in this chromosome are discussed with respect to these banding ...

journal_title：Chromosoma

authors： Lee CS,Collins L

更新日期：1977-04-27 00:00:00

abstract：:The Balbiani rings in the salivary gland polytene chromosomes of Chironomus tentans include the most active structural genes in this organ. Two of them (BR1 and BR2) contain repetitive sequences and are transcribed into giant RNA molecules. On Southern blots of restriction digests, we have identified fragments of geno...

journal_title：Chromosoma

authors： Degelmann A,Hollenberg CP

更新日期：1981-01-01 00:00:00

abstract：:A cytological procedure for in vitro chromosome delineation has been studied using human and mouse (Mus musculus) chromosomes. This method, consisting of slide incubation in KMnO4 at 0--5 degrees C for 24 h followed by a short exposure to NaHSO3 (1--3 min) and Giemsa staining, induces extraction of chromatin from huma...

journal_title：Chromosoma

authors： Drets ME,Novello A

更新日期：1980-01-01 00:00:00

abstract：:Compelling evidence has been obtained in favour of the idea that the nuclear surface of higher plant cells is a microtubule-nucleating and/or organizing site (MTOC), in the absence of defined centrosomes. How these plant MTOC proteins are redistributed and function during the progression of the cell cycle remains enti...

journal_title：Chromosoma

authors： Schmit AC,Stoppin V,Chevrier V,Job D,Lambert AM

更新日期：1994-09-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00

abstract：:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...

journal_title：Chromosoma

authors： Schäfer R,Ali S,Epplen JT

更新日期：1986-01-01 00:00:00

abstract：:We characterized four meiotic mutants of the fission yeast Schizosaccharomyces pombe by live observation of nuclear movement. Nuclei were stained with either the DNA-specific fluorescent dye Hoechst 33342 or jellyfish green fluorescent protein (GFP) fused with the N-terminal portion of DNA polymerase alpha. We first f...

journal_title：Chromosoma

authors： Hiraoka Y,Ding DQ,Yamamoto A,Tsutsumi C,Chikashige Y

更新日期：2000-01-01 00:00:00

abstract：:A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was ...

journal_title：Chromosoma

authors： Harauz G,Borland L,Bahr GF,Zeitler E,van Heel M

更新日期：1987-01-01 00:00:00

abstract：:Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...

journal_title：Chromosoma

authors： Greenleaf AL,Plagens U,Jamrich M,Bautz EK

更新日期：1978-01-16 00:00:00

abstract：:Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of cent...

journal_title：Chromosoma

authors： Zalensky AO,Allen MJ,Kobayashi A,Zalenskaya IA,Balhórn R,Bradbury EM

更新日期：1995-05-01 00:00:00

abstract：:Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...

journal_title：Chromosoma

authors： Turner BM,Keohane A

更新日期：1987-01-01 00:00:00

abstract：:Chromosome segregation ensures the equal partitioning of chromosomes at mitosis. However, long chromosome arms may pose a problem for complete sister chromatid separation. In this paper we report on the analysis of cell division in primary cells from field vole Microtus agrestis, a species with 52 chromosomes includin...

journal_title：Chromosoma

authors： Rens W,Torosantucci L,Degrassi F,Ferguson-Smith MA

更新日期：2006-12-01 00:00:00

abstract：:The distribution of intermicrotubular bridges in spindles of tipulid spermatocytes (Pales ferruginea, first meiotic division) was analyzed using serial sections of pre-selected cells. Bridges were found in all spindle regions, including kinetochore microtubules and free microtubules in the chromosome fiber. The dimens...

journal_title：Chromosoma

authors： Bastmeyer M,Fuge H

更新日期：1986-01-01 00:00:00

abstract：:Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...

journal_title：Chromosoma

authors： Mancino G,Ragghianti M,Bucci-Innocenti S

更新日期：1979-08-10 00:00:00

abstract：:By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...

journal_title：Chromosoma

authors： Kato H

更新日期：1977-02-03 00:00:00

abstract：:From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...

journal_title：Chromosoma

authors： Roth GE

更新日期：1991-05-01 00:00:00

abstract：:The DNA of eukaryotic cells is organized in a complex with proteins, either as interphase chromatin or mitotic chromosomes. Nucleosomes, the structural subunits of chromatin, have long been considered as static structures, incompatible with processes occurring in chromatin. During the past few years it has become evid...

journal_title：Chromosoma

authors： Loidl P

更新日期：1994-12-01 00:00:00

abstract：:A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...

journal_title：Chromosoma

authors： Satya-Prakash KL,Hsu TC,Pathak S

更新日期：1980-01-01 00:00:00

abstract：:The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...

journal_title：Chromosoma

authors： Schempp W,Vogel W

更新日期：1978-06-28 00:00:00

abstract：:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...

journal_title：Chromosoma

authors： Melnikova L,Kostyuchenko M,Molodina V,Parshikov A,Georgiev P,Golovnin A

更新日期：2018-03-01 00:00:00

abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...

journal_title：Chromosoma

authors： Higgins JM

更新日期：2010-04-01 00:00:00

abstract：:Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...

journal_title：Chromosoma

authors： Han Y,Wang G,Liu Z,Liu J,Yue W,Song R,Zhang X,Jin W

更新日期：2010-02-01 00:00:00

abstract：:Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...

journal_title：Chromosoma

authors： Taruscio D,Manuelidis L

更新日期：1991-12-01 00:00:00

abstract：:This investigation was conducted to determine the relationship between Y chromosome loss and increased micronucleus formation with age. We also investigated the status of kinetochore proteins in the micronuclei. Umbilical cord blood samples were obtained from 18 newborn males, and peripheral blood was obtained from 35...

journal_title：Chromosoma

authors： Nath J,Tucker JD,Hando JC

更新日期：1995-07-01 00:00:00

abstract：:Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces ...

journal_title：Chromosoma

authors： Foulk MS,Waggener JM,Johnson JM,Yamamoto Y,Liew GM,Urnov FD,Young Y,Lee G,Smith HS,Gerbi SA

更新日期：2013-03-01 00:00:00

abstract：:At least two types of Robertsonian exchange are now known in the acrocentric chromosomes of man. Both types involve breakage in the arms adjacent to the centromere. Evidence is presented for a third type of exchange, one involving breakage within the centromere itself, in the grasshopper Percassa rugifrons. In this sp...

journal_title：Chromosoma

authors： John B,Freeman M

更新日期：1975-09-26 00:00:00