Interactions between BTB domain of CP190 and two adjacent regions in Su(Hw) are required for the insulator complex formation.

Abstract:

:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many other architectural proteins. In spite of the fact that CP190 is critical for the activity of Su(Hw) insulators, interaction between these proteins has not been studied yet. Therefore, we have performed a detailed analysis of domains involved in the interaction between the Su(Hw) and CP190. The results show that the BTB domain of CP190 interacts with two adjacent regions at the N-terminus of Su(Hw). Deletion of either region in Su(Hw) only weakly affected recruiting of CP190 to the Su(Hw) sites in the presence of Mod(mdg4)-67.2. Deletion of both regions in Su(Hw) prevents its interaction with CP190. Using mutations in vivo, we found that interactions with Su(Hw) and Mod(mdg4)-67.2 are essential for recruiting of CP190 to the Su(Hw) genomic sites.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Melnikova L,Kostyuchenko M,Molodina V,Parshikov A,Georgiev P,Golovnin A

doi

10.1007/s00412-017-0645-6

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

59-71

issue

1

eissn

0009-5915

issn

1432-0886

pii

10.1007/s00412-017-0645-6

journal_volume

127

pub_type

杂志文章
  • Random segregation of sister chromatids in developing chick retinal cells demonstrated in vivo using the fluorescence plus Giemsa technique.

    abstract::Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288461

    authors: Morris VB

    更新日期:1977-03-16 00:00:00

  • Preparation and imaging of nuclear spreads from cells of the zebrafish embryo. Evidence for large degradation intermediates in apoptosis.

    abstract::We describe a method for preparing nuclear spreads from cells of live, unfixed zebrafish embryos at the late-gastrula (approximately 8000 cell) stage of development. The method consists of a sequence of four steps: (1) a slow, gentle lysis, in low to moderate salt concentration, of cells and then nuclei, to release DN...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050280

    authors: Chan DW,Yager TD

    更新日期:1998-03-01 00:00:00

  • Divergence in centromere structure distinguishes related genomes in Coix lacryma-jobi and its wild relative.

    abstract::Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-009-0239-z

    authors: Han Y,Wang G,Liu Z,Liu J,Yue W,Song R,Zhang X,Jin W

    更新日期:2010-02-01 00:00:00

  • Cajal's contribution to the knowledge of the neuronal cell nucleus.

    abstract::In 1906, the Spanish neurobiologist Santiago Ramón y Cajal was awarded the Nobel Prize in Physiology or Medicine in recognition of his work on the structure of neurons and their connections. Cajal is commonly regarded as the father of modern neuroscience. What is less well known is that Cajal also had a great interest...

    journal_title:Chromosoma

    pub_type: 传,历史文章,杂志文章,评审

    doi:10.1007/s00412-009-0212-x

    authors: Lafarga M,Casafont I,Bengoechea R,Tapia O,Berciano MT

    更新日期:2009-08-01 00:00:00

  • Integration site preferences of endogenous retroviruses.

    abstract::Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00355364

    authors: Taruscio D,Manuelidis L

    更新日期:1991-12-01 00:00:00

  • The Robertsonian phenomenon in the house mouse: mutation, meiosis and speciation.

    abstract::Many different chromosomal races with reduced chromosome number due to the presence of Robertsonian fusion metacentrics have been described in western Europe and northern Africa, within the distribution area of the western house mouse Mus musculus domesticus. This subspecies of house mouse has become the ideal model f...

    journal_title:Chromosoma

    pub_type: 历史文章,杂志文章,评审

    doi:10.1007/s00412-014-0477-6

    authors: Garagna S,Page J,Fernandez-Donoso R,Zuccotti M,Searle JB

    更新日期:2014-12-01 00:00:00

  • The presence of an antigen reactive with a human autoantibody in Trichosia pubescens (Diptera: Sciaridae) and its association with certain transcriptionally active regions of the genome.

    abstract::The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF01735325

    authors: Amabis JM,Amabis DC,Kaburaki J,Stollar BD

    更新日期:1990-04-01 00:00:00

  • Transition from somatic to meiotic pairing and progressional changes of the synaptonemal complex in spermatocytes of Aedes aegypti.

    abstract::Aedes aegypti spermatocytes were reconstructed from electron micrographs. The species has tight somatic pairing of the chromosomes, and there are therefore no classical leptotene and zygotene stages, but rather a gradual transition from somatic pairing to meiotic pairing (= pachytene). The term "prepachytene" has been...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329808

    authors: Wandall A,Svendsen A

    更新日期:1985-01-01 00:00:00

  • Molecular characterization and cytogenetic analysis of highly repeated DNAs of lake trout, Salvelinus namaycush.

    abstract::The chromosomes of lake trout (Salvelinus namaycush) contain a considerable amount of heterochromatin located at the centromeres and/or telomeres of several chromosomes, including a sex-specific block located distally on the X chromosome. In order to investigate further the repetitive DNAs of lake trout, genomic DNA f...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352255

    authors: Reed KM,Phillips RB

    更新日期:1995-12-01 00:00:00

  • Resolving ambiguities in the karyotype of domestic sheep (Ovis aries). II. G-, Q-, and R-banded idiograms, and chromosome-specific molecular markers.

    abstract::Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF02510040

    authors: Ansari HA,Maher DW,Pearce PD,Broad TE

    更新日期:1996-07-01 00:00:00

  • Plk1 regulates mitotic Aurora A function through betaTrCP-dependent degradation of hBora.

    abstract::Polo-like kinase 1 (Plk1) and Aurora A play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Here we show that the functions of these kinases during early mitosis are coordinated through Bora, a partner of Aurora A first identified in Drosophila. Depletion of human...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-008-0165-5

    authors: Chan EH,Santamaria A,Silljé HH,Nigg EA

    更新日期:2008-10-01 00:00:00

  • Nucleolar targeting of coilin is regulated by its hypomethylation state.

    abstract::Coilin, a molecular marker for Cajal bodies (CBs), is a phosphoprotein that contains a cryptic nucleolar localization signal and multiple interacting domains, such as the RG-box. Post-translational symmetrical dimethylation of arginines on the coilin RG-box is required for the recruitment of the survival motor neuron ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-010-0276-7

    authors: Tapia O,Bengoechea R,Berciano MT,Lafarga M

    更新日期:2010-10-01 00:00:00

  • The influence of 5-azacytidine on the condensation of the short arm of rye chromosome 1R in Triticum aestivum L. root tip meristematic nuclei.

    abstract::This paper describes the effects of 5-azacytidine on the condensation state of rye (Secale cereale L.) chromatin introduced into the wheat genome (Triticum aestivum L. cv. Beaver). The wheat cultivar Beaver carries a translocation between the short arm of rye chromosome 1R (1RS) and the long arm of wheat chromosome 1B...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/pl00007688

    authors: Glyn MC,Egertová M,Gazdova B,Kovarik A,Bezdek M,Leitch AR

    更新日期:1997-12-01 00:00:00

  • Life cycle variation and regulation of macronuclear DNA content in Tetrahymena thermophila.

    abstract::The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327377

    authors: Doerder FP,DeBault LE

    更新日期:1978-10-20 00:00:00

  • A group of non-Y encoded Drosophila hydei primary spermatocyte nuclear glycoproteins exhibits epitopes depending on formation of Y chromosomal giant lampbrush loops.

    abstract::In wild-type Drosophila hydei (genotype X/Y) four different primary spermatocyte nuclear glycoproteins, classified as non-Y encoded because of their occurrence in X/O genotypes, were demonstrated to possess a few epitopes that depended on formation of the Y chromosomal giant lampbrush loops threads (th; Mr 55,000 prot...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00291050

    authors: Tischendorf G,Liebrich W,Trapitz P,Wood G,Schwochau M

    更新日期:1989-08-01 00:00:00

  • Linking replication stress with heterochromatin formation.

    abstract::The eukaryotic genome can be roughly divided into euchromatin and heterochromatin domains that are structurally and functionally distinct. Heterochromatin is characterized by its high compaction that impedes DNA transactions such as gene transcription, replication, or recombination. Beyond its role in regulating DNA a...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-015-0545-6

    authors: Nikolov I,Taddei A

    更新日期:2016-06-01 00:00:00

  • Positive and negative birefringence in chromosomes.

    abstract::By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00330371

    authors: Livolant F

    更新日期:1978-08-21 00:00:00

  • Chromosomal locations of major tRNA gene clusters of Xenopus laevis.

    abstract::In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352227

    authors: Narayanswami S,Doering JL,Fokta FJ,Rosenthal DS,Nguyen TN,Hamkalo BA

    更新日期:1995-10-01 00:00:00

  • Chromosome markers in Mus musculus: differences in C-banding between the subspecies M.m. musculus and M.m. molossinus.

    abstract::Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences we...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00294081

    authors: Dev VG,Miller DA,Tantravahi R,Schreck RR,Roderick TH,Erlanger BF,Miller OJ

    更新日期:1975-12-29 00:00:00

  • The genetics of dopa decarboxylase in Drosophila melanogaster. IV. The genetics and cytology of the 37B10-37D1 region.

    abstract::Of 204 mutations located in the 8-12 band Df(2L)130 region, 37B9-C1,2;37D1-2, 199 have been assigned to twelve lethal genes and one visible gene (hook). The 13 genes are not evenly distributed. Twelve, (possibly all thirteen) are in the seven band region 37B10-C4 giving a gene-to-band ratio of almost two. Only one gen...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286015

    authors: Wright TR,Beermann W,Marsh JL,Bishop CP,Steward R,Black BC,Tomsett AD,Wright EY

    更新日期:1981-01-01 00:00:00

  • Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.

    abstract::Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286046

    authors: Chandley AC,Hotta Y,Stern H

    更新日期:1977-07-08 00:00:00

  • Selective digestion of mouse metaphase chromosomes.

    abstract::Metaphase chromosomes prepared from colcemid-treated mouse L929 cells by non-ionic detergent lysis exhibit distinct heterochromatic centromere regions and associated kinetochores when viewed by whole mount electron microscopy. Deoxyribonuclease I treatment of these chromosomes results in the preferential digestion of ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00330554

    authors: Rattner JB,Krystal G,Hamkalo BA

    更新日期:1978-04-25 00:00:00

  • Electron microscopy of membrane-free folded chromosomes from Escherichia coli.

    abstract::Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00328479

    authors: Kavenoff R,Bowen BC

    更新日期:1976-12-16 00:00:00

  • Histone acetylation: facts and questions.

    abstract::The DNA of eukaryotic cells is organized in a complex with proteins, either as interphase chromatin or mitotic chromosomes. Nucleosomes, the structural subunits of chromatin, have long been considered as static structures, incompatible with processes occurring in chromatin. During the past few years it has become evid...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/BF00337382

    authors: Loidl P

    更新日期:1994-12-01 00:00:00

  • Structure of the fission yeast centromere cen3: direct analysis of the reiterated inverted region.

    abstract::We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00365153

    authors: Murakami S,Matsumoto T,Niwa O,Yanagida M

    更新日期:1991-12-01 00:00:00

  • The evolutionary life history of P transposons: from horizontal invaders to domesticated neogenes.

    abstract::P elements, a family of DNA transposons, are known as aggressive intruders into the hitherto uninfected gene pool of Drosophila melanogaster. Invading through horizontal transmission from an external source they managed to spread rapidly through natural populations within a few decades. Owing to their propensity for r...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s004120100144

    authors: Pinsker W,Haring E,Hagemann S,Miller WJ

    更新日期:2001-07-01 00:00:00

  • The histone chaperone Asf1 at the crossroads of chromatin and DNA checkpoint pathways.

    abstract::Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-006-0087-z

    authors: Mousson F,Ochsenbein F,Mann C

    更新日期:2007-04-01 00:00:00

  • A new type of ribonucleoprotein constituent of the polytene nucleus of the salivary glands of Chironomus thummi and Ch. tentans.

    abstract::Using electron spectroscopic imaging, a new type of small granular structural constituent has been observed in the extrachromosomal zone of the polytene nucleus of the salivary gland cells of Chironomus thummi and Chironomus tentans. These granules appear isolated or in small clumps and are often seen to be connected ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00650895

    authors: Vázquez-Nin GH,Echeverría OM,Rouelle-Rossier VB,Fakan S

    更新日期:1993-12-01 00:00:00

  • Location of nucleolar organizers in animal and plant chromosomes by means of an improved N-banding technique.

    abstract::With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00285128

    authors: Funaki K,Matsui S,Sasaki M

    更新日期:1975-01-01 00:00:00

  • Chromatin structure in the unicellular algae Olisthodiscus luteus, Crypthecodinium cohnii and Peridiniun balticum.

    abstract::Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292229

    authors: Rizzo PJ,Burghardt RC

    更新日期:1980-01-01 00:00:00