Abstract:
:BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment alone, unifilarly BrdU-substituted (TB) chromatids appeared somewhat more severely collapsed than the bifilarly substituted (BB) chromatids. Subsequent Giemsa staining, however, brought about pronounced piling up of the Giemsa dye on the TB-chromatids but not on the BB-ones, causing highly distinct differential Giemsa staining as well as a marked differentiation in surface topography between the sister chromatids. Removal of the Giemsa dye from the differentially Giemsa stained chromosomes resulted in a disappearance of such a pronounced topographic differentiation.
journal_name
Chromosomajournal_title
Chromosomaauthors
Takayama S,Utsumi KR,Sasaki Ydoi
10.1007/BF00285754subject
Has Abstractpub_date
1981-01-01 00:00:00pages
113-9issue
1eissn
0009-5915issn
1432-0886journal_volume
82pub_type
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