DNA representation of variegating heterochromatic P-element inserts in diploid and polytene tissues of Drosophila melanogaster.

Abstract:

:Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, telomeric and fourth chromosome regions. Southern blot analyses showed that the heterochromatic hsp26 transgenes are underrepresented 1.3- to 33-fold in polytene tissue relative to the endogenous euchromatic hsp26 gene. In contrast, the heterochromatic hsp26 transgenes are present in approximately the same copy number as the endogenous euchromatic hsp26 gene in diploid tissue. It appears unlikely that DNA loss could account for the lack of gene expression in diploid tissues seen with these examples of PEV.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Wallrath LL,Guntur VP,Rosman LE,Elgin SC

doi

10.1007/BF00352116

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

519-27

issue

7

eissn

0009-5915

issn

1432-0886

journal_volume

104

pub_type

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