Abstract:
:Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, telomeric and fourth chromosome regions. Southern blot analyses showed that the heterochromatic hsp26 transgenes are underrepresented 1.3- to 33-fold in polytene tissue relative to the endogenous euchromatic hsp26 gene. In contrast, the heterochromatic hsp26 transgenes are present in approximately the same copy number as the endogenous euchromatic hsp26 gene in diploid tissue. It appears unlikely that DNA loss could account for the lack of gene expression in diploid tissues seen with these examples of PEV.
journal_name
Chromosomajournal_title
Chromosomaauthors
Wallrath LL,Guntur VP,Rosman LE,Elgin SCdoi
10.1007/BF00352116subject
Has Abstractpub_date
1996-04-01 00:00:00pages
519-27issue
7eissn
0009-5915issn
1432-0886journal_volume
104pub_type
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