Abstract：
:The nature of replication origins in eukaryotic chromosomes has been examined in some detail only in yeast, Drosophila, and mammalian cells. We have used highly synchronous cultures of plasmodia of the myxomycete Physarum and two-dimensional agarose gel electrophoresis to examine replication of two developmentally controlled, early replicated genes over time in S-phase. A single, discrete origin of replication was found within 4.8 kb of the LAV1-5 gene, which encodes a homolog of profilin. In contrast, the LAV1-2 gene appears to be surrounded by several origins. Two origins were identified within a 15 kb chromosomal domain and appear to be inefficiently used. Replication forks collide at preferred sites within this domain. These terminating structures are long lived, persisting for at least 2 h of the 3 h S-phase. Analysis of restriction fragment length polymorphisms (RFLPs) within the LAV1-2 domain indicates that replication of alleles on different parental chromosomes is a highly coordinated process. Our studies of the these two early replicated, plasmodium-specific genes indicate that both a fixed, narrow origin region and a broader zone containing two closely spaced origins of DNA replication occur in Physarum.
journal_name
Chromosomajournal_title
Chromosomaauthors
Diller JD,Sauer HWdoi
10.1007/BF00368349subject
Has Abstractpub_date
1993-09-01 00:00:00pages
563-74issue
8eissn
0009-5915issn
1432-0886journal_volume
102pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract：:The location of DNA fragments representing mobile dispersed genes (MDG) in salivary gland and midgut polytene chromosomes was compared by means of in situ hybridization. In the Drosophila stock under study the average number of hybridization sites in the polytene chromosomes of one nucleus was 20 for MDG-1 and 10 for ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00285767
更新日期：1981-01-01 00:00:00
abstract：:Drosophila melanogaster was transformed with an 18 kb fragment of the C3 DNA puff of Rhynchosciara americana, including the C3-22 gene and the origins of replication that direct amplification. Different tissues and developmental stages of five independent transgenic lines were analyzed by quantitative Southern blot hy...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-003-0254-4
更新日期：2003-10-01 00:00:00
abstract：:An unstable minichromosome comprising part of the short arm of chromosome 10 of maize was recovered from an oil yellow variegated plant as a consequence of gamma irradiation of pollen. The cytological and gene dosage observations are consistent with the minichromosome being a partial isochromosome, which lags at mitot...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00352297
更新日期：1996-06-01 00:00:00
abstract：:In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-012-0363-z
更新日期：2012-06-01 00:00:00
abstract：:Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-012-0380-y
更新日期：2012-10-01 00:00:00
abstract：:Antibodies against human Rad51 protein were used to examine the distribution of Rad51 on meiotic chromatin in mouse spermatocytes and oocytes as well as chicken oocytes during sequential stages of meiosis. We observed the following dynamic changes in distribution of Rad51 during meiosis: (1) in early leptotene nuclei ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00352222
更新日期：1995-10-01 00:00:00
abstract：:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00386791
更新日期：1986-01-01 00:00:00
abstract：:In contrast to yeast, plant interphase nuclei often display incomplete alignment (cohesion) along sister chromatid arms. Sister chromatid cohesion mediated by the multi-subunit cohesin complex is essential for correct chromosome segregation during nuclear divisions and for DNA recombination repair. The cohesin complex...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-009-0220-x
更新日期：2009-10-01 00:00:00
abstract：:In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00352227
更新日期：1995-10-01 00:00:00
abstract：:We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00293338
更新日期：1989-06-01 00:00:00
abstract：:Homologous chromosomes exchange genetic information through recombination during meiosis, a process that increases genetic diversity, and is fundamental to sexual reproduction. In an attempt to shed light on the dynamics of mammalian recombination and its implications for genome organization, we have studied the recom...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-016-0624-3
更新日期：2017-10-01 00:00:00
abstract：:Recent progress in elucidating the function of synaptonemal complex (SC) proteins and of cohesins in meiocytes made possible, in particular, through the analysis of mice deficient in SC or cohesin proteins has significantly enriched our understanding of how meiotic chromosome architecture is determined. Cohesins and t...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-006-0060-x
更新日期：2006-06-01 00:00:00
abstract：:The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00293176
更新日期：1978-06-28 00:00:00
abstract：:The sub-division of S-phase in Syrian hamsters, on the basis of Brd/U/Hoechst 33258/Giemsa banding, has allowed a quantitative comparison of the replication of individual chromosome bands within defined subphases of S. This analysis has shown that in hamsters, as has been reported in humans, there are distinct pattern...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00286026
更新日期：1981-01-01 00:00:00
abstract：:The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00352202
更新日期：1991-09-01 00:00:00
abstract：:The large heterochromatic chromosome of Drosophila nasutoides reveals distinctive C- and Q-bands. The regions which are negative in C-banding appear positive in Q-banding. The isochromosomic nature of this chromosome and the locality of the satellite DNAs in this chromosome are discussed with respect to these banding ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292680
更新日期：1977-04-27 00:00:00
abstract：:Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-013-0403-3
更新日期：2013-06-01 00:00:00
abstract：:The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00293452
更新日期：1976-02-23 00:00:00
abstract：:Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focus...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-011-0328-7
更新日期：2011-10-01 00:00:00
abstract：:The salivary chromosomes of the l(3)tl mutant of D. melanogaster are considerably thicker and shorter than those of normal larvae. In most nuclei, chromosome shortening is associated with morphological changes of two types. a) The bands lose their distinctive pattern and become loose. The chromosome appears as a granu...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF01798344
更新日期：1976-04-21 00:00:00
abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00364018
更新日期：1975-11-11 00:00:00
abstract：:Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication pa...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00346006
更新日期：1992-03-01 00:00:00
abstract：:Metaphase chromosomes prepared from colcemid-treated mouse L929 cells by non-ionic detergent lysis exhibit distinct heterochromatic centromere regions and associated kinetochores when viewed by whole mount electron microscopy. Deoxyribonuclease I treatment of these chromosomes results in the preferential digestion of ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00330554
更新日期：1978-04-25 00:00:00
abstract：:The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-017-0645-6
更新日期：2018-03-01 00:00:00
abstract：:The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, sever...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-003-0241-9
更新日期：2003-07-01 00:00:00
abstract：:Chromosome behaviour during meiosis in male Syrian hamsters heterozygous for one of three translocations was analysed as part of a study of the transmission of these structural changes. Synapsis was studied using preparations of synaptonemal complexes, and chiasmate associations and the results of anaphase I segregati...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00327317
更新日期：1989-10-01 00:00:00
abstract：:We describe a method for preparing nuclear spreads from cells of live, unfixed zebrafish embryos at the late-gastrula (approximately 8000 cell) stage of development. The method consists of a sequence of four steps: (1) a slow, gentle lysis, in low to moderate salt concentration, of cells and then nuclei, to release DN...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s004120050280
更新日期：1998-03-01 00:00:00
abstract：:We studied the stability of the genomic distribution of six retrotransposon families in long-term and short-term cultures of Drosophila cells. In a subclone derived from Kc cells, no significant rearrangements were detected over an 8 year period. On the contrary, extensive reshuffling and amplification of transposon f...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292963
更新日期：1988-11-01 00:00:00
abstract：:The sex chromosomes in Sauropsida (reptiles and birds) have evolved independently many times. They show astonishing diversity in morphology ranging from cryptic to highly differentiated sex chromosomes with male (XX/XY) and female heterogamety (ZZ/ZW). Comparing such diverse sex chromosome systems thus provides unpara...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-015-0531-z
更新日期：2016-03-01 00:00:00
abstract：:Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-003-0233-9
更新日期：2003-04-01 00:00:00
