The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system.

Abstract:

:Alterations in the nuclear positioning of chromosomes and specific genes during differentiation and development have suggested strongly the existence of a relationship between non-random organization of the genome and its function. In this study, we have examined the genome organization in interphase nuclei during adipogenesis, using the pig as a model organism. We hypothesized that changes in the gene expression profile and chromatin remodeling which occur during cellular differentiation would elicit repositioning of whole chromosomes, moving specific genes on them to different regions of the nucleus. We established an in vitro adipogenesis differentiation system using mesenchymal stem cells, derived from porcine bone marrow. The nuclear position of seven adipogenesis genes (PPARG, SREBF1, FABP4, CEBPA, CEBPB, CREB, and GATA2), two control genes (SOX9 and MYL1), and six chromosomes carrying these gene loci (SSC4, SSC6, SSC12, SSC13, SSC15, and SSC17) was determined. We found that during adipogenesis, using the in vitro stem cell model system, in contrast to our original hypothesis, the nuclear position of genes involved in adipogenesis was altered radically with the up-regulation of gene expression correlating with these genes becoming more internally located within nuclei. Chromosome territories, containing these genes, were also found to alter their nuclear position during the in vitro adipogenesis model, with the most dramatic repositioning being SSC4 that moved from the nuclear periphery towards the nuclear interior. We found that during in vitro adipogenesis chromosome territories decondensed and the genes were found on loops and projections of chromatin, away from the main body of the chromosomes. From our data, it appears that the temporal repositioning of genes, emanating away from chromosomes, during adipogenesis is correlated with gene activity, supporting models of the involvement of spatial genome repositioning in regulating gene expression and the nuclear interior being an important region of the nucleus for transcription.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Szczerbal I,Foster HA,Bridger JM

doi

10.1007/s00412-009-0225-5

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

647-63

issue

5

eissn

0009-5915

issn

1432-0886

journal_volume

118

pub_type

杂志文章
  • Identification of Porto-1, a new repeated sequence that localises close to the centromere of chromosome 2 of Drosophila melanogaster.

    abstract::We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:

    authors: Coelho PA,Nurminsky D,Hartl D,Sunkel CE

    更新日期:1996-10-01 00:00:00

  • Interstrain heterochromatin polymorphisms in Drosophila melanogaster.

    abstract::Neuroblast chromosomes of 16 Drosophila melanogaster laboratory stocks (15 wild type and 1 carrying the mutant vermillion) were carefully analyzed for Q-banding patterns and morphological characteristics, in all the mitotic phases. Two forms of intraspecific heterochromatin variations, involving three types of chromos...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00399131

    authors: Halfer C

    更新日期:1981-01-01 00:00:00

  • Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.

    abstract::Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286046

    authors: Chandley AC,Hotta Y,Stern H

    更新日期:1977-07-08 00:00:00

  • A new look at the nuclear matrix.

    abstract::The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120000077

    authors: Hancock R

    更新日期:2000-07-01 00:00:00

  • Cell cycle dependent distribution of a centrosomal antigen at the perinuclear MTOC or at the kinetochores of higher plant cells.

    abstract::Compelling evidence has been obtained in favour of the idea that the nuclear surface of higher plant cells is a microtubule-nucleating and/or organizing site (MTOC), in the absence of defined centrosomes. How these plant MTOC proteins are redistributed and function during the progression of the cell cycle remains enti...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00417882

    authors: Schmit AC,Stoppin V,Chevrier V,Job D,Lambert AM

    更新日期:1994-09-01 00:00:00

  • Molecular characterization and cytogenetic analysis of highly repeated DNAs of lake trout, Salvelinus namaycush.

    abstract::The chromosomes of lake trout (Salvelinus namaycush) contain a considerable amount of heterochromatin located at the centromeres and/or telomeres of several chromosomes, including a sex-specific block located distally on the X chromosome. In order to investigate further the repetitive DNAs of lake trout, genomic DNA f...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352255

    authors: Reed KM,Phillips RB

    更新日期:1995-12-01 00:00:00

  • The action of ultraviolet light on the patterns of banding induced by restriction endonucleases in human chromosomes.

    abstract::The irradiation of metaphase spreads of human cells with ultraviolet (UV) light blocked the chromosome banding induced by Alu I, Mbo I, Dde I, Hinf I, Hae III, and Rsa I restriction endonucleases. At 13 J/m2 there was moderate inhibition of the nuclease action, which was detected as an increase in the stain intensity ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292450

    authors: Bianchi NO,Bianchi MS,Cleaver JE

    更新日期:1984-01-01 00:00:00

  • The presence of an antigen reactive with a human autoantibody in Trichosia pubescens (Diptera: Sciaridae) and its association with certain transcriptionally active regions of the genome.

    abstract::The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF01735325

    authors: Amabis JM,Amabis DC,Kaburaki J,Stollar BD

    更新日期:1990-04-01 00:00:00

  • Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.

    abstract::A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability....

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-014-0489-2

    authors: Tognetti S,Riera A,Speck C

    更新日期:2015-03-01 00:00:00

  • Closely related species of Drosophila can contain different libraries of middle repetitive DNA sequences.

    abstract::Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327329

    authors: Dowsett AP

    更新日期:1983-01-01 00:00:00

  • Fluorescent in situ hybridization (FISH) analysis of the relationship between chromosome location and nuclear morphology in human neutrophils.

    abstract::Human neutrophil nuclei typically consist of three of four large heterochromatic lobes joined by thin, DNA-containing filaments. In addition, some lobes exhibit appendages of various sizes and shapes. Classical genetic and cytological studies suggest that some appendages contain specific chromosomes. The studies repor...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050236

    authors: Aquiles Sanchez J,Karni RJ,Wangh LJ

    更新日期:1997-08-01 00:00:00

  • Chromosomal locations of major tRNA gene clusters of Xenopus laevis.

    abstract::In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00352227

    authors: Narayanswami S,Doering JL,Fokta FJ,Rosenthal DS,Nguyen TN,Hamkalo BA

    更新日期:1995-10-01 00:00:00

  • Differential basic nucleoprotein kinetics in the two kinds of Lepidoptera spermatids: nucleate (eupyrene) and anucleate (apyrene).

    abstract::Normal lepidopteran males produce two kinds of spermatozoa: nucleate (eupyrene) and anucleate (apyrene). Eupyrene spermatozoa have the usual type of elongate nuclei. But in apyrene spermatids, the nuclei never elongate and the chromatin remains in a telophase-like condition until enucleation occurs. The study of the d...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00330361

    authors: Friedländer M,Hauschteck-Jungen E

    更新日期:1982-01-01 00:00:00

  • Molecular structure of the rDNA intergenic spacer (IGS) in Triturus: implications for the hypervariability of rDNA loci.

    abstract::Ribosomal DNA (rDNA) variation in the species Triturus vulgaris meridionalis (Amphibia, Urodela) is remarkable because of unusually high intraspecific variability in the number and distribution of ribosomal loci in the karyotype; in addition, portions of the intergenic spacer (IGS) are clustered at chromosomal loci wh...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050253

    authors: De Lucchini S,Andronico F,Nardi I

    更新日期:1997-10-01 00:00:00

  • Chromosome delineation induced by a combined potassium permanganate-sodium bisulfite treatment.

    abstract::A cytological procedure for in vitro chromosome delineation has been studied using human and mouse (Mus musculus) chromosomes. This method, consisting of slide incubation in KMnO4 at 0--5 degrees C for 24 h followed by a short exposure to NaHSO3 (1--3 min) and Giemsa staining, induces extraction of chromatin from huma...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00327395

    authors: Drets ME,Novello A

    更新日期:1980-01-01 00:00:00

  • Transcriptomic and nuclear architecture of immune cells after LPS activation.

    abstract::Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focus...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-011-0328-7

    authors: Solinhac R,Mompart F,Martin P,Robelin D,Pinton P,Iannuccelli E,Lahbib-Mansais Y,Oswald IP,Yerle-Bouissou M

    更新日期:2011-10-01 00:00:00

  • Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). I. Inter- and intraspecific karyotype diversity.

    abstract::The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293452

    authors: Shaw DD

    更新日期:1976-02-23 00:00:00

  • MRE11/RAD50/NBS1: complex activities.

    abstract::The MRE11/RAD50/NBS1 complex (Mre11 complex) is a central player in most aspects of the cellular response to DNA double-strand breaks, including homologous recombination, non-homologous end joining, telomere maintenance and DNA damage checkpoint activation. Several of these findings are explained by the unusual enzyma...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-004-0306-4

    authors: Assenmacher N,Hopfner KP

    更新日期:2004-10-01 00:00:00

  • Nuclear morphology of yeast under thymidylate starvation.

    abstract::During early meiotic development the yeast Saccharomyces cerevisiae has a characteristic nuclear dense body (NDB). It is shown that the NDB can also be induced in vegetatively growing cells through the inhibition of thymidylate synthetase which causes depletion of the dTMP pool and arrests DNA synthesis. The observati...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00285759

    authors: Moens PB,Barclay BJ,Little JG

    更新日期:1981-01-01 00:00:00

  • Antibody labelling and flow cytometric analysis of metaphase chromosomes reveals two discrete structural forms.

    abstract::Metaphase chromosomes from cultured Chinese Hamster Ovary cells were labelled in suspension with a monoclonal antibody to histone 2B, counterstained with propidium iodide (PI) and analysed by flow cytometry. Contour plots of antibody binding (FITC fluorescence) against DNA content (PI fluorescence) revealed two discre...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00294783

    authors: Turner BM,Keohane A

    更新日期:1987-01-01 00:00:00

  • Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.

    abstract::Mammalian heterochromatin proteins 1 (HP1alpha, HP1beta, and HP1gamma) are nonhistone proteins that interact in vitro with a set of proteins that play a role in chromatin silencing, transcription, and chromatin remodeling. Using antibodies specific for each HP1 isoform, we showed that they segregate in distinct nuclea...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050372

    authors: Minc E,Allory Y,Worman HJ,Courvalin JC,Buendia B

    更新日期:1999-08-01 00:00:00

  • Changes in DNA topology during spermatogenesis.

    abstract::DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence micros...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288496

    authors: Risley MS,Einheber S,Bumcrot DA

    更新日期:1986-01-01 00:00:00

  • Contrasting behavior of heterochromatic and euchromatic chromosome portions and pericentric genome separation in pre-bouquet spermatocytes of hybrid mice.

    abstract::The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-014-0479-4

    authors: Scherthan H,Schöfisch K,Dell T,Illner D

    更新日期:2014-12-01 00:00:00

  • Altered distribution of MLH1 foci is associated with changes in cohesins and chromosome axis compaction in an asynaptic mutant of tomato.

    abstract::In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0363-z

    authors: Qiao H,Offenberg HH,Anderson LK

    更新日期:2012-06-01 00:00:00

  • Isolation and some properties of a mammalian ribosomal DNA.

    abstract::The DNA coding for 28 S and 18 S ribosomal RNA, including the spacer regions, has been isolated from calf (Bos taurus) thymus gland. The method used included shearing of the total DNA to a highly homogeneous size population, selective heat denaturation and S1 nuclease treatment to remove single stranded DNA. Repeated ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293439

    authors: Blin N,Stephenson EC,Stafford DW

    更新日期:1976-10-12 00:00:00

  • Quantitative sequence characterization for repetitive DNA content in the supernumerary chromosome of the migratory locust.

    abstract::Repetitive DNA is a major component in most eukaryotic genomes but is ignored in most genome sequencing projects. Here, we report the quantitative composition in repetitive DNA for a supernumerary (B) chromosome, in the migratory locust (Locusta migratoria), by Illumina sequencing of genomic DNA from B-carrying and B-...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-017-0644-7

    authors: Ruiz-Ruano FJ,Cabrero J,López-León MD,Sánchez A,Camacho JPM

    更新日期:2018-03-01 00:00:00

  • Chromosomal evolution in Callithrix emiliae.

    abstract::We studied the karyotype of specimens of Callithrix emiliae (Callithricidae, Primates) from Rondonia, Brazil. Comparison with the karyotype of Callithrix jacchus showed that, even though these two species show many karyotypic similarities, they differ by a Robertsonian translocation, a paracentric inversion and large-...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF01726697

    authors: de Souza Barros RM,Nagamachi CY,Pieczarka JC

    更新日期:1990-10-01 00:00:00

  • Chromosome structure deficiencies in MCPH1 syndrome.

    abstract::Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-015-0512-2

    authors: Arroyo M,Trimborn M,Sánchez A,Hirano T,Neitzel H,Marchal JA

    更新日期:2015-12-01 00:00:00

  • Characterization of macronuclear DNA in five species of ciliates.

    abstract::Macronuclear DNA of four hypotrichous and one holotrichous ciliate species was characterized by biochemical techniques. The renaturation kinetics of the macronuclear DNAs of all five species were similar. Repetitive sequences occur only in an amount below 2%. Although the DNA content of the macronuclei of the species ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286789

    authors: Steinbrück G,Haas I,Hellmer KH,Ammermann D

    更新日期:1981-01-01 00:00:00

  • Understanding the birth of rupture-prone and irreparable micronuclei.

    abstract::Micronuclei are extra-nuclear bodies mainly derived from ana-telophase lagging chromosomes/chromatins (LCs) that are not incorporated into primary nuclei at mitotic exit. Unlike primary nuclei, most micronuclei are enclosed by nuclear envelope (NE) that is highly susceptible to spontaneous and irreparable rupture. Rup...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-020-00741-w

    authors: Guo X,Dai X,Wu X,Zhou T,Ni J,Xue J,Wang X

    更新日期:2020-12-01 00:00:00