Unique XCI evolution in Tokudaia: initial XCI of the neo-X chromosome in Tokudaia muenninki and function loss of XIST in Tokudaia osimensis.

abstract：:Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...

journal_title：Chromosoma

authors： Crossen PE,Pathak S,Arrighi FE

更新日期：1975-11-11 00:00:00

abstract：:Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...

journal_title：Chromosoma

authors： Cahoon CK,Libuda DE

更新日期：2019-09-01 00:00:00

abstract：:The interaction of Hoechst 33258 with DNA has been examined to help clarify the mechanisms of banding. 1. In agreement with previous studies Hoechst fluorescence is enhanced to a greater degree in AT-rich compared to GC-rich DNA. 2. Hoechst causes an increase in the DNA Tm which is greater at the higher AT content of ...

journal_title：Chromosoma

authors： Comings DE

更新日期：1975-10-14 00:00:00

abstract：:The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Sat...

journal_title：Chromosoma

authors： Gisselsson D,Shao C,Tuck-Muller CM,Sogorovic S,Pålsson E,Smeets D,Ehrlich M

更新日期：2005-07-01 00:00:00

abstract：:In the XY pachytene spermatocyte, the sex chromosomes do not synapse except for the pseudoautosomal region and become transcriptionally silenced. It has been suggested that the meiotic silencing of unsynapsed chromatin (MSUC) also occurs in oocytes. In the XY sex-reversed female mouse, the sex chromosomes fail to pair...

journal_title：Chromosoma

authors： Taketo T,Naumova AK

更新日期：2013-10-01 00:00:00

abstract：:The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...

journal_title：Chromosoma

authors： Renkawitz-Pohl R

更新日期：1979-01-31 00:00:00

abstract：:A method is described for excising by a glass knife single bands of isolated polytene chromosomes of the salivary glands of Chironomus tentans larvae. DNA strands were isolated from cut-out bands and their contour lengths were determined on electron micrographs. The mean contour length of DNA strands isolated from the...

journal_title：Chromosoma

authors： Frey M,Koller T,Lezzi M

更新日期：1982-01-01 00:00:00

abstract：:To understand how microtubules interact in forming the mitotic apparatus and orienting and moving chromosomes, the precise arrangement of microtubules in kinetochore fibers in Chinese hamster ovary cells was examined. Individual microtubules were traced, using high voltage electron microscopy of serial 0.25 micron sec...

journal_title：Chromosoma

authors： Witt PL,Ris H,Borisy GG

更新日期：1981-01-01 00:00:00

abstract：:Three new 18S.26S rRNA gene loci were identified in common wheat by sequential N-banding and in situ hybridization (ISH) analysis. Locus Nor-A7 is located at the terminal area of the long arm of 5A in both diploid and polyploid wheats. Locus Nor-B6 is located in N-band 1BL2.5 of the long arm of chromosome 1B in Tritic...

journal_title：Chromosoma

authors： Jiang J,Gill BS

更新日期：1994-06-01 00:00:00

abstract：:The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...

journal_title：Chromosoma

authors： Nonomura K,Kurata N

更新日期：2001-08-01 00:00:00

abstract：:We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is charac...

journal_title：Chromosoma

authors： Jantsch M,Hamilton B,Mayr B,Schweizer D

更新日期：1990-09-01 00:00:00

abstract：:Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...

journal_title：Chromosoma

authors： Arroyo M,Trimborn M,Sánchez A,Hirano T,Neitzel H,Marchal JA

更新日期：2015-12-01 00:00:00

abstract：:Cyclins, as regulatory partners of cyclin-dependent kinases (CDKs), control the switch-like cell cycle transitions that orchestrate orderly duplication and segregation of genomes. Compared to mitosis, relatively little is known about how cyclin-CDK complexes control meiosis, the specialized cell division that generate...

journal_title：Chromosoma

authors： Karasu ME,Keeney S

更新日期：2019-09-01 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:We have characterized two post-translational histone modifications in Caenorhabditis elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional a...

journal_title：Chromosoma

authors： Gu SG,Fire A

更新日期：2010-02-01 00:00:00

abstract：:Ribosomal DNA (rDNA) variation in the species Triturus vulgaris meridionalis (Amphibia, Urodela) is remarkable because of unusually high intraspecific variability in the number and distribution of ribosomal loci in the karyotype; in addition, portions of the intergenic spacer (IGS) are clustered at chromosomal loci wh...

journal_title：Chromosoma

authors： De Lucchini S,Andronico F,Nardi I

更新日期：1997-10-01 00:00:00

abstract：:A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous...

journal_title：Chromosoma

authors： Scalenghe F,Buscaglia M,Steinheil C,Crippa M

更新日期：1978-05-16 00:00:00

abstract：:We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

journal_title：Chromosoma

authors： Lifschytz E,Hareven D

更新日期：1982-01-01 00:00:00

abstract：:The spindle checkpoint restrains anaphase onset and mitotic exit until all chromosomes are stably attached to the mitotic spindle via their kinetochores. The Tao1 protein kinase was recently reported as a novel spindle checkpoint component. When an siRNA was used to repress Tao1, the essential spindle checkpoint compo...

journal_title：Chromosoma

authors： Westhorpe FG,Diez MA,Gurden MD,Tighe A,Taylor SS

更新日期：2010-08-01 00:00:00

abstract：:Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

journal_title：Chromosoma

authors： LaFountain JR Jr

更新日期：1985-01-01 00:00:00

abstract：:Dioecious Silene latifolia evolved heteromorphic sex chromosomes within the last ten million years, making it a species of choice for studies of the early stages of sex chromosome evolution in plants. About a dozen genes have been isolated from its sex chromosomes and basic genetic and deletion maps exist for the X an...

journal_title：Chromosoma

authors： Howell EC,Armstrong SJ,Filatov DA

更新日期：2011-06-01 00:00:00

abstract：:From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...

journal_title：Chromosoma

authors： Roth GE

更新日期：1991-05-01 00:00:00

abstract：:An inverted duplication of chromosome 15 [inv dup(15)] is the most common supernumerary marker chromosome, comprising approximately 50% of all chromosomes in this class. Structurally, the inv dup(15) is a mirror image with the central axis defining a distal break within either the heterochromatic alpha-satellite array...

journal_title：Chromosoma

authors： Wandstrat AE,Schwartz S

更新日期：2000-11-01 00:00:00

abstract：:Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromoso...

journal_title：Chromosoma

authors： Angell RR,Jacobs PA

更新日期：1975-08-11 00:00:00

abstract：:During DNA synthesis there is a distinct stage immediately after the joining of large DNA replication intermediates (post-elongation stage). The conversion of this DNA to mature DNA was analysed in cells treated with aphidicolin to stop the movement of the replication fork. In such cells mature DNA is formed. In contr...

journal_title：Chromosoma

authors： Lönn U,Lönn S

更新日期：1987-01-01 00:00:00

abstract：:A system has been developed using a microscope photometer to measure the DNA content of individual chromosomes. After correcting for the systematic and statistical errors the measured values are highly reproducible. Data are presented of measurements of human Y chromosomes from a cytogenetically normal population. Whe...

journal_title：Chromosoma

authors： Wall WJ,Butler LJ

更新日期：1989-01-01 00:00:00

abstract：:The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...

journal_title：Chromosoma

authors： Shaw DD

更新日期：1976-02-23 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) technique to search the Drosophila melanogaster genome for the presence of sequences with homology to mammalian and yeast centromeric DNA. Using primers based on the human CENP-B box present in alpha-satellite DNA and part of the Saccharomyces cerevisiae CDEIII centrome...

journal_title：Chromosoma

authors： Coelho PA,Nurminsky D,Hartl D,Sunkel CE

更新日期：1996-10-01 00:00:00

abstract：:The frequency and distribution of chiasmata and the nature of terminal "associations" was re-examined in Syrian hamster spermatocytes using the 5-bromo-2'-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the pen...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1990-08-01 00:00:00

abstract：:In mammals, paternal and maternal pronuclei undergo profound chromatin reorganisation upon fertilisation. How these events are orchestrated within centromeric regions to ensure proper chromosome segregation in the following cellular divisions is unknown. In this study, we followed the dynamic unfolding of the centrome...

journal_title：Chromosoma

authors： Probst AV,Santos F,Reik W,Almouzni G,Dean W

更新日期：2007-08-01 00:00:00