Abstract:
:We have focused our research on understanding the basic biology of and developing novel therapeutic and prophylactic DNA vaccines. We have among others three distinct primary areas of interest which include: 1. Enhancing in vivo delivery and transfection of DNA vaccine vectors 2. Improving DNA vaccine construct immunogenicity 3. Using molecular adjuvants to modulate and skew immune responses. Key to the immunogenicity of DNA vaccines is the presentation of expressed antigen to antigen-presenting cells. To improve expression and presentation of antigen, we have investigated various immunization methods with current focus on a combination of intramuscular injection and electroporation. To improve our vaccine constructs, we also employed methods such as RNA/codon optimization and antigen consensus to enhance expression and cellular/humoral cross-reactivity, respectively. Our lab also researches the potential of various molecular adjuvants to skew Th1/Th2 responses, enhance cellular/humoral responses, and improve protection in various animal models. Through improving our understanding of basic immunology as it is related to DNA vaccine technology, our goal is to develop the technology to the point of utility for human and animal health.
journal_name
Immunol Resjournal_title
Immunologic researchauthors
Abdulhaqq SA,Weiner DBdoi
10.1007/s12026-008-8076-3subject
Has Abstractpub_date
2008-01-01 00:00:00pages
219-32issue
1-3eissn
0257-277Xissn
1559-0755journal_volume
42pub_type
杂志文章,评审abstract::Ethanol addition to B lymphocytes in vitro inhibits antigen-induced antibody secretion and antigen-induced cell proliferation. We tested the hypothesis that ethanol can inhibit the effect of interleukins on B-cell proliferation by adding ethanol to in vitro functional assays for IL-2 and IL-4. Ethanol does not inhibit...
journal_title:Immunologic research
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abstract::The proteins of the tumor necrosis factor (TNF) receptor superfamily are a group of cell-surface receptors critically involved in the maintenance of homeostasis of the immune system. By interacting with their corresponding ligands, these receptors either induce cell death or promote cell survival of immune cells. The ...
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更新日期:2018-12-01 00:00:00
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更新日期:2017-08-01 00:00:00
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abstract::Severe combined immunodeficiency (SCID) disorders compromise lymphocyte numbers and/or function. One subset of SCID typically affects T cell and Natural Killer (NK) cell development in tandem (T-B+NK-) due to mutations arising in the genes encoding the common γ chain or Janus Kinase 3 (JAK3). In rare circumstances, mu...
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abstract::The two-signal model of T cell activation states that antigen recognition by TCR provides a tolerogenic signal (termed Signal 1) unless the T cell receives simultaneous costimulation (Signal 2) that permits antigen recognition to prime activation. Our efforts to characterize genetic and biochemical factors resulting f...
journal_title:Immunologic research
pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
abstract::Mutations of the Wiskott-Aldrich Syndrome Protein (WASP) are responsible for classic Wiskott-Aldrich Syndrome (WAS), X-linked thrombocytopenia (XLT), and in rare instances congenital X-linked neutropenia (XLN). WASP is a regulator of actin polymerization in hematopoietic cells with well-defined functional domains that...
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更新日期:2017-02-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
doi:10.1007/s12026-013-8418-7
更新日期:2013-07-01 00:00:00
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更新日期:2010-03-01 00:00:00
abstract::Genes of the major histocompatibility complex (MHC) are one of the most significant genetic predictors of HIV and SIV disease outcome; however, in no case is MHC genotype alone sufficient to account for this durable HIV/SIV control. Killer immunoglobulin-like receptors (KIRs) interact with MHC molecules and have been ...
journal_title:Immunologic research
pub_type: 杂志文章,评审
doi:10.1007/s12026-007-8010-0
更新日期:2008-01-01 00:00:00
abstract::Natural killer cells play a major role in innate immunity against tumor and virus-infected cells. NK cells express activating and inhibitory receptors to regulate their function. It has been established that modulation in the NK cell receptor profile results in altered function of NK cell against target cells. Here, w...
journal_title:Immunologic research
pub_type: 杂志文章
doi:10.1007/s12026-017-8982-3
更新日期:2018-02-01 00:00:00
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journal_title:Immunologic research
pub_type: 杂志文章,评审
doi:10.1007/BF02786325
更新日期:1997-02-01 00:00:00
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doi:10.1007/s12026-009-8136-3
更新日期:2010-07-01 00:00:00
abstract::Type I diabetes (T1D) is a chronic autoimmune disease caused by pancreatic β-cell destruction induced by autoantibodies and autoreactive T cells. After significant reduction of the β-cell mass, diabetes sets in and can cause significant complications. It is estimated that more than 3 million Americans have T1D, and it...
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pub_type: 杂志文章,评审
doi:10.1007/s12026-014-8546-8
更新日期:2014-08-01 00:00:00
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更新日期:2006-01-01 00:00:00
abstract::Anti-CD3 monoclonal antibodies (MAbs) were developed as a way of inducing immune suppression of T cells. More recent studies have indicated that anti-CD3 MAbs can affect immune responses by inducing immune regulation. We recently reported that a single course of treatment with a non-FcR binding anti-CD3 MAb, hOKT3gamm...
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更新日期:2003-01-01 00:00:00
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journal_title:Immunologic research
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更新日期:1986-01-01 00:00:00
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更新日期:2005-01-01 00:00:00
abstract::We have recently established new bone marrow transplantation (BMT) methods for the treatment of intractable diseases. The methods include the perfusion method (PM) for the collection of bone marrow cells, and intra-bone marrow (IBM)-BMT for the direct injection of collected whole bone marrow cells into the bone marrow...
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更新日期:2007-01-01 00:00:00
abstract::Allogeneic hematopoietic cell transplantation has developed into a most successful form of immunotherapy for hematologic malignancies in the past 50 years. However, its effectiveness and wider applications have been greatly limited by the development of graft-versus-host disease (GVHD), a potentially lethal side effec...
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更新日期:2011-04-01 00:00:00
abstract::The Caenorhabditis elegans model can be used to study Candida albicans virulence and host immunity, as well as to identify plant-derived natural products to use against C. albicans. Thymol is a hydrophobic phenol compound from the aromatic plant thyme. In this study, the in vitro data demonstrated concentration-depend...
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更新日期:2016-08-01 00:00:00
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abstract::Systemic sclerosis (SSc) is an intractable multifaceted disease with high mortality. Although its pathogenesis is not fully understood, recent studies have advanced our knowledge on SSc. The cardinal pathological features of SSc are autoimmunity, vasculopathy, and fibrosis. The B cells in SSc are constitutively activa...
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doi:10.1007/s12026-017-8926-y
更新日期:2017-08-01 00:00:00
abstract::It has long been known that T cells have the potential to modulate hematopoietic response in different ways. More recently, the importance of interleukin (IL)-17-secreting Th17 cells in T-cell-mediated regulation of hematopoiesis was indicated by the line of evidence that IL-17 links T-cell function and hematopoiesis ...
journal_title:Immunologic research
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更新日期:2012-04-01 00:00:00