Abstract:
:Patterned regular sieves and filters with comparable molecular dimensions hold great promise as an alternative to conventional polymeric gels and fibrous membranes to improve biomolecule separation. Recent developments of microfabricated nanofluidic sieves and filters have demonstrated superior performance for both analytical and preparative separation of various physiologically relevant macromolecules, including proteins. The insights gained from designing these artificial molecular sieves and filters, along with the promising results gathered from their first applications, serve to illustrate the impact that they can have on improving future separation of complex biological samples. Further development of artificial sieves and filters with more elaborate geometrical constraints and tailored surface functionality is believed to provide more promising ideals and results for biomolecule separation, which has great implications for proteomic research and biomarker discovery.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Fu J,Mao P,Han Jdoi
10.1016/j.tibtech.2008.02.009subject
Has Abstractpub_date
2008-06-01 00:00:00pages
311-20issue
6eissn
0167-7799issn
1879-3096pii
S0167-7799(08)00101-7journal_volume
26pub_type
杂志文章,评审abstract::Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2004.02.003
更新日期:2004-04-01 00:00:00
abstract::In vitro selection from molecular libraries has rapidly come of age as a protein-engineering tool. Dramatic increases in protein affinity can be engineered using phage-display libraries, and specific antibodies can be selected directly from a single 'naïve' library of their genes. Repertoires of small molecules are a ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(94)90079-5
更新日期:1994-05-01 00:00:00
abstract::Psychrophilic anaerobic treatment is an attractive option for wastewaters that are discharged at moderate to low temperature. The expanded granular sludge bed (EGSB) reactor has been shown to be a feasible system for anaerobic treatment of mainly soluble and pre-acidified wastewater at temperatures of 5--10 degrees C....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(01)01701-2
更新日期:2001-09-01 00:00:00
abstract::Attempts through somatic cell nuclear transfer to expand wild populations that have shrunk to critical numbers is a logical extension of the successful cloning of mammals. However, although the first mammal was cloned 10 years ago, nuclear reprogramming remains phenomenological, with abnormal gene expression and epige...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.03.007
更新日期:2007-05-01 00:00:00
abstract::The unicellular alga Dunaliella salina is the most interesting cell factory for the commercial production of beta-carotene because this species accumulates carotenoids to high concentrations. Nevertheless, little is known about the underlying mechanisms of carotenoid accumulation. Here, we review the regulatory mechan...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2008.07.002
更新日期:2008-11-01 00:00:00
abstract::In this article, the current status of membrane bioreactor (MBR) technology for wastewater treatment is reviewed. Fundamental facets of the MBR process and membrane and process configurations are outlined and the advantages and disadvantages over conventional suspended growth-based biotreatment are briefly identified....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.11.005
更新日期:2008-02-01 00:00:00
abstract::Current biopharmaceutical manufacturing strongly relies on using purification platform processes, offering harmonization of practices and speed-to-market. However, the ability of such processes to respond quickly to anticipated higher quality and capacity demands is under question. Here, we describe novel approaches f...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2009.09.002
更新日期:2009-12-01 00:00:00
abstract::Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2013.06.007
更新日期:2013-10-01 00:00:00
abstract::The tricarboxylic acid (TCA) cycle has been used for decades in the microbial production of chemicals such as citrate, L-glutamate, and succinate. Maximizing yield is key for cost-competitive production. However, for most TCA cycle products, the maximum pathway yield is lower than the theoretical maximum yield (Y(E))....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2015.11.002
更新日期:2016-03-01 00:00:00
abstract::Genetic engineering methods based on the use of transgenes have been successfully adopted to improve crops. A novel all-native DNA gene technology consists of the creation of intragenic constructs by isolating genetic elements from a crop, rearranging them in vitro, and inserting them back into the plant. The ever-inc...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.07.005
更新日期:2012-02-01 00:00:00
abstract::The effective management of emerging insect-borne disease is dependent on the use of safe and efficacious chemical insecticides. Given the inherent ability of insects to develop resistance, it is essential to propose innovative strategies because insecticides remain the most important element of integrated approaches ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2008.12.005
更新日期:2009-04-01 00:00:00
abstract::Chronic diseases, particularly malignancies and immune-mediated inflammatory diseases (IMIDs), are a challenging frontier for clinical diagnosis and treatment, as well as for biomedical research. Current treatment regimens are frequently insufficient and thus new treatment strategies are needed. Novel therapies for di...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2004.04.001
更新日期:2004-06-01 00:00:00
abstract::Nanotechnology is currently driving the dental materials industry to substantial growth, thus reflecting on improvements in materials available for oral prevention and treatment. The present review discusses new developments in nanotechnology applied to dentistry, focusing on the use of nanomaterials for improving the...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2015.09.005
更新日期:2015-11-01 00:00:00
abstract::Soft biofunctional and biocompatible interfaces on solids designed by the deposition of ultrathin soft polymer films or supported membranes have numerous scientific and practical applications. These include the immobilization of glycolipids, membrane receptors and proteins to generate models of cell and tissue surface...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(99)01412-2
更新日期:2000-02-01 00:00:00
abstract::The extensive use of chemical insecticides for insect pest management has resulted in insecticide resistance now being recorded in >500 species of insects and mites. Although gut-active toxins such as those derived from Bacillus thuringiensis (Bt) have been successfully used for insect pest management, a diverse range...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2013.08.001
更新日期:2014-02-01 00:00:00
abstract::Continued progress in cell-phone devices has made them powerful mobile computers, equipped with sophisticated, permanent physical sensors embedded as the default configuration. By contrast, the incorporation of permanent biosensors in cell-phone units has been prevented by the multivocal nature of the stimuli and the ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2014.03.007
更新日期:2014-07-01 00:00:00
abstract::Cyanobacteria promise to be an important industrial platform for the production of a variety of biobased products such as fuels, plastics, and isoprenoids. Recent advances in synthetic biology have resulted in various cyanobacterial strain improvements. Nevertheless, these new strains are still hampered by product inh...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.11.006
更新日期:2019-05-01 00:00:00
abstract::Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosy...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2019.12.008
更新日期:2020-07-01 00:00:00
abstract::A major goal of material science is to produce hierarchical materials that are ordered on all length scales, from the molecular (1-100 A) via the nano (10-100 nm) to the meso (1-100 microm). In these materials, the larger-scale properties can be controlled by choosing molecular characteristics. Methods developed to pr...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/s0167-7799(00)01482-7
更新日期:2000-09-01 00:00:00
abstract::Gastrointestinal cancers rank second in overall cancer-related deaths. Carotenoids, sulfated polysaccharides, and polyunsaturated fatty acids (PUFAs) from microalgae exhibit cancer chemopreventive features at different stages of carcinogenesis. For instance, sulfated polysaccharides bear a prophylactic potential via b...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.11.004
更新日期:2013-02-01 00:00:00
abstract::Flow cytometry is a rapid method for the quantitative measurement of light scattering and fluorescent properties of cells. Although this technique has been widely applied to biomedical and environmental studies, its potential as a tool in ecotoxicological studies has not yet been fully exploited. This article describe...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(01)01924-2
更新日期:2002-04-01 00:00:00
abstract::Atherosclerosis and heart disease are still the leading causes of morbidity and mortality worldwide. The lack of suitable autologous grafts has produced a need for artificial grafts but the patency of such grafts is limited compared to natural materials. Tissue engineering, whereby living tissue replacements can be co...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2005.06.006
更新日期:2005-09-01 00:00:00
abstract::With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro - disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.03.008
更新日期:2018-09-01 00:00:00
abstract::Wearable sensors have garnered considerable recent interest owing to their tremendous promise for a plethora of applications. Yet the absence of reliable non-invasive chemical sensors has greatly hindered progress in the area of on-body sensing. Electrochemical sensors offer considerable promise as wearable chemical s...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2014.04.005
更新日期:2014-07-01 00:00:00
abstract::The endothelium lining blood vessels expresses molecules that are restricted in their expression to a particular tissue or organ. These molecules are attractive targets for therapy and diagnosis because they allow agents to be delivered specifically to the blood vessels supplying the desired tissue. However, it is dif...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/S0167-7799(03)00079-9
更新日期:2003-05-01 00:00:00
abstract::Many organisms have the ability to form spores, a remarkable phase in their life cycles. Compared with vegetative cells, spores have several advantages (e.g. resistance to toxic compounds, temperature, desiccation and radiation) making them well suited to various applications. The applications of spores that first spr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(03)00170-7
更新日期:2003-08-01 00:00:00
abstract::Transgenic plants that express foreign proteins with industrial or pharmaceutical value represent an economical alternative to fermentation-based production systems. Specific vaccines have been produced in plants as a result of the transient or stable expression of foreign genes. It has recently been shown that genes ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(00)88986-6
更新日期:1995-09-01 00:00:00
abstract::A recent publication has reported the fabrication of a new microbial culture device containing an array of unprecedented density - a million growth chambers. This micro-Petri chip should impact on various fields, including biotechnology, ecology, food microbiology and drug development, by enabling high-throughput scre...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2008.03.007
更新日期:2008-07-01 00:00:00
abstract::High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated pr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.08.002
更新日期:2019-01-01 00:00:00
abstract::The expression 'crystal clear' stems from the science of crystallography, which determines the detailed atomic structures of materials by exposing crystals to X-rays. Protein structures are pivotal to the success of rational drug design and other biotechnology applications; however, obtaining high quality crystals pos...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2008.10.008
更新日期:2009-02-01 00:00:00