Abstract：
:The extensive use of chemical insecticides for insect pest management has resulted in insecticide resistance now being recorded in >500 species of insects and mites. Although gut-active toxins such as those derived from Bacillus thuringiensis (Bt) have been successfully used for insect pest management, a diverse range of insect-specific insecticidal peptides remains an untapped resource for pest management efforts. These toxins act within the insect hemocoel (body cavity) and hence require a delivery system to access their target site. Here, we summarize recent developments for appropriate delivery of such intrahemocoelic insect toxins, via fusion to a second protein such as a plant lectin or a luteovirus coat protein for transcytosis across the gut epithelium, or via entomopathogenic fungi.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Bonning BC,Chougule NPdoi
10.1016/j.tibtech.2013.08.001subject
Has Abstractpub_date
2014-02-01 00:00:00pages
91-8issue
2eissn
0167-7799issn
1879-3096pii
S0167-7799(13)00177-7journal_volume
32pub_type
杂志文章,评审abstract：:Continued progress in cell-phone devices has made them powerful mobile computers, equipped with sophisticated, permanent physical sensors embedded as the default configuration. By contrast, the incorporation of permanent biosensors in cell-phone units has been prevented by the multivocal nature of the stimuli and the ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2014.03.007
更新日期：2014-07-01 00:00:00
abstract：:Taxol® (generic name paclitaxel) represents one of the most clinically valuable natural products known to mankind in the recent past. More than two decades have elapsed since the notable discovery of the first Taxol®-producing endophytic fungus, which was followed by a plethora of reports on other endophytes possessin...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2014.03.011
更新日期：2014-06-01 00:00:00
abstract：:A key aspect of the genomics revolution is the transformation of large amounts of biological information into an electronic format, leading to an information-based approach to biomedical problems. Large-scale RNA assays and gene-expression-microarray studies, in particular, represent the second wave of the genomics re...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(99)01359-1
更新日期：1999-11-01 00:00:00
abstract：:Microalgal production technologies are seen as increasingly attractive for bioenergy production to improve fuel security and reduce CO(2) emissions. Photosynthetically derived fuels are a renewable, potentially carbon-neutral and scalable alternative reserve. Microalgae have particular promise because they can be prod...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.11.003
更新日期：2012-04-01 00:00:00
abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.12.014
更新日期：2016-07-01 00:00:00
abstract：:Innovative fermentation processes are necessary for the cost-effective production of bulk chemicals from renewable resources. Current microbial processes are either anaerobic processes, with high yield and productivity, or less-efficient aerobic processes. Oxygen utilization plays an important role in energy generatio...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2010.12.007
更新日期：2011-04-01 00:00:00
abstract：:Microbial exopolysaccharides such as xanthan and dextran have been commercial products for many years; the search for new gelling agents has yielded gellan. Exopolysaccharides have many other novel properties to offer, and the discovery of immune modulation and tumouristasis by beta-D-glucans, and the use of bacterial...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(97)01139-6
更新日期：1998-01-01 00:00:00
abstract：:The analysis of biomolecules using microarrays and other biosensors has a significant role in molecular biotechnology, and will become even more important in the future as a versatile tool for research and diagnostics. For many applications, the synthetic DNA mimic peptide nucleic acid (PNA) could be advantageous as a...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2004.10.003
更新日期：2004-12-01 00:00:00
abstract：:Microfluidic devices offer automation and high-throughput screening, and operate at low volumes of consumables. Although microfluidics has the potential to reduce turnaround times and costs for analytical devices, particularly in medical, veterinary, and environmental sciences, this enabling technology has had limited...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2014.04.010
更新日期：2014-07-01 00:00:00
abstract：:A major goal of material science is to produce hierarchical materials that are ordered on all length scales, from the molecular (1-100 A) via the nano (10-100 nm) to the meso (1-100 microm). In these materials, the larger-scale properties can be controlled by choosing molecular characteristics. Methods developed to pr...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/s0167-7799(00)01482-7
更新日期：2000-09-01 00:00:00
abstract：:Atherosclerosis and heart disease are still the leading causes of morbidity and mortality worldwide. The lack of suitable autologous grafts has produced a need for artificial grafts but the patency of such grafts is limited compared to natural materials. Tissue engineering, whereby living tissue replacements can be co...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2005.06.006
更新日期：2005-09-01 00:00:00
abstract：:Modern biocatalysis is developing new and precise tools to improve a wide range of production processes, which reduce energy and raw material consumption and generate less waste and toxic side-products. Biocatalysis is also achieving new advances in environmental fields, from enzymatic bioremediation to the synthesis ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.04.002
更新日期：2006-06-01 00:00:00
abstract：:Two of the primary responses in a cell when perturbed are modulation of the dynamics of its constituent gene expression and protein abundance to restore steady-state homeostasis. To obtain a detailed model of the restoration of this balance, it is necessary to examine the kinetics of transcription and translation, thu...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2012.09.007
更新日期：2012-12-01 00:00:00
abstract：:The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(90)90147-p
更新日期：1990-04-01 00:00:00
abstract：:Genetic engineering methods based on the use of transgenes have been successfully adopted to improve crops. A novel all-native DNA gene technology consists of the creation of intragenic constructs by isolating genetic elements from a crop, rearranging them in vitro, and inserting them back into the plant. The ever-inc...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.07.005
更新日期：2012-02-01 00:00:00
abstract：:Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosy...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2019.12.008
更新日期：2020-07-01 00:00:00
abstract：:Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed, with ever-improving simplicity, efficiency, and applicability. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2020.06.010
更新日期：2021-02-01 00:00:00
abstract：:Anywhere water is in the liquid state, bacteria will exist as biofilms, which are complex communities of cells that are cemented together. Although frequently associated with disease and biofouling, biofilms are also important for engineering applications, such as bioremediation, biocatalysis and microbial fuel cells....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.11.001
更新日期：2011-02-01 00:00:00
abstract：:Allergenicity prediction is one of the most challenging aspects in the safety assessment of foods derived from either biotechnology or novel food proteins. Here we present a bottom-up strategy that defines a priori the specific risk assessment (RA) needs based on a database appropriately built for such purposes. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2020.05.011
更新日期：2021-01-01 00:00:00
abstract：:The glycosylation profile of a recombinant glycoprotein can not only significantly affect its therapeutic profile, but is also extremely sensitive to cell-culture and purification conditions. To define glycosylation patterns and to ensure consistency of recombinant glycoproteins among different preparations requires h...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(92)90244-p
更新日期：1992-08-01 00:00:00
abstract：:Here, we discuss recent advances in the development of artificial red blood cell (RBC) substitutes, illustrating lessons learned from initial attempts using perfluorocarbon (PFC) emulsions and acellular hemoglobin-based oxygen carriers (HBOCs). We also highlight novel oxygen-containing microparticles, nanoparticles, a...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2014.05.001
更新日期：2014-09-01 00:00:00
abstract：:Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(93)90097-S
更新日期：1993-09-01 00:00:00
abstract：:Attempts through somatic cell nuclear transfer to expand wild populations that have shrunk to critical numbers is a logical extension of the successful cloning of mammals. However, although the first mammal was cloned 10 years ago, nuclear reprogramming remains phenomenological, with abnormal gene expression and epige...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2007.03.007
更新日期：2007-05-01 00:00:00
abstract：:Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2004.02.003
更新日期：2004-04-01 00:00:00
abstract：:Non-natural, functional RNA molecules, such as short interfering (si) RNAs, aptazymes, maxizymes and intramers, allow modulation of gene function at the mRNA or protein level. This review discusses recent advances made in the expression and application of these functional RNAs and illustrates how engineered, intracell...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(02)02063-2
更新日期：2002-11-01 00:00:00
abstract：:In vitro antibody generation technologies have now been available for two decades. Research reagents prepared via phage display are becoming available and several recent studies have demonstrated that these technologies are now sufficiently advanced to facilitate generation of a comprehensive renewable resource of ant...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2010.05.001
更新日期：2010-07-01 00:00:00
abstract：:Are current docking methods capable of building complexes from putative component protein structures? Results of recent computational studies, including those of the CAPRI (Critical Assessment of Protein Interactions) competition, were used to determine the key properties for successful docking and introduce a classif...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2004.01.006
更新日期：2004-03-01 00:00:00
abstract：:The free-radical gas nitric oxide (NO) plays an important role in a wide and diverse range of physiological processes. As progress is made in understanding the biological function of NO, there is growing interest in the possibility that inhibitors of NO synthase (NOS) may be of clinical use in the therapy of certain d...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(00)88908-8
更新日期：1995-02-01 00:00:00
abstract：:Recent innovations in the materials used for bioprinting have enabled transformative gains in the resolution and architecture of 3D-printed engineered tissues. We focus here on one of these innovations, reported by Lee et al., which lowers the resolution limit for printing soft biomaterials. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2019.09.003
更新日期：2019-11-01 00:00:00
abstract：:The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this 'post-genomic'...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.06.003
更新日期：2016-12-01 00:00:00
