Abstract:
:The tricarboxylic acid (TCA) cycle has been used for decades in the microbial production of chemicals such as citrate, L-glutamate, and succinate. Maximizing yield is key for cost-competitive production. However, for most TCA cycle products, the maximum pathway yield is lower than the theoretical maximum yield (Y(E)). For succinate, this was solved by creating two pathways to the product, using both branches of the TCA cycle, connected by the glyoxylate shunt (GS). A similar solution cannot be applied directly for production of compounds from the oxidative branch of the TCA cycle because irreversible reactions are involved. Here, we describe how this can be overcome and what the impact is on the yield.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Vuoristo KS,Mars AE,Sanders JPM,Eggink G,Weusthuis RAdoi
10.1016/j.tibtech.2015.11.002subject
Has Abstractpub_date
2016-03-01 00:00:00pages
191-197issue
3eissn
0167-7799issn
1879-3096pii
S0167-7799(15)00242-5journal_volume
34pub_type
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