Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology.

abstract：:Hydrophobic charge induction chromatography using 4-mercapto-ethyl-pyridine as the ligand is an effective method for the separation of antibodies from a variety of feedstocks. Antibodies are adsorbed in physiological conditions without preliminary concentration. Desorption occurs when the pH is lowered, thus inducing ...

journal_title：Trends in biotechnology

authors： Boschetti E

更新日期：2002-08-01 00:00:00

abstract：:Rising global demand for meat will result in increased environmental pollution, energy consumption, and animal suffering. Cultured meat, produced in an animal-cell cultivation process, is a technically feasible alternative lacking these disadvantages, provided that an animal-component-free growth medium can be develop...

journal_title：Trends in biotechnology

authors： van der Weele C,Tramper J

更新日期：2014-06-01 00:00:00

abstract：:A major impact of genome projects on human health will be their contribution to the understanding of protein function. Proteins are the engines of biological systems, nearly all pharmaceuticals act on proteins and increasingly proteins themselves are used therapeutically. As biology enters the post-genomic era, resear...

journal_title：Trends in biotechnology

authors： Braun P,LaBaer J

更新日期：2003-09-01 00:00:00

abstract：:Cultured mammalian cells are the main workhorses for producing biologics. The performance of these cell culture processes, in terms of both productivity and product quality attributes, is significantly influenced by cellular metabolism. Glucose is the major carbon source for cellular biosynthesis and energy generation...

journal_title：Trends in biotechnology

authors： Mulukutla BC,Yongky A,Le T,Mashek DG,Hu WS

更新日期：2016-08-01 00:00:00

abstract：:Targeting apoptotic cell death pathways provides wide-ranging opportunities for the discovery and development of novel drugs. Some targeted therapies that selectively induce apoptosis in cancer cells are already marketed, and numerous pro-apoptotic drugs for treating cancer are currently being developed. The anti-apop...

journal_title：Trends in biotechnology

authors： Alam JJ

更新日期：2003-11-01 00:00:00

abstract：:Advances in our capacity to design and use novel strategies for achieving inducible gene expression will improve our ability to define gene function. An extremely efficient system designed by nature -- that of the regulatable phytochrome system in plants -- has provided the basis for developing a novel inducible gene ...

journal_title：Trends in biotechnology

authors： Keyes WM,Mills AA

更新日期：2003-02-01 00:00:00

abstract：:Although many microorganisms have been used for the bioindustrial generation of valuable metabolites, the productive potential of cyanobacterial species has remained largely unexplored. Cyanobacteria possess several advantages as organisms for bioindustrial processes, including simple input requirements, tolerance of ...

journal_title：Trends in biotechnology

authors： Ducat DC,Way JC,Silver PA

更新日期：2011-02-01 00:00:00

abstract：:The application of recombinant-DNA methods for the production of therapeutic proteins has, over the past decade, driven the development of new technology for the analysis and characterization of biological molecules. High performance capillary electrophoresis (HPCE) has generated enormous interest among biochemists, a...

journal_title：Trends in biotechnology

authors： Frenz J,Hancock WS

更新日期：1991-07-01 00:00:00

abstract：:Technology is a social practice that embodies the capacity of societies to transform themselves by creating and manipulating not only physical objects, but also symbols and cultural forms. It is an illusion that scientific and socioeconomic drivers are the sole elements determining the destiny of a technology. Althoug...

journal_title：Trends in biotechnology

authors： Mordini E

更新日期：2007-12-01 00:00:00

abstract：:We describe factors promoting the exploration of locally sourced probiotics, targeting local populations to balance human needs and market opportunities. This would be particularly beneficial for people in developing countries, who generally lack access to affordable probiotics and are often exposed to poor hygiene co...

journal_title：Trends in biotechnology

authors： Sybesma W,Kort R,Lee YK

更新日期：2015-04-01 00:00:00

abstract：:The unicellular alga Dunaliella salina is the most interesting cell factory for the commercial production of beta-carotene because this species accumulates carotenoids to high concentrations. Nevertheless, little is known about the underlying mechanisms of carotenoid accumulation. Here, we review the regulatory mechan...

journal_title：Trends in biotechnology

authors： Lamers PP,Janssen M,De Vos RC,Bino RJ,Wijffels RH

更新日期：2008-11-01 00:00:00

abstract：:Recent advances in the extraction and purification of decellularized extracellular matrix (dECM) obtained from healthy or malignant tissues open new avenues for engineering physiomimetic 3D in vitro tumor models, which closely recapitulate key biomolecular hallmarks and the dynamic cancer cell-ECM interactions in the ...

journal_title：Trends in biotechnology

authors： Ferreira LP,Gaspar VM,Mano JF

更新日期：2020-12-01 00:00:00

abstract：:Most infections are caused by bacteria, many of which are ever-evolving and resistant to nearly all available antibiotics. β-Lactams and glycopeptides are used to combat these infections by inhibiting bacterial cell-wall synthesis. This mechanism remains an interesting target in the search for new antibiotics in light...

journal_title：Trends in biotechnology

authors： Jovetic S,Zhu Y,Marcone GL,Marinelli F,Tramper J

更新日期：2010-12-01 00:00:00

abstract：:Luminescence has assumed an important role in analytical biochemistry and molecular biology as an extremely sensitive method for determining the concentration of specific ions and molecules. The luminescent system of the jellyfish Aequorea victoria consists of the photoprotein aequorin, which contains the molecule coe...

journal_title：Trends in biotechnology

authors： Jones K,Hibbert F,Keenan M

更新日期：1999-12-01 00:00:00

abstract：:Allergenicity prediction is one of the most challenging aspects in the safety assessment of foods derived from either biotechnology or novel food proteins. Here we present a bottom-up strategy that defines a priori the specific risk assessment (RA) needs based on a database appropriately built for such purposes. ...

journal_title：Trends in biotechnology

authors： Fernandez A,Mills ENC,Koning F,Moreno FJ

更新日期：2021-01-01 00:00:00

abstract：:The well-studied antigen-combining sites of antibody molecules hold considerable promise as a model system for the design of bioactive peptides. These small, immunoglobulin-derived peptides can be used in the development of alternative treatments for disease and in diagnostic strategies. The general principles derived...

journal_title：Trends in biotechnology

authors： Dougall WC,Peterson NC,Greene MI

更新日期：1994-09-01 00:00:00

abstract：:Microbial contamination of polymer surfaces has become a significant challenge in domestic, industrial, and biomedical applications. Recent progress in our understanding of how topographical features of different length scales can be used to effectively and selectively control the attachment and proliferation of diffe...

journal_title：Trends in biotechnology

authors： Nguyen DHK,Bazaka O,Bazaka K,Crawford RJ,Ivanova EP

更新日期：2020-05-01 00:00:00

abstract：:The general acceptance of bioremediation technology as an environmentally sound and economic treatment for hazardous waste requires the demonstration of its efficacy, reliability and predictability, as well as its advantages over conventional treatments. An effective monitoring design includes protocols for treatment-...

journal_title：Trends in biotechnology

authors： Heitzer A,Sayler GS

更新日期：1993-08-01 00:00:00

abstract：:Genomic research is expected to generate new types of complex observational data, changing the types of experiments as well as our understanding of biological processes. The investigation and definition of relationships among proteins is essential for understanding the function of each gene and the mechanisms of biolo...

journal_title：Trends in biotechnology

authors： Haberkorn U,Altmann A

更新日期：2003-06-01 00:00:00

abstract：:Nature's most potent molecules are produced by enzyme-catalysed reactions, coupled with the natural selection of those products that possess optimal biological activity. Combinatorial biocatalysis harnesses the natural diversity of enzymatic reactions for the iterative synthesis of organic libraries. Iterative reactio...

journal_title：Trends in biotechnology

authors： Michels PC,Khmelnitsky YL,Dordick JS,Clark DS

更新日期：1998-05-01 00:00:00

abstract：:Techniques such as positron-emission tomography, single-photon-emission computed tomography, functional magnetic-resonance imaging and magnetoencephalography permit the observation of biological processes in the brain in a noninvasive manner. They have yielded new insights into the biological interrelations of sensory...

journal_title：Trends in biotechnology

authors： Müller-Gärtner HW

更新日期：1998-03-01 00:00:00

abstract：:The discovery of targets that are sufficiently robust to yield marketable therapeutics is an enormous challenge. Through the years, several approaches have been used with varying degrees of success. These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to i...

journal_title：Trends in biotechnology

authors： Ryan TE,Patterson SD

更新日期：2002-12-01 00:00:00

abstract：:Genetic engineering methods based on the use of transgenes have been successfully adopted to improve crops. A novel all-native DNA gene technology consists of the creation of intragenic constructs by isolating genetic elements from a crop, rearranging them in vitro, and inserting them back into the plant. The ever-inc...

journal_title：Trends in biotechnology

authors： Molesini B,Pii Y,Pandolfini T

更新日期：2012-02-01 00:00:00

abstract：:Chimeric antigen receptor (CAR) technology and its application to regulatory T cells (Tregs) has garnered interest among researchers in the field of cell and gene therapy. Merging the benefits of CAR technology with Tregs offers a novel and promising therapeutic option for durable reshaping of undesired immune respons...

journal_title：Trends in biotechnology

authors： Fritsche E,Volk HD,Reinke P,Abou-El-Enein M

更新日期：2020-10-01 00:00:00

abstract：:The extensive use of chemical insecticides for insect pest management has resulted in insecticide resistance now being recorded in >500 species of insects and mites. Although gut-active toxins such as those derived from Bacillus thuringiensis (Bt) have been successfully used for insect pest management, a diverse range...

journal_title：Trends in biotechnology

authors： Bonning BC,Chougule NP

更新日期：2014-02-01 00:00:00

abstract：:Along with the development of metabolic engineering and synthetic biology tools, various microbes are being used to produce aromatic chemicals. In microbes, aromatics are mainly produced via a common important precursor, chorismate, in the shikimate pathway. Natural or non-natural aromatics have been produced by engin...

journal_title：Trends in biotechnology

authors： Noda S,Kondo A

更新日期：2017-08-01 00:00:00

abstract：:Reconstituted viral envelopes (RVEs) are formed by solubilizing intact virus in detergent and reassembling the envelope on removal of detergent. RVEs can be formed in the presence of agents that become encapsulated and can then be utilized in vitro and in vivo for drug delivery, cell destruction, transfer of membrane ...

journal_title：Trends in biotechnology

authors： Blumenthal R,Loyter A

更新日期：1991-02-01 00:00:00

abstract：:Traditionally, biotechnology design has focused on the manufacture of chemicals and biologics. Still, a majority of biotechnology products that appear on the market today is the result of mechanical-electric (mechatronic) construction. For these, the biological components play decisive roles in the design solution; th...

journal_title：Trends in biotechnology

authors： Mandenius CF,Björkman M

更新日期：2010-05-01 00:00:00

abstract：:The need for companion diagnostics, point-of-care testing (POCT) and high-throughput screening in clinical diagnostics and personalized medicine has pushed the need for more biological information from a single sample at extremely low concentrations and volumes. Optical biosensors based on semiconductor quantum dots (...

journal_title：Trends in biotechnology

authors： Jin Z,Hildebrandt N

更新日期：2012-07-01 00:00:00

abstract：:Pressure is a thermodynamic parameter whose unique effects on biological systems are increasingly being studied in a growing number of scientific fields. As such, the effects of high pressure are currently being investigated at different levels, ranging from proteins, enzymes and viruses to microorganisms, mammalian c...

journal_title：Trends in biotechnology

authors： Aertsen A,Meersman F,Hendrickx ME,Vogel RF,Michiels CW

更新日期：2009-07-01 00:00:00