Abstract：
:Acidity is a biomarker of cancer that is not subject to the blunting clonal selection effects that reduce the efficacy of other biomarker technologies, such as antibody targeting. The pH (low) insertion peptides (pHLIP®s) provide new opportunities for targeting acidic tissues. Through the physical mechanism of membrane-associated folding, pHLIPs are triggered by the acidic microenvironment to insert and span the membranes of tumor cells. The pHLIP platform can be applied to imaging acidic tissues, delivering cell-permeable and impermeable molecules to the cytoplasm, and promoting the cellular uptake of nanoparticles. Since acidosis is a hallmark of tumor development, progression, and aggressiveness, the pHLIP technology may prove useful in targeting cancer cells and metastases for tumor diagnosis, imaging, and therapy.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Wyatt LC,Lewis JS,Andreev OA,Reshetnyak YK,Engelman DMdoi
10.1016/j.tibtech.2017.03.014subject
Has Abstractpub_date
2017-07-01 00:00:00pages
653-664issue
7eissn
0167-7799issn
1879-3096pii
S0167-7799(17)30063-Xjournal_volume
35pub_type
杂志文章,评审abstract：:Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of t...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90046-9
更新日期：1994-08-01 00:00:00
abstract：:Regenerative medicine approaches based on decellularized extracellular matrix (ECM) scaffolds and tissues are rapidly expanding. The rationale for using ECM as a natural biomaterial is the presence of bioactive molecules that drive tissue homeostasis and regeneration. Moreover, appropriately prepared ECM is biodegrada...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2012.12.004
更新日期：2013-03-01 00:00:00
abstract：:There is a need for techniques that ensure antibiotic removal in anaerobic digesters for robust methane production. In this article, we discuss recent strategies for enhanced antibiotic removal from swine wastewater and offer insights on anaerobic digestion (AD) process design for improved antibiotic removal. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2020.07.002
更新日期：2021-01-01 00:00:00
abstract：:Breath-based metabolomics (breathomics) is an exciting developing area of biotechnology that centers on the capture, identification, and quantification of volatile organic compound (VOC) patterns in human breath and their utilization as tools in the diagnosis of a broad spectrum of medical problems. With the age of pe...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2014.08.003
更新日期：2014-10-01 00:00:00
abstract：:The development of yeast artificial chromosome (YAC) cloning vectors capable of carrying several hundred kilobase-pairs of DNA insert has greatly facilitated the study of complex genomes, and the cloning of large genes as single fragments. In addition, the ability to manipulate YAC sequences by homologous recombinatio...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(92)90165-r
更新日期：1992-01-01 00:00:00
abstract：:High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated pr...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.08.002
更新日期：2019-01-01 00:00:00
abstract：:Although microorganisms have long been known as producers of edible oils, opportunities for their biotechnological exploitation are limited to the highest-value commodities. A recent attempt to develop a yeast oil cocoa-butter equivalent has not succeeded, not because of the inability to produce the correct formulatio...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(93)90015-2
更新日期：1993-07-01 00:00:00
abstract：:Challenges in demonstrating interchangeability and safety, as well as the ongoing evolution of regulations governing biosimilars, have meant that the development of the biosimilars industry has not been, and will not be, a carbon copy of the generics industry. Complexity in the development process reduces the cost adv...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.10.009
更新日期：2016-01-01 00:00:00
abstract：:Feedstocks that deserve serious consideration for fuels and chemicals are sugarcane, corn, trees and algae. Commercialization of biomass refining is imminent but the wild claims of those who think that bioenergy can replace much of our dependence on foreign oil are appalling. It is naive to view biomass as the panacea...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2003.12.002
更新日期：2004-02-01 00:00:00
abstract：:Selective delivery of lethal levels of drugs to tumors, without concomitant damage to normal tissues, is a major challenge in cancer chemotherapy. Prodrugs used in conjunction with enzyme-monoclonal antibody conjugates that can target tumors and convert prodrugs to their active drug forms in situ, offer exceptional pr...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90122-8
更新日期：1994-06-01 00:00:00
abstract：:In the wake of public distrust regarding biotechnology, it has been suggested that the debate should be moved "upstream", whereby the public help to set research priorities. Although many scientists see this as an illogical reaction to a loss of faith in science, we argue that the boundaries between science and its te...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2005.12.007
更新日期：2006-02-01 00:00:00
abstract：:Step by step, the components of an artificial form of cellular life are being assembled by researchers. Lipid vesicles the size of small bacteria can be prepared and under certain conditions are able to grow and divide, then grow again. Polymerase enzymes encapsulated in the vesicles can synthesize RNA from externally...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2005.05.008
更新日期：2005-07-01 00:00:00
abstract：:The demands on drug discovery organizations have increased dramatically in recent years, partly because of the need to identify novel targets that are both relevant to disease and chemically tractable. This is leading to an industrial approach to traditional biology and chemistry, inspired in part by the revolution in...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/s0167-7799(01)01696-1
更新日期：2001-08-01 00:00:00
abstract：:Porous microcarriers are new support materials with important advantages in both industrial cell-culture processes and the culture of cells of medical importance. Porous microcarriers are now commercially available with internal architecture and surface chemistry suitable for culture of both anchorage-dependent and an...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(90)90154-p
更新日期：1990-05-01 00:00:00
abstract：:Microalgal production technologies are seen as increasingly attractive for bioenergy production to improve fuel security and reduce CO(2) emissions. Photosynthetically derived fuels are a renewable, potentially carbon-neutral and scalable alternative reserve. Microalgae have particular promise because they can be prod...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.11.003
更新日期：2012-04-01 00:00:00
abstract：:Soft biofunctional and biocompatible interfaces on solids designed by the deposition of ultrathin soft polymer films or supported membranes have numerous scientific and practical applications. These include the immobilization of glycolipids, membrane receptors and proteins to generate models of cell and tissue surface...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(99)01412-2
更新日期：2000-02-01 00:00:00
abstract：:Bacterial genomics has provided a plethora of potential targets for antibacterial drug discovery, however, success in the hunt for new antibiotics will hinge on selecting targets with the highest potential. A recent paper by Liu and coworkers describes a new approach to target selection that uncovers strategies used b...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2004.07.004
更新日期：2004-09-01 00:00:00
abstract：:Most infections are caused by bacteria, many of which are ever-evolving and resistant to nearly all available antibiotics. β-Lactams and glycopeptides are used to combat these infections by inhibiting bacterial cell-wall synthesis. This mechanism remains an interesting target in the search for new antibiotics in light...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.09.004
更新日期：2010-12-01 00:00:00
abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.12.014
更新日期：2016-07-01 00:00:00
abstract：:Since its invention at the beginning of the 1990s, antibody phage display has revolutionized the generation of monoclonal antibodies and their engineering. It is now possible to create antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-pa...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(97)84205-9
更新日期：1997-02-01 00:00:00
abstract：:The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this 'post-genomic'...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.06.003
更新日期：2016-12-01 00:00:00
abstract：:Brain-machine interfaces (BMIs) enable humans to interact with devices by modulating their brain signals. Despite impressive technological advancements, several obstacles remain. The most commonly used BMI control signals are derived from the brain areas involved in primary sensory- or motor-related processing. Howeve...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2017.03.008
更新日期：2017-07-01 00:00:00
abstract：:Phytoremediation holds promise for in situ treatment of heavy metal contaminated soils. Recently, the benefits of combining siderophore-producing bacteria (SPB) with plants for metal removal from contaminated soils have been demonstrated. Metal-resistant SPB play an important role in the successful survival and growth...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.12.002
更新日期：2010-03-01 00:00:00
abstract：:Antibodies to surface proteins of the sexual stages of Plasmodium falciparum block completely the transmission of these malaria parasites. Transmission-blocking vaccines therefore represent a powerful and novel approach to controlling the spread of this lethal disease. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(92)90280-9
更新日期：1992-11-01 00:00:00
abstract：:Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial consortia for diverse applications, including the bioproduction of medicines, biofuels, and biomaterials from inexpe...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.11.002
更新日期：2019-02-01 00:00:00
abstract：:Opinions on what synthetic biology actually is range from a natural extension of genetic engineering to a new manufacturing paradigm. It offers, for the first time in the life sciences, rational design and engineering standardisation. It could address problems across a broad spectrum of human concerns, including energ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.01.011
更新日期：2013-05-01 00:00:00
abstract：:Reconstituted viral envelopes (RVEs) are formed by solubilizing intact virus in detergent and reassembling the envelope on removal of detergent. RVEs can be formed in the presence of agents that become encapsulated and can then be utilized in vitro and in vivo for drug delivery, cell destruction, transfer of membrane ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(91)90184-J
更新日期：1991-02-01 00:00:00
abstract：:The mapping of the human genome was completed earlier this year and efforts are underway to understand the role of gene products (i.e. proteins) in biological pathways and human disease and to exploit their functional roles to derive protein therapeutics and protein-based drugs. A key component to the next revolution ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(01)01666-3
更新日期：2001-07-01 00:00:00
abstract：:Since its inception a decade ago, sol-gel encapsulation has opened up an intriguing new way to immobilize biological materials. An array of substances, including catalytic antibodies, DNA, RNA, antigens, live bacterial, fungal, plant and animal cells and whole protozoa, have been encapsulated in silica, metal-oxide, o...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(00)01457-8
更新日期：2000-07-01 00:00:00
abstract：:The glycosylation profile of a recombinant glycoprotein can not only significantly affect its therapeutic profile, but is also extremely sensitive to cell-culture and purification conditions. To define glycosylation patterns and to ensure consistency of recombinant glycoproteins among different preparations requires h...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(92)90244-p
更新日期：1992-08-01 00:00:00
