Serum-free hybridoma culture: ethical, scientific and safety considerations.

Abstract:

:Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and safety concerns. The use of FBS in hybridoma culture media is examined here, with regards to the development and production of monoclonal antibodies (mAbs), and it is our recommendation that researchers adopt serum-free cell culture methods to reduce animal use in this area.

journal_name

Trends Biotechnol

journal_title

Trends in biotechnology

authors

Even MS,Sandusky CB,Barnard ND

doi

10.1016/j.tibtech.2006.01.001

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

105-8

issue

3

eissn

0167-7799

issn

1879-3096

pii

S0167-7799(06)00002-3

journal_volume

24

pub_type

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